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Protein

Proline racemase A

Gene

PA45-A

Organism
Trypanosoma cruzi (strain CL Brener)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of L- and D-proline. Secreted isoform 1 contributes to parasite immune evasion by acting as a B-cell mitogen. Probably involved in parasite differentiation and infectivity.3 Publications

Catalytic activityi

L-proline = D-proline.1 Publication

Enzyme regulationi

Inhibited by maleic acid, iodoacetamide, iodoacetate and, most particularly, pyrrole-2-carboxylic acid.1 Publication

Kineticsi

  1. KM=29 mM for L-proline1 Publication
  1. Vmax=0.053 mmol/sec/µg enzyme1 Publication

pH dependencei

Optimum pH is 5.5-7.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei160Proton acceptorCurated1
Binding sitei326Substrate1
Active sitei330Proton donorCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Isomerase

Enzyme and pathway databases

BRENDAi5.1.1.4. 6524.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline racemase A (EC:5.1.1.4)
Alternative name(s):
TcPA45-A
TcPRACA
rTcPA45
Gene namesi
Name:PA45-A
ORF Names:Tc00.1047053506795.80
OrganismiTrypanosoma cruzi (strain CL Brener)
Taxonomic identifieri353153 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum
Proteomesi
  • UP000002296 Componenti: Unassembled WGS sequence

Subcellular locationi

Isoform 1 :
Isoform 2 :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi160C → S: Loss of enzyme activity, without abrogating mitogenic properties. 1 Publication1
Mutagenesisi330C → S: Loss of enzyme activity, without abrogating mitogenic properties. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_500005722532 – 423Proline racemase AAdd BLAST392

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi213N-linked (GlcNAc...)Sequence analysis1
Glycosylationi266N-linked (GlcNAc...)Sequence analysis1
Glycosylationi282N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ4DA80.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

DIPiDIP-61100N.

Structurei

Secondary structure

1423
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi66 – 71Combined sources6
Beta strandi74 – 83Combined sources10
Beta strandi86 – 93Combined sources8
Helixi103 – 113Combined sources11
Helixi115 – 122Combined sources8
Beta strandi132 – 137Combined sources6
Beta strandi145 – 154Combined sources10
Helixi161 – 173Combined sources13
Beta strandi184 – 192Combined sources9
Beta strandi195 – 203Combined sources9
Beta strandi205 – 209Combined sources5
Beta strandi211 – 217Combined sources7
Beta strandi221 – 231Combined sources11
Turni234 – 236Combined sources3
Beta strandi238 – 254Combined sources17
Helixi255 – 258Combined sources4
Beta strandi262 – 264Combined sources3
Helixi267 – 284Combined sources18
Beta strandi297 – 305Combined sources9
Beta strandi313 – 320Combined sources8
Turni321 – 323Combined sources3
Helixi331 – 343Combined sources13
Beta strandi353 – 357Combined sources5
Beta strandi362 – 374Combined sources13
Beta strandi385 – 397Combined sources13
Beta strandi399 – 409Combined sources11
Turni414 – 417Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1W61X-ray2.10A/B32-423[»]
1W62X-ray2.50A/B32-423[»]
ProteinModelPortaliQ4DA80.
SMRiQ4DA80.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ4DA80.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni161 – 162Substrate binding2
Regioni331 – 332Substrate binding2

Sequence similaritiesi

Belongs to the proline racemase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIC6. Eukaryota.
COG3938. LUCA.

Family and domain databases

InterProiIPR008794. Pro_racemase_fam.
[Graphical view]
PfamiPF05544. Pro_racemase. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q4DA80-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRKSVCPKQK FFFSAFPFFF FFCVFPLISR TGQEKLLFDQ KYKIIKGEKK
60 70 80 90 100
EKKKNQRANR REHQQKREIM RFKKSFTCID MHTEGEAARI VTSGLPHIPG
110 120 130 140 150
SNMAEKKAYL QENMDYLRRG IMLEPRGHDD MFGAFLFDPI EEGADLGMVF
160 170 180 190 200
MDTGGYLNMC GHNSIAAVTA AVETGIVSVP AKATNVPVVL DTPAGLVRGT
210 220 230 240 250
AHLQSGTESE VSNASIINVP SFLYQQDVVV VLPKPYGEVR VDIAFGGNFF
260 270 280 290 300
AIVPAEQLGI DISVQNLSRL QEAGELLRTE INRSVKVQHP QLPHINTVDC
310 320 330 340 350
VEIYGPPTNP EANYKNVVIF GNRQADRSPC GTGTSAKMAT LYAKGQLRIG
360 370 380 390 400
ETFVYESILG SLFQGRVLGE ERIPGVKVPV TKDAEEGMLV VTAEITGKAF
410 420
IMGFNTMLFD PTDPFKNGFT LKQ
Length:423
Mass (Da):46,950
Last modified:May 29, 2007 - v2
Checksum:i2BF6DFF8F5AB662E
GO
Isoform 2 (identifier: Q4DA80-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: Missing.

Show »
Length:354
Mass (Da):38,432
Checksum:i88801F775E8B9DAA
GO

Sequence cautioni

The sequence EAN89436 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79I → M in AAF97423 (PubMed:10932226).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0258491 – 69Missing in isoform 2. CuratedAdd BLAST69

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195522 Genomic DNA. Translation: AAF97423.1.
AAHK01000747 Genomic DNA. Translation: EAN89436.1. Sequence problems.
RefSeqiXP_811287.1. XM_806194.1.

Genome annotation databases

EnsemblProtistsiEAN89436; EAN89436; Tc00.1047053506795.80.
GeneIDi3542225.
KEGGitcr:506795.80.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195522 Genomic DNA. Translation: AAF97423.1.
AAHK01000747 Genomic DNA. Translation: EAN89436.1. Sequence problems.
RefSeqiXP_811287.1. XM_806194.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1W61X-ray2.10A/B32-423[»]
1W62X-ray2.50A/B32-423[»]
ProteinModelPortaliQ4DA80.
SMRiQ4DA80.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61100N.

Proteomic databases

PaxDbiQ4DA80.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAN89436; EAN89436; Tc00.1047053506795.80.
GeneIDi3542225.
KEGGitcr:506795.80.

Phylogenomic databases

eggNOGiENOG410IIC6. Eukaryota.
COG3938. LUCA.

Enzyme and pathway databases

BRENDAi5.1.1.4. 6524.

Miscellaneous databases

EvolutionaryTraceiQ4DA80.

Family and domain databases

InterProiIPR008794. Pro_racemase_fam.
[Graphical view]
PfamiPF05544. Pro_racemase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRCMA_TRYCC
AccessioniPrimary (citable) accession number: Q4DA80
Secondary accession number(s): Q9NCP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: November 2, 2016
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.