Q4AE12 (CFI2_FRAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone--flavonone isomerase 2
Short name=Chalcone isomerase 2
EC=5.5.1.6

Gene names

Name:	CHI2
Synonyms:	FrCHI17

Organism

Fragaria ananassa (Strawberry)

Taxonomic identifier

3747 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Rosaceae › Rosoideae › Rosodae › Potentilleae › Fragaria

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin By similarity.
Catalytic activity	A chalcone = a flavanone.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Miscellaneous	Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.
Sequence similarities	Belongs to the chalcone isomerase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	cellular modified amino acid biosynthetic process Inferred from electronic annotation. Source: InterPro flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chalcone isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	Chalcone--flavonone isomerase 2		PRO_0000300837
Sites
	Binding site	48	1	Substrate By similarity
	Binding site	113	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity
	Site	106	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4AE12 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: F1E5838A3F189B34	FASTA	237	25,468
	10 20 30 40 50 60 MAQSVTGIQI GGTSFPPAVK PPGSGNTLFL GGAGVRGMEI QGNFVKFTAI GVYLEDKAVP 70 80 90 100 110 120 ALSVKWKGKT AEELTESVEF FREIVTGPFE KFTQVTMILP LTGQQYSEKV SENCVAIWKK 130 140 150 160 170 180 FGIYTDAEAK AIEKFIEVFK DQTFPPGASI LFTQSPNGSL MIGISKDGSI PEVGNAVIEN 190 200 210 220 230 KLLSESVLES IIGKQGVSPE ARKSVATRLS ELLKESDHCV AGNEKVDECT KEAENKA

References

[1]	"Genes regulating anthocyanin-synthesis pathway in white wild strawberry." Adachi Y., Chiba N., Suzuki G., Kano H., Nakamura S. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Sachinoka. Tissue: Fruit cortical tissue.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB201754 mRNA. Translation: BAE17120.1.
3D structure databases
ProteinModelPortal	Q4AE12.
SMR	Q4AE12. Positions 4-215.
ModBase	Search...
Proteomic databases
PRIDE	Q4AE12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016087. Chalcone_isomerase. IPR016088. Chalcone_isomerase_3-sand. IPR003466. Chalcone_isomerase_subgr. [Graphical view]
Gene3D	G3DSA:3.50.70.10. Chalcone. 1 hit.
Pfam	PF02431. Chalcone. 1 hit. [Graphical view]
SUPFAM	SSF54626. Chalcone. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CFI2_FRAAN

Accession

Primary (citable) accession number: Q4AE12

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 13, 2005
Last modified:	May 31, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program