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Q4ACU6

- SHAN3_MOUSE

UniProt

Q4ACU6 - SHAN3_MOUSE

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Protein

SH3 and multiple ankyrin repeat domains protein 3

Gene

Shank3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.4 Publications

GO - Molecular functioni

  1. GKAP/Homer scaffold activity Source: BHF-UCL
  2. identical protein binding Source: BHF-UCL
  3. ionotropic glutamate receptor binding Source: BHF-UCL
  4. protein C-terminus binding Source: BHF-UCL
  5. scaffold protein binding Source: BHF-UCL
  6. SH3 domain binding Source: BHF-UCL
  7. zinc ion binding Source: BHF-UCL

GO - Biological processi

  1. alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering Source: BHF-UCL
  2. brain morphogenesis Source: BHF-UCL
  3. dendritic spine morphogenesis Source: BHF-UCL
  4. embryonic epithelial tube formation Source: MGI
  5. guanylate kinase-associated protein clustering Source: BHF-UCL
  6. learning Source: BHF-UCL
  7. locomotory exploration behavior Source: BHF-UCL
  8. MAPK cascade Source: MGI
  9. memory Source: BHF-UCL
  10. negative regulation of actin filament bundle assembly Source: MGI
  11. negative regulation of cell volume Source: BHF-UCL
  12. neuromuscular process controlling balance Source: BHF-UCL
  13. N-methyl-D-aspartate receptor clustering Source: BHF-UCL
  14. positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
  15. positive regulation of dendritic spine development Source: BHF-UCL
  16. positive regulation of excitatory postsynaptic membrane potential Source: BHF-UCL
  17. positive regulation of glutamate receptor signaling pathway Source: BHF-UCL
  18. positive regulation of long-term neuronal synaptic plasticity Source: BHF-UCL
  19. positive regulation of synapse structural plasticity Source: BHF-UCL
  20. positive regulation of synaptic transmission, glutamatergic Source: BHF-UCL
  21. postsynaptic density assembly Source: BHF-UCL
  22. regulation of behavioral fear response Source: BHF-UCL
  23. regulation of dendritic spine morphogenesis Source: BHF-UCL
  24. regulation of grooming behavior Source: BHF-UCL
  25. regulation of long term synaptic depression Source: BHF-UCL
  26. regulation of long-term synaptic potentiation Source: BHF-UCL
  27. social behavior Source: BHF-UCL
  28. striatal medium spiny neuron differentiation Source: BHF-UCL
  29. synapse assembly Source: BHF-UCL
  30. vocalization behavior Source: BHF-UCL
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SH3 and multiple ankyrin repeat domains protein 3
Short name:
Shank3
Alternative name(s):
Proline-rich synapse-associated protein 2
Short name:
ProSAP2
SPANK-2
Gene namesi
Name:Shank3
Synonyms:Kiaa1650, Prosap2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1930016. Shank3.

Subcellular locationi

Cytoplasm. Cell junctionsynapse. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density. Cell projectiondendritic spine By similarity
Note: In neuronal cells, extends into the region subjacent to the postsynaptic density (PSD).

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. ciliary membrane Source: BHF-UCL
  3. cytoplasm Source: UniProtKB-KW
  4. excitatory synapse Source: BHF-UCL
  5. neuronal postsynaptic density Source: BHF-UCL
  6. neuron projection Source: BHF-UCL
  7. neuron spine Source: BHF-UCL
  8. plasma membrane Source: BHF-UCL
  9. postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Animals deficient for isoforms 1-7 exhibit self-injourious repetitive grooming and deficits in social interaction. They show defects at striatal synapses and cortico-striatal circuits with an increase in striatal volume, dendritic length, and surface area and a decrease of spine density, length and thickness of PSD. They seem to have an altered molecular composition of postsynaptic machinery in the striatum (PubMed:21423165). In contrast, animals deficient for isoforms 1 and 2 exhibit a normal initiation of social interaction with a perturbed recognition of social novelty (PubMed:21423165). In PubMed:21558424, animals deficient for isoforms 1 and 2 show abnormal social behaviors, communication patterns, repetitive behaviors, learning and memory. In CA1 hippocampus, the synaptic plasticity is impaired with longer dendritic spines, decreased spine density and deficient long-term potentiation. The expression of specific synaptic scaffolding proteins and receptor subunits are altered. Animals deficient for isoforms 1-5 exhibit self-injourious repetitive grooming, brain-region-specific up-regulation of ionotropic glutamate receptors and increased levels of SHANK2 (PubMed:22699619). Animals deficient for predominant isoforms containing exon 21 exhibit motor-coordination deficits, hypersensitivity to heat, novelty avoidance, altered locomotor response to novelty and minimal social abnormalities. They show a decrease in NMDA-AMPA excitatory postsynaptic current ratio in hippocampal CA1, reduced long-term potentiation and deficits in hippocampus-dependent spatial learning and memory (PubMed:24259569).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17301730SH3 and multiple ankyrin repeat domains protein 3PRO_0000291257Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei122 – 1221Phosphotyrosine1 Publication
Modified residuei482 – 4821Phosphoserine1 Publication
Modified residuei555 – 5551Phosphotyrosine1 Publication
Modified residuei1159 – 11591PhosphoserineBy similarity
Modified residuei1163 – 11631PhosphoserineBy similarity
Modified residuei1234 – 12341PhosphothreonineBy similarity
Modified residuei1253 – 12531PhosphoserineBy similarity
Modified residuei1634 – 16341Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4ACU6.
PRIDEiQ4ACU6.

PTM databases

PhosphoSiteiQ4ACU6.

Expressioni

Tissue specificityi

In brain, highly expressed in striatum, thalamus, hippocampus and granule cells of the cerebellum.3 Publications

Developmental stagei

Isoform 3 is weakly expressed at 17 dpc but its expression increases after birth.1 Publication

Gene expression databases

BgeeiQ4ACU6.
CleanExiMM_SHANK3.
ExpressionAtlasiQ4ACU6. baseline and differential.
GenevestigatoriQ4ACU6.

Interactioni

Subunit structurei

May homomultimerize via its SAM domain. Interacts with BAIAP2, DBNL and SLC17A7/VGLUT1. Interacts with DLGAP1/GKAP, GRM1/MGLUR1, GRM5/MGLUR5 and LZTS3 C-termini via its PDZ domain. Interacts with ABI1, HOMER1, HOMER2, HOMER3 and CTTN/cortactin SH3 domain. Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts (via PDZ domain) with the GRIA1 subunit of the AMPA receptor (via PDZ-binding motif). Interacts with WASF1 and CYFIP2; the interactions mediate the association of SHANK3 with the WAVE1 complex. Interacts with ARPC2; the interaction probably mediates the association of SHANK3 with the Arp2/3 complex. Interacts (via ANK repeats) with SHARPIN and SPTAN1. Interacts (via PDZ domain) with ARHGAP44 (probably via PDZ-binding motif); the interaction takes place in dendritic spines and promotes GRIA1 exocytosis.4 Publications

Protein-protein interaction databases

BioGridi208407. 4 interactions.
IntActiQ4ACU6. 5 interactions.

Structurei

Secondary structure

1
1730
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi564 – 57411
Beta strandi582 – 5865
Beta strandi601 – 6033
Beta strandi608 – 6125
Beta strandi614 – 6174
Helixi618 – 6214
Beta strandi628 – 6325
Helixi643 – 6519
Turni652 – 6543
Beta strandi655 – 66511

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3O5NX-ray1.83A/B/C/D/E/F/G/H562-669[»]
ProteinModelPortaliQ4ACU6.
SMRiQ4ACU6. Positions 562-667, 1664-1727.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ4ACU6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati148 – 17831ANK 1Add
BLAST
Repeati182 – 21130ANK 2Add
BLAST
Repeati215 – 24531ANK 3Add
BLAST
Repeati249 – 27830ANK 4Add
BLAST
Repeati282 – 31130ANK 5Add
BLAST
Repeati315 – 34531ANK 6Add
BLAST
Domaini470 – 52960SH3PROSITE-ProRule annotationAdd
BLAST
Domaini570 – 66495PDZPROSITE-ProRule annotationAdd
BLAST
Domaini1667 – 173064SAMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7575Intramolecular interaction with the ANK repeatsBy similarityAdd
BLAST
Regioni677 – 6848Required for interaction with ABI1By similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1494 – 151421Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1410 – 14167SH3-bindingSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi435 – 46228Pro-richAdd
BLAST
Compositional biasi677 – 6804Poly-Pro
Compositional biasi813 – 1349537Pro-richAdd
BLAST

Domaini

In isoform 1, the N-terminal region preceding the ANK repeats interacts with the 6 ANK repeats in an intramolecular manner, thereby restricting access to ligands, such as SHARPIN and SPTAN1.By similarity

Sequence similaritiesi

Belongs to the SHANK family.Curated
Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, SH3 domain

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00510000046474.
HOGENOMiHOG000293276.
HOVERGENiHBG054027.
InParanoidiQ4ACU6.
KOiK15009.
OrthoDBiEOG7GBFW2.
PhylomeDBiQ4ACU6.
TreeFamiTF324593.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.25.40.20. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001478. PDZ.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF00595. PDZ. 1 hit.
PF00536. SAM_1. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 5 hits.
SM00228. PDZ. 1 hit.
SM00454. SAM. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
PS50106. PDZ. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (10)i

Sequence statusi: Complete.

This entry describes 10 isoformsi produced by alternative promoter usage and alternative splicing. Align

Note: Additional isoform seem to exist. These isoforms may be the product of multiple intragenic promoter and/or alternative splicing.2 Publications

Isoform 1 (identifier: Q4ACU6) [UniParc]FASTAAdd to Basket

Also known as: A, Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGPGASAVV VRVGIPDLQQ TKCLRLDPTA PVWAAKQRVL CALNHSLQDA
60 70 80 90 100
LNYGLFQPPS RGRAGKFLDE ERLLQDYPPN LDTPLPYLEF RYKRRVYAQN
110 120 130 140 150
LIDDKQFAKL HTKANLKKFM DYVQLHSTDK VARLLDKGLD PNFHDPDSGE
160 170 180 190 200
CPLSLAAQLD NATDLLKVLR NGGAHLDFRT RDGLTAVHCA TRQRNAGALT
210 220 230 240 250
TLLDLGASPD YKDSRGLTPL YHSALGGGDA LCCELLLHDH AQLGTTDENG
260 270 280 290 300
WQEIHQACRF GHVQHLEHLL FYGANMGAQN ASGNTALHIC ALYNQESCAR
310 320 330 340 350
VLLFRGANKD VRNYNSQTAF QVAIIAGNFE LAEVIKTHKD SDVVPFRETP
360 370 380 390 400
SYAKRRRLAG PSGLASPRPL QRSASDINLK GDQPAASPGP TLRSLPHQLL
410 420 430 440 450
LQRLQEEKDR DRDGELENDI SGPSAGRGGH NKISPSGPGG SGPAPGPGPA
460 470 480 490 500
SPAPPAPPPR GPKRKLYSAV PGRKFIAVKA HSPQGEGEIP LHRGEAVKVL
510 520 530 540 550
SIGEGGFWEG TVKGRTGWFP ADCVEEVQMR QYDTRHETRE DRTKRLFRHY
560 570 580 590 600
TVGSYDSLTS HSDYVIDDKV AILQKRDHEG FGFVLRGAKA ETPIEEFTPT
610 620 630 640 650
PAFPALQYLE SVDVEGVAWR AGLRTGDFLI EVNGVNVVKV GHKQVVGLIR
660 670 680 690 700
QGGNRLVMKV VSVTRKPEED GARRRAPPPP KRAPSTTLTL RSKSMTAELE
710 720 730 740 750
ELASIRRRKG EKLDEILAVA AEPTLRPDIA DADSRAATVK QRPTSRRITP
760 770 780 790 800
AEISSLFERQ GLPGPEKLPG SLRKGIPRTK SVGEDEKLAS LLEGRFPRST
810 820 830 840 850
SMQDTVREGR GIPPPPQTAP PPPPAPYYFD SGPPPTFSPP PPPGRAYDTV
860 870 880 890 900
RSSFKPGLEA RLGAGAAGLY DPSTPLGPLP YPERQKRARS MIILQDSAPE
910 920 930 940 950
VGDVPRPAPA ATPPERPKRR PRPSGPDSPY ANLGAFSASL FAPSKPQRRK
960 970 980 990 1000
SPLVKQLQVE DAQERAALAV GSPGPVGGSF AREPSPTHRG PRPGSLDYSS
1010 1020 1030 1040 1050
GEGLGLTFGG PSPGPVKERR LEERRRSTVF LSVGAIEGSP PSADLPSLQP
1060 1070 1080 1090 1100
SRSIDERLLG TGATTGRDLL LPSPVSALKP LVGGPSLGPS GSTFIHPLTG
1110 1120 1130 1140 1150
KPLDPSSPLA LALAARERAL ASQTPSRSPT PVHSPDADRP GPLFVDVQTR
1160 1170 1180 1190 1200
DSERGPLASP AFSPRSPAWI PVPARREAEK PPREERKSPE DKKSMILSVL
1210 1220 1230 1240 1250
DTSLQRPAGL IVVHATSNGQ EPSRLGAEEE RPGTPELAPA PMQAAAVAEP
1260 1270 1280 1290 1300
MPSPRAQPPG SIPADPGPGQ GSSEEEPELV FAVNLPPAQL SSSDEETREE
1310 1320 1330 1340 1350
LARIGLVPPP EEFANGILLT TPPPGPGPLP TTVPSPASGK PSSELPPAPE
1360 1370 1380 1390 1400
SAADSGVEEA DTRSSSDPHL ETTSTISTVS SMSTLSSESG ELTDTHTSFA
1410 1420 1430 1440 1450
DGHTFLLEKP PVPPKPKLKS PLGKGPVTFR DPLLKQSSDS ELMAQQHHAA
1460 1470 1480 1490 1500
STGLASAAGP ARPRYLFQRR SKLWGDPVES RGLPGPEDDK PTVISELSSR
1510 1520 1530 1540 1550
LQQLNKDTRS LGEEPVGGLG SLLDPAKKSP IAAARLFSSL GELSTISAQR
1560 1570 1580 1590 1600
SPGGPGGGAS YSVRPSGRYP VARRAPSPVK PASLERVEGL GAGVGGAGRP
1610 1620 1630 1640 1650
FGLTPPTILK SSSLSIPHEP KEVRFVVRSV SARSRSPSPS PLPSPSPGSG
1660 1670 1680 1690 1700
PSAGPRRPFQ QKPLQLWSKF DVGDWLESIH LGEHRDRFED HEIEGAHLPA
1710 1720 1730
LTKEDFVELG VTRVGHRMNI ERALRQLDGS
Length:1,730
Mass (Da):185,397
Last modified:February 19, 2014 - v3
Checksum:i1343F857BE3EECD8
GO
Isoform 2 (identifier: Q4ACU6-2) [UniParc]FASTAAdd to Basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-89: MDGPGASAVV...NLDTPLPYLE → MGLCGSLLPTFSLSEQ
     703-710: Missing.
     784-790: EDEKLAS → SSAASVS
     791-1730: Missing.

Note: Produced by alternative promoter usage and alternative splicing.

Show »
Length:709
Mass (Da):77,277
Checksum:i5CF7C46C7DC4B486
GO
Isoform 4 (identifier: Q4ACU6-3) [UniParc]FASTAAdd to Basket

Also known as: C3, Beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-433: MDGPGASAVV...SAGRGGHNKI → MEAPGAGFACPLPPGIASVTYVFVY

Note: Produced by alternative splicing of isoform 3.

Show »
Length:1,322
Mass (Da):140,218
Checksum:i4F90D15CB93B334C
GO
Isoform 5 (identifier: Q4ACU6-6) [UniParc]FASTAAdd to Basket

Also known as: C4

The sequence of this isoform differs from the canonical sequence as follows:
     1-433: MDGPGASAVV...SAGRGGHNKI → MEAPGAGFACPLPPGIASVTYVFVY
     784-790: EDEKLAS → SSAASVS
     791-1730: Missing.

Note: Produced by alternative splicing of isoform 3.

Show »
Length:382
Mass (Da):41,168
Checksum:i27B9B22AB57A32EE
GO
Isoform 6 (identifier: Q4ACU6-7) [UniParc]FASTAAdd to Basket

Also known as: D1

The sequence of this isoform differs from the canonical sequence as follows:
     1-536: MDGPGASAVV...VQMRQYDTRH → MLPA

Note: Produced by alternative promoter usage.

Show »
Length:1,198
Mass (Da):127,378
Checksum:iFBDB744E8EC00FB4
GO
Isoform 7 (identifier: Q4ACU6-8) [UniParc]FASTAAdd to Basket

Also known as: D2

The sequence of this isoform differs from the canonical sequence as follows:
     1-589: MDGPGASAVV...GFGFVLRGAK → MLVNAFYLALPA

Note: Produced by alternative splicing of isoform 6.

Show »
Length:1,153
Mass (Da):122,073
Checksum:iC61D0CF8C4EE18F1
GO
Isoform 8 (identifier: Q4ACU6-9) [UniParc]FASTAAdd to Basket

Also known as: E1

The sequence of this isoform differs from the canonical sequence as follows:
     1-675: MDGPGASAVV...KPEEDGARRR → MKKFASSRSL...RRAKASLFFA

Note: Produced by alternative promoter usage.

Show »
Length:1,112
Mass (Da):118,003
Checksum:i749558749E5CCD1D
GO
Isoform 9 (identifier: Q4ACU6-10) [UniParc]FASTAAdd to Basket

Also known as: E2

The sequence of this isoform differs from the canonical sequence as follows:
     1-675: MDGPGASAVV...KPEEDGARRR → MKKFASSRSL...RRAKASLFFA
     703-710: Missing.

Note: Produced by alternative splicing of isoform 8.

Show »
Length:1,104
Mass (Da):117,078
Checksum:i1133661F14C1619A
GO
Isoform 3 (identifier: Q4ACU6-11) [UniParc]FASTAAdd to Basket

Also known as: C1

The sequence of this isoform differs from the canonical sequence as follows:
     1-528: Missing.

Note: Produced by alternative promoter usage.

Show »
Length:1,202
Mass (Da):128,053
Checksum:iD2247B82002EFA7E
GO
Isoform 10 (identifier: Q4ACU6-12) [UniParc]FASTAAdd to Basket

Also known as: F

The sequence of this isoform differs from the canonical sequence as follows:
     1-890: Missing.

Note: Produced by alternative promoter usage.

Show »
Length:840
Mass (Da):88,196
Checksum:iFD0680BF85041222
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 890890Missing in isoform 10. CuratedVSP_053606Add
BLAST
Alternative sequencei1 – 675675MDGPG…GARRR → MKKFASSRSLNKILAQCDSS SREYEEVQAVERKWHLHLAT PRRLLLDRRAKASLFFA in isoform 8 and isoform 9. CuratedVSP_053607Add
BLAST
Alternative sequencei1 – 589589MDGPG…LRGAK → MLVNAFYLALPA in isoform 7. CuratedVSP_053608Add
BLAST
Alternative sequencei1 – 536536MDGPG…YDTRH → MLPA in isoform 6. CuratedVSP_053609Add
BLAST
Alternative sequencei1 – 528528Missing in isoform 3. 1 PublicationVSP_053610Add
BLAST
Alternative sequencei1 – 433433MDGPG…GHNKI → MEAPGAGFACPLPPGIASVT YVFVY in isoform 4 and isoform 5. 1 PublicationVSP_053611Add
BLAST
Alternative sequencei1 – 8989MDGPG…LPYLE → MGLCGSLLPTFSLSEQ in isoform 2. 1 PublicationVSP_053612Add
BLAST
Alternative sequencei703 – 7108Missing in isoform 2 and isoform 9. 1 PublicationVSP_053613
Alternative sequencei784 – 7907EDEKLAS → SSAASVS in isoform 2 and isoform 5. 1 PublicationVSP_053614
Alternative sequencei791 – 1730940Missing in isoform 2 and isoform 5. 1 PublicationVSP_053615Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ245904 mRNA. Translation: CAB89816.1.
AB841411 mRNA. Translation: BAN67189.1.
AB841412 mRNA. Translation: BAN67190.1.
AC122401 Genomic DNA. No translation available.
AC137513 Genomic DNA. No translation available.
AB231013 mRNA. Translation: BAE16756.1.
AK173228 mRNA. Translation: BAD32506.1.
HQ405757 mRNA. Translation: AEB77764.1.
HQ405758 mRNA. Translation: AEB77765.1.
HQ405759 mRNA. Translation: AEB77766.1.
HQ405760 mRNA. Translation: AEB77767.1.
HQ405761 mRNA. Translation: AEB77768.1.
HQ405762 mRNA. Translation: AEB77769.1.
HQ405763 mRNA. Translation: AEB77770.1.
CCDSiCCDS27754.1. [Q4ACU6-1]
RefSeqiNP_067398.2. NM_021423.3. [Q4ACU6-1]
UniGeneiMm.146855.

Genome annotation databases

EnsembliENSMUST00000039074; ENSMUSP00000048062; ENSMUSG00000022623. [Q4ACU6-1]
ENSMUST00000066545; ENSMUSP00000064477; ENSMUSG00000022623. [Q4ACU6-2]
GeneIDi58234.
KEGGimmu:58234.
UCSCiuc007xha.2. mouse. [Q4ACU6-1]
uc007xhb.2. mouse.

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ245904 mRNA. Translation: CAB89816.1 .
AB841411 mRNA. Translation: BAN67189.1 .
AB841412 mRNA. Translation: BAN67190.1 .
AC122401 Genomic DNA. No translation available.
AC137513 Genomic DNA. No translation available.
AB231013 mRNA. Translation: BAE16756.1 .
AK173228 mRNA. Translation: BAD32506.1 .
HQ405757 mRNA. Translation: AEB77764.1 .
HQ405758 mRNA. Translation: AEB77765.1 .
HQ405759 mRNA. Translation: AEB77766.1 .
HQ405760 mRNA. Translation: AEB77767.1 .
HQ405761 mRNA. Translation: AEB77768.1 .
HQ405762 mRNA. Translation: AEB77769.1 .
HQ405763 mRNA. Translation: AEB77770.1 .
CCDSi CCDS27754.1. [Q4ACU6-1 ]
RefSeqi NP_067398.2. NM_021423.3. [Q4ACU6-1 ]
UniGenei Mm.146855.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
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Entry Method Resolution (Å) Chain Positions PDBsum
3O5N X-ray 1.83 A/B/C/D/E/F/G/H 562-669 [» ]
ProteinModelPortali Q4ACU6.
SMRi Q4ACU6. Positions 562-667, 1664-1727.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 208407. 4 interactions.
IntActi Q4ACU6. 5 interactions.

PTM databases

PhosphoSitei Q4ACU6.

Proteomic databases

PaxDbi Q4ACU6.
PRIDEi Q4ACU6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000039074 ; ENSMUSP00000048062 ; ENSMUSG00000022623 . [Q4ACU6-1 ]
ENSMUST00000066545 ; ENSMUSP00000064477 ; ENSMUSG00000022623 . [Q4ACU6-2 ]
GeneIDi 58234.
KEGGi mmu:58234.
UCSCi uc007xha.2. mouse. [Q4ACU6-1 ]
uc007xhb.2. mouse.

Organism-specific databases

CTDi 85358.
MGIi MGI:1930016. Shank3.
Rougei Search...

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00510000046474.
HOGENOMi HOG000293276.
HOVERGENi HBG054027.
InParanoidi Q4ACU6.
KOi K15009.
OrthoDBi EOG7GBFW2.
PhylomeDBi Q4ACU6.
TreeFami TF324593.

Miscellaneous databases

ChiTaRSi SHANK3. mouse.
EvolutionaryTracei Q4ACU6.
NextBioi 314265.
PROi Q4ACU6.
SOURCEi Search...

Gene expression databases

Bgeei Q4ACU6.
CleanExi MM_SHANK3.
ExpressionAtlasi Q4ACU6. baseline and differential.
Genevestigatori Q4ACU6.

Family and domain databases

Gene3Di 1.10.150.50. 1 hit.
1.25.40.20. 1 hit.
2.30.42.10. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001478. PDZ.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF12796. Ank_2. 2 hits.
PF00595. PDZ. 1 hit.
PF00536. SAM_1. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view ]
SMARTi SM00248. ANK. 5 hits.
SM00228. PDZ. 1 hit.
SM00454. SAM. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
PS50106. PDZ. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Interaction of the Na+/Phosphate cotransporter type II with rSHANK."
    Dehmelt L., Nalbant P., Werner A.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: C57B16/J.
    Tissue: Kidney.
  2. "Direct interaction of post-synaptic density-95/Dlg/ZO-1 domain-containing synaptic molecule Shank3 with GluR1 alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor."
    Uchino S., Wada H., Honda S., Nakamura Y., Ondo Y., Uchiyama T., Tsutsumi M., Suzuki E., Hirasawa T., Kohsaka S.
    J. Neurochem. 97:1203-1214(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH GRIA1, SUBCELLULAR LOCATION.
  3. "Identification of two novel Shank3 transcripts in the developing mouse neocortex."
    Waga C., Asano H., Sanagi T., Suzuki E., Nakamura Y., Tsuchiya A., Itoh M., Goto Y.I., Kohsaka S., Uchino S.
    J. Neurochem. 128:280-293(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 875-1730.
    Tissue: Brain.
  6. Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3; 6; 7; 8 AND 9), ALTERNATIVE SPLICING, FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INTERACTION WITH HOMER1.
  7. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-482, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1634, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-122 AND TYR-555, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  10. Cited for: ALTERNATIVE PROMOTER USAGE.
  11. "Shank3 mutant mice display autistic-like behaviours and striatal dysfunction."
    Peca J., Feliciano C., Ting J.T., Wang W., Wells M.F., Venkatraman T.N., Lascola C.D., Fu Z., Feng G.
    Nature 472:437-442(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  12. Cited for: DISRUPTION PHENOTYPE.
  13. "Shank3-Rich2 interaction regulates AMPA receptor recycling and synaptic long-term potentiation."
    Raynaud F., Janossy A., Dahl J., Bertaso F., Perroy J., Varrault A., Vidal M., Worley P.F., Boeckers T.M., Bockaert J., Marin P., Fagni L., Homburger V.
    J. Neurosci. 33:9699-9715(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN AMPA RECEPTOR SIGNALING, INTERACTION WITH ARHGAP44.
  14. "Loss of predominant shank3 isoforms results in hippocampus-dependent impairments in behavior and synaptic transmission."
    Kouser M., Speed H.E., Dewey C.M., Reimers J.M., Widman A.J., Gupta N., Liu S., Jaramillo T.C., Bangash M., Xiao B., Worley P.F., Powell C.M.
    J. Neurosci. 33:18448-18468(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  15. "SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties."
    Han K., Holder J.L. Jr., Schaaf C.P., Lu H., Chen H., Kang H., Tang J., Wu Z., Hao S., Cheung S.W., Yu P., Sun H., Breman A.M., Patel A., Lu H.C., Zoghbi H.Y.
    Nature 503:72-77(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ACTIN-BINDING, INTERACTION WITH ARPC2; CYFIP2; DLG4; GKAP1; HOMER1 AND SLC17A7.

Entry informationi

Entry nameiSHAN3_MOUSE
AccessioniPrimary (citable) accession number: Q4ACU6
Secondary accession number(s): F8S0X0
, F8S0X2, F8S0X3, F8S0X5, F8S0X6, Q69ZD8, Q9JJZ3, S6BMD3, S6CCV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: February 19, 2014
Last modified: October 29, 2014
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3