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Q4ACU6

- SHAN3_MOUSE

UniProt

Q4ACU6 - SHAN3_MOUSE

Protein

SH3 and multiple ankyrin repeat domains protein 3

Gene

Shank3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 3 (19 Feb 2014)
      Previous versions | rss
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    Functioni

    Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.4 Publications

    GO - Molecular functioni

    1. GKAP/Homer scaffold activity Source: BHF-UCL
    2. identical protein binding Source: BHF-UCL
    3. ionotropic glutamate receptor binding Source: BHF-UCL
    4. protein binding Source: MGI
    5. protein C-terminus binding Source: BHF-UCL
    6. scaffold protein binding Source: BHF-UCL
    7. SH3 domain binding Source: BHF-UCL
    8. zinc ion binding Source: BHF-UCL

    GO - Biological processi

    1. alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering Source: BHF-UCL
    2. brain morphogenesis Source: BHF-UCL
    3. dendritic spine morphogenesis Source: BHF-UCL
    4. embryonic epithelial tube formation Source: MGI
    5. guanylate kinase-associated protein clustering Source: BHF-UCL
    6. learning Source: BHF-UCL
    7. locomotory exploration behavior Source: BHF-UCL
    8. MAPK cascade Source: MGI
    9. memory Source: BHF-UCL
    10. negative regulation of actin filament bundle assembly Source: MGI
    11. negative regulation of cell volume Source: BHF-UCL
    12. neuromuscular process controlling balance Source: BHF-UCL
    13. N-methyl-D-aspartate receptor clustering Source: BHF-UCL
    14. positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
    15. positive regulation of dendritic spine development Source: BHF-UCL
    16. positive regulation of excitatory postsynaptic membrane potential Source: BHF-UCL
    17. positive regulation of glutamate receptor signaling pathway Source: BHF-UCL
    18. positive regulation of long-term neuronal synaptic plasticity Source: BHF-UCL
    19. positive regulation of synapse structural plasticity Source: BHF-UCL
    20. positive regulation of synaptic transmission, glutamatergic Source: BHF-UCL
    21. postsynaptic density assembly Source: BHF-UCL
    22. protein oligomerization Source: Ensembl
    23. regulation of behavioral fear response Source: BHF-UCL
    24. regulation of dendritic spine morphogenesis Source: BHF-UCL
    25. regulation of grooming behavior Source: BHF-UCL
    26. regulation of long term synaptic depression Source: BHF-UCL
    27. regulation of long-term synaptic potentiation Source: BHF-UCL
    28. social behavior Source: BHF-UCL
    29. striatal medium spiny neuron differentiation Source: BHF-UCL
    30. synapse assembly Source: BHF-UCL
    31. vocalization behavior Source: BHF-UCL

    Keywords - Ligandi

    Actin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SH3 and multiple ankyrin repeat domains protein 3
    Short name:
    Shank3
    Alternative name(s):
    Proline-rich synapse-associated protein 2
    Short name:
    ProSAP2
    SPANK-2
    Gene namesi
    Name:Shank3
    Synonyms:Kiaa1650, Prosap2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1930016. Shank3.

    Subcellular locationi

    Cytoplasm. Cell junctionsynapse. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density. Cell projectiondendritic spine By similarity
    Note: In neuronal cells, extends into the region subjacent to the postsynaptic density (PSD).

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. ciliary membrane Source: BHF-UCL
    3. cytoplasm Source: UniProtKB-SubCell
    4. dendritic spine Source: UniProtKB-SubCell
    5. excitatory synapse Source: BHF-UCL
    6. neuronal postsynaptic density Source: BHF-UCL
    7. neuron projection Source: BHF-UCL
    8. neuron spine Source: BHF-UCL
    9. plasma membrane Source: BHF-UCL
    10. postsynaptic membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Disruption phenotypei

    Animals deficient for isoforms 1-7 exhibit self-injourious repetitive grooming and deficits in social interaction. They show defects at striatal synapses and cortico-striatal circuits with an increase in striatal volume, dendritic length, and surface area and a decrease of spine density, length and thickness of PSD. They seem to have an altered molecular composition of postsynaptic machinery in the striatum (PubMed:21423165). In contrast, animals deficient for isoforms 1 and 2 exhibit a normal initiation of social interaction with a perturbed recognition of social novelty (PubMed:21423165). In PubMed:21558424, animals deficient for isoforms 1 and 2 show abnormal social behaviors, communication patterns, repetitive behaviors, learning and memory. In CA1 hippocampus, the synaptic plasticity is impaired with longer dendritic spines, decreased spine density and deficient long-term potentiation. The expression of specific synaptic scaffolding proteins and receptor subunits are altered. Animals deficient for isoforms 1-5 exhibit self-injourious repetitive grooming, brain-region-specific up-regulation of ionotropic glutamate receptors and increased levels of SHANK2 (PubMed:22699619). Animals deficient for predominant isoforms containing exon 21 exhibit motor-coordination deficits, hypersensitivity to heat, novelty avoidance, altered locomotor response to novelty and minimal social abnormalities. They show a decrease in NMDA-AMPA excitatory postsynaptic current ratio in hippocampal CA1, reduced long-term potentiation and deficits in hippocampus-dependent spatial learning and memory (PubMed:24259569).4 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 17301730SH3 and multiple ankyrin repeat domains protein 3PRO_0000291257Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei122 – 1221Phosphotyrosine1 Publication
    Modified residuei482 – 4821Phosphoserine1 Publication
    Modified residuei555 – 5551Phosphotyrosine1 Publication
    Modified residuei1159 – 11591PhosphoserineBy similarity
    Modified residuei1163 – 11631PhosphoserineBy similarity
    Modified residuei1234 – 12341PhosphothreonineBy similarity
    Modified residuei1253 – 12531PhosphoserineBy similarity
    Modified residuei1634 – 16341Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ4ACU6.
    PRIDEiQ4ACU6.

    PTM databases

    PhosphoSiteiQ4ACU6.

    Expressioni

    Tissue specificityi

    In brain, highly expressed in striatum, thalamus, hippocampus and granule cells of the cerebellum.3 Publications

    Developmental stagei

    Isoform 3 is weakly expressed at 17 dpc but its expression increases after birth.1 Publication

    Gene expression databases

    ArrayExpressiQ4ACU6.
    BgeeiQ4ACU6.
    CleanExiMM_SHANK3.
    GenevestigatoriQ4ACU6.

    Interactioni

    Subunit structurei

    May homomultimerize via its SAM domain. Interacts with BAIAP2, DBNL and SLC17A7/VGLUT1. Interacts with DLGAP1/GKAP, GRM1/MGLUR1, GRM5/MGLUR5 and LZTS3 C-termini via its PDZ domain. Interacts with ABI1, HOMER1, HOMER2, HOMER3 and CTTN/cortactin SH3 domain. Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts (via PDZ domain) with the GRIA1 subunit of the AMPA receptor (via PDZ-binding motif). Interacts with WASF1 and CYFIP2; the interactions mediate the association of SHANK3 with the WAVE1 complex. Interacts with ARPC2; the interaction probably mediates the association of SHANK3 with the Arp2/3 complex. Interacts (via ANK repeats) with SHARPIN and SPTAN1. Interacts (via PDZ domain) with ARHGAP44 (probably via PDZ-binding motif); the interaction takes place in dendritic spines and promotes GRIA1 exocytosis.4 Publications

    Protein-protein interaction databases

    BioGridi208407. 4 interactions.
    IntActiQ4ACU6. 5 interactions.

    Structurei

    Secondary structure

    1
    1730
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi564 – 57411
    Beta strandi582 – 5865
    Beta strandi601 – 6033
    Beta strandi608 – 6125
    Beta strandi614 – 6174
    Helixi618 – 6214
    Beta strandi628 – 6325
    Helixi643 – 6519
    Turni652 – 6543
    Beta strandi655 – 66511

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3O5NX-ray1.83A/B/C/D/E/F/G/H562-669[»]
    ProteinModelPortaliQ4ACU6.
    SMRiQ4ACU6. Positions 562-667, 1664-1727.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ4ACU6.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati148 – 17831ANK 1Add
    BLAST
    Repeati182 – 21130ANK 2Add
    BLAST
    Repeati215 – 24531ANK 3Add
    BLAST
    Repeati249 – 27830ANK 4Add
    BLAST
    Repeati282 – 31130ANK 5Add
    BLAST
    Repeati315 – 34531ANK 6Add
    BLAST
    Domaini470 – 52960SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini570 – 66495PDZPROSITE-ProRule annotationAdd
    BLAST
    Domaini1667 – 173064SAMPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 7575Intramolecular interaction with the ANK repeatsBy similarityAdd
    BLAST
    Regioni677 – 6848Required for interaction with ABI1By similarity

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1494 – 151421Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1410 – 14167SH3-bindingSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi435 – 46228Pro-richAdd
    BLAST
    Compositional biasi677 – 6804Poly-Pro
    Compositional biasi813 – 1349537Pro-richAdd
    BLAST

    Domaini

    In isoform 1, the N-terminal region preceding the ANK repeats interacts with the 6 ANK repeats in an intramolecular manner, thereby restricting access to ligands, such as SHARPIN and SPTAN1.By similarity

    Sequence similaritiesi

    Belongs to the SHANK family.Curated
    Contains 6 ANK repeats.PROSITE-ProRule annotation
    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
    Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Coiled coil, Repeat, SH3 domain

    Phylogenomic databases

    eggNOGiCOG0666.
    GeneTreeiENSGT00510000046474.
    HOGENOMiHOG000293276.
    HOVERGENiHBG054027.
    InParanoidiQ4ACU6.
    KOiK15009.
    OrthoDBiEOG7GBFW2.
    PhylomeDBiQ4ACU6.
    TreeFamiTF324593.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    1.25.40.20. 1 hit.
    2.30.42.10. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR001478. PDZ.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR021129. SAM_type1.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF12796. Ank_2. 2 hits.
    PF00595. PDZ. 1 hit.
    PF00536. SAM_1. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 5 hits.
    SM00228. PDZ. 1 hit.
    SM00454. SAM. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    SSF48403. SSF48403. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF50156. SSF50156. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 4 hits.
    PS50106. PDZ. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequences (10)i

    Sequence statusi: Complete.

    This entry describes 10 isoformsi produced by alternative promoter usage and alternative splicing. Align

    Note: Additional isoform seem to exist. These isoforms may be the product of multiple intragenic promoter and/or alternative splicing.2 Publications

    Isoform 1 (identifier: Q4ACU6-1) [UniParc]FASTAAdd to Basket

    Also known as: A, Alpha

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDGPGASAVV VRVGIPDLQQ TKCLRLDPTA PVWAAKQRVL CALNHSLQDA     50
    LNYGLFQPPS RGRAGKFLDE ERLLQDYPPN LDTPLPYLEF RYKRRVYAQN 100
    LIDDKQFAKL HTKANLKKFM DYVQLHSTDK VARLLDKGLD PNFHDPDSGE 150
    CPLSLAAQLD NATDLLKVLR NGGAHLDFRT RDGLTAVHCA TRQRNAGALT 200
    TLLDLGASPD YKDSRGLTPL YHSALGGGDA LCCELLLHDH AQLGTTDENG 250
    WQEIHQACRF GHVQHLEHLL FYGANMGAQN ASGNTALHIC ALYNQESCAR 300
    VLLFRGANKD VRNYNSQTAF QVAIIAGNFE LAEVIKTHKD SDVVPFRETP 350
    SYAKRRRLAG PSGLASPRPL QRSASDINLK GDQPAASPGP TLRSLPHQLL 400
    LQRLQEEKDR DRDGELENDI SGPSAGRGGH NKISPSGPGG SGPAPGPGPA 450
    SPAPPAPPPR GPKRKLYSAV PGRKFIAVKA HSPQGEGEIP LHRGEAVKVL 500
    SIGEGGFWEG TVKGRTGWFP ADCVEEVQMR QYDTRHETRE DRTKRLFRHY 550
    TVGSYDSLTS HSDYVIDDKV AILQKRDHEG FGFVLRGAKA ETPIEEFTPT 600
    PAFPALQYLE SVDVEGVAWR AGLRTGDFLI EVNGVNVVKV GHKQVVGLIR 650
    QGGNRLVMKV VSVTRKPEED GARRRAPPPP KRAPSTTLTL RSKSMTAELE 700
    ELASIRRRKG EKLDEILAVA AEPTLRPDIA DADSRAATVK QRPTSRRITP 750
    AEISSLFERQ GLPGPEKLPG SLRKGIPRTK SVGEDEKLAS LLEGRFPRST 800
    SMQDTVREGR GIPPPPQTAP PPPPAPYYFD SGPPPTFSPP PPPGRAYDTV 850
    RSSFKPGLEA RLGAGAAGLY DPSTPLGPLP YPERQKRARS MIILQDSAPE 900
    VGDVPRPAPA ATPPERPKRR PRPSGPDSPY ANLGAFSASL FAPSKPQRRK 950
    SPLVKQLQVE DAQERAALAV GSPGPVGGSF AREPSPTHRG PRPGSLDYSS 1000
    GEGLGLTFGG PSPGPVKERR LEERRRSTVF LSVGAIEGSP PSADLPSLQP 1050
    SRSIDERLLG TGATTGRDLL LPSPVSALKP LVGGPSLGPS GSTFIHPLTG 1100
    KPLDPSSPLA LALAARERAL ASQTPSRSPT PVHSPDADRP GPLFVDVQTR 1150
    DSERGPLASP AFSPRSPAWI PVPARREAEK PPREERKSPE DKKSMILSVL 1200
    DTSLQRPAGL IVVHATSNGQ EPSRLGAEEE RPGTPELAPA PMQAAAVAEP 1250
    MPSPRAQPPG SIPADPGPGQ GSSEEEPELV FAVNLPPAQL SSSDEETREE 1300
    LARIGLVPPP EEFANGILLT TPPPGPGPLP TTVPSPASGK PSSELPPAPE 1350
    SAADSGVEEA DTRSSSDPHL ETTSTISTVS SMSTLSSESG ELTDTHTSFA 1400
    DGHTFLLEKP PVPPKPKLKS PLGKGPVTFR DPLLKQSSDS ELMAQQHHAA 1450
    STGLASAAGP ARPRYLFQRR SKLWGDPVES RGLPGPEDDK PTVISELSSR 1500
    LQQLNKDTRS LGEEPVGGLG SLLDPAKKSP IAAARLFSSL GELSTISAQR 1550
    SPGGPGGGAS YSVRPSGRYP VARRAPSPVK PASLERVEGL GAGVGGAGRP 1600
    FGLTPPTILK SSSLSIPHEP KEVRFVVRSV SARSRSPSPS PLPSPSPGSG 1650
    PSAGPRRPFQ QKPLQLWSKF DVGDWLESIH LGEHRDRFED HEIEGAHLPA 1700
    LTKEDFVELG VTRVGHRMNI ERALRQLDGS 1730
    Length:1,730
    Mass (Da):185,397
    Last modified:February 19, 2014 - v3
    Checksum:i1343F857BE3EECD8
    GO
    Isoform 2 (identifier: Q4ACU6-2) [UniParc]FASTAAdd to Basket

    Also known as: B

    The sequence of this isoform differs from the canonical sequence as follows:
         1-89: MDGPGASAVV...NLDTPLPYLE → MGLCGSLLPTFSLSEQ
         703-710: Missing.
         784-790: EDEKLAS → SSAASVS
         791-1730: Missing.

    Note: Produced by alternative promoter usage and alternative splicing.

    Show »
    Length:709
    Mass (Da):77,277
    Checksum:i5CF7C46C7DC4B486
    GO
    Isoform 4 (identifier: Q4ACU6-3) [UniParc]FASTAAdd to Basket

    Also known as: C3, Beta

    The sequence of this isoform differs from the canonical sequence as follows:
         1-433: MDGPGASAVV...SAGRGGHNKI → MEAPGAGFACPLPPGIASVTYVFVY

    Note: Produced by alternative splicing of isoform 3.

    Show »
    Length:1,322
    Mass (Da):140,218
    Checksum:i4F90D15CB93B334C
    GO
    Isoform 5 (identifier: Q4ACU6-6) [UniParc]FASTAAdd to Basket

    Also known as: C4

    The sequence of this isoform differs from the canonical sequence as follows:
         1-433: MDGPGASAVV...SAGRGGHNKI → MEAPGAGFACPLPPGIASVTYVFVY
         784-790: EDEKLAS → SSAASVS
         791-1730: Missing.

    Note: Produced by alternative splicing of isoform 3.

    Show »
    Length:382
    Mass (Da):41,168
    Checksum:i27B9B22AB57A32EE
    GO
    Isoform 6 (identifier: Q4ACU6-7) [UniParc]FASTAAdd to Basket

    Also known as: D1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-536: MDGPGASAVV...VQMRQYDTRH → MLPA

    Note: Produced by alternative promoter usage.

    Show »
    Length:1,198
    Mass (Da):127,378
    Checksum:iFBDB744E8EC00FB4
    GO
    Isoform 7 (identifier: Q4ACU6-8) [UniParc]FASTAAdd to Basket

    Also known as: D2

    The sequence of this isoform differs from the canonical sequence as follows:
         1-589: MDGPGASAVV...GFGFVLRGAK → MLVNAFYLALPA

    Note: Produced by alternative splicing of isoform 6.

    Show »
    Length:1,153
    Mass (Da):122,073
    Checksum:iC61D0CF8C4EE18F1
    GO
    Isoform 8 (identifier: Q4ACU6-9) [UniParc]FASTAAdd to Basket

    Also known as: E1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-675: MDGPGASAVV...KPEEDGARRR → MKKFASSRSL...RRAKASLFFA

    Note: Produced by alternative promoter usage.

    Show »
    Length:1,112
    Mass (Da):118,003
    Checksum:i749558749E5CCD1D
    GO
    Isoform 9 (identifier: Q4ACU6-10) [UniParc]FASTAAdd to Basket

    Also known as: E2

    The sequence of this isoform differs from the canonical sequence as follows:
         1-675: MDGPGASAVV...KPEEDGARRR → MKKFASSRSL...RRAKASLFFA
         703-710: Missing.

    Note: Produced by alternative splicing of isoform 8.

    Show »
    Length:1,104
    Mass (Da):117,078
    Checksum:i1133661F14C1619A
    GO
    Isoform 3 (identifier: Q4ACU6-11) [UniParc]FASTAAdd to Basket

    Also known as: C1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-528: Missing.

    Note: Produced by alternative promoter usage.

    Show »
    Length:1,202
    Mass (Da):128,053
    Checksum:iD2247B82002EFA7E
    GO
    Isoform 10 (identifier: Q4ACU6-12) [UniParc]FASTAAdd to Basket

    Also known as: F

    The sequence of this isoform differs from the canonical sequence as follows:
         1-890: Missing.

    Note: Produced by alternative promoter usage.

    Show »
    Length:840
    Mass (Da):88,196
    Checksum:iFD0680BF85041222
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 890890Missing in isoform 10. CuratedVSP_053606Add
    BLAST
    Alternative sequencei1 – 675675MDGPG…GARRR → MKKFASSRSLNKILAQCDSS SREYEEVQAVERKWHLHLAT PRRLLLDRRAKASLFFA in isoform 8 and isoform 9. CuratedVSP_053607Add
    BLAST
    Alternative sequencei1 – 589589MDGPG…LRGAK → MLVNAFYLALPA in isoform 7. CuratedVSP_053608Add
    BLAST
    Alternative sequencei1 – 536536MDGPG…YDTRH → MLPA in isoform 6. CuratedVSP_053609Add
    BLAST
    Alternative sequencei1 – 528528Missing in isoform 3. 1 PublicationVSP_053610Add
    BLAST
    Alternative sequencei1 – 433433MDGPG…GHNKI → MEAPGAGFACPLPPGIASVT YVFVY in isoform 4 and isoform 5. 1 PublicationVSP_053611Add
    BLAST
    Alternative sequencei1 – 8989MDGPG…LPYLE → MGLCGSLLPTFSLSEQ in isoform 2. 1 PublicationVSP_053612Add
    BLAST
    Alternative sequencei703 – 7108Missing in isoform 2 and isoform 9. 1 PublicationVSP_053613
    Alternative sequencei784 – 7907EDEKLAS → SSAASVS in isoform 2 and isoform 5. 1 PublicationVSP_053614
    Alternative sequencei791 – 1730940Missing in isoform 2 and isoform 5. 1 PublicationVSP_053615Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ245904 mRNA. Translation: CAB89816.1.
    AB841411 mRNA. Translation: BAN67189.1.
    AB841412 mRNA. Translation: BAN67190.1.
    AC122401 Genomic DNA. No translation available.
    AC137513 Genomic DNA. No translation available.
    AB231013 mRNA. Translation: BAE16756.1.
    AK173228 mRNA. Translation: BAD32506.1.
    HQ405757 mRNA. Translation: AEB77764.1.
    HQ405758 mRNA. Translation: AEB77765.1.
    HQ405759 mRNA. Translation: AEB77766.1.
    HQ405760 mRNA. Translation: AEB77767.1.
    HQ405761 mRNA. Translation: AEB77768.1.
    HQ405762 mRNA. Translation: AEB77769.1.
    HQ405763 mRNA. Translation: AEB77770.1.
    CCDSiCCDS27754.1. [Q4ACU6-1]
    RefSeqiNP_067398.2. NM_021423.3. [Q4ACU6-1]
    UniGeneiMm.146855.

    Genome annotation databases

    EnsembliENSMUST00000039074; ENSMUSP00000048062; ENSMUSG00000022623. [Q4ACU6-1]
    ENSMUST00000066545; ENSMUSP00000064477; ENSMUSG00000022623. [Q4ACU6-2]
    GeneIDi58234.
    KEGGimmu:58234.
    UCSCiuc007xha.2. mouse. [Q4ACU6-1]
    uc007xhb.2. mouse.

    Keywords - Coding sequence diversityi

    Alternative promoter usage, Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ245904 mRNA. Translation: CAB89816.1 .
    AB841411 mRNA. Translation: BAN67189.1 .
    AB841412 mRNA. Translation: BAN67190.1 .
    AC122401 Genomic DNA. No translation available.
    AC137513 Genomic DNA. No translation available.
    AB231013 mRNA. Translation: BAE16756.1 .
    AK173228 mRNA. Translation: BAD32506.1 .
    HQ405757 mRNA. Translation: AEB77764.1 .
    HQ405758 mRNA. Translation: AEB77765.1 .
    HQ405759 mRNA. Translation: AEB77766.1 .
    HQ405760 mRNA. Translation: AEB77767.1 .
    HQ405761 mRNA. Translation: AEB77768.1 .
    HQ405762 mRNA. Translation: AEB77769.1 .
    HQ405763 mRNA. Translation: AEB77770.1 .
    CCDSi CCDS27754.1. [Q4ACU6-1 ]
    RefSeqi NP_067398.2. NM_021423.3. [Q4ACU6-1 ]
    UniGenei Mm.146855.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3O5N X-ray 1.83 A/B/C/D/E/F/G/H 562-669 [» ]
    ProteinModelPortali Q4ACU6.
    SMRi Q4ACU6. Positions 562-667, 1664-1727.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208407. 4 interactions.
    IntActi Q4ACU6. 5 interactions.

    PTM databases

    PhosphoSitei Q4ACU6.

    Proteomic databases

    PaxDbi Q4ACU6.
    PRIDEi Q4ACU6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000039074 ; ENSMUSP00000048062 ; ENSMUSG00000022623 . [Q4ACU6-1 ]
    ENSMUST00000066545 ; ENSMUSP00000064477 ; ENSMUSG00000022623 . [Q4ACU6-2 ]
    GeneIDi 58234.
    KEGGi mmu:58234.
    UCSCi uc007xha.2. mouse. [Q4ACU6-1 ]
    uc007xhb.2. mouse.

    Organism-specific databases

    CTDi 85358.
    MGIi MGI:1930016. Shank3.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG0666.
    GeneTreei ENSGT00510000046474.
    HOGENOMi HOG000293276.
    HOVERGENi HBG054027.
    InParanoidi Q4ACU6.
    KOi K15009.
    OrthoDBi EOG7GBFW2.
    PhylomeDBi Q4ACU6.
    TreeFami TF324593.

    Miscellaneous databases

    ChiTaRSi SHANK3. mouse.
    EvolutionaryTracei Q4ACU6.
    NextBioi 314265.
    PROi Q4ACU6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q4ACU6.
    Bgeei Q4ACU6.
    CleanExi MM_SHANK3.
    Genevestigatori Q4ACU6.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    1.25.40.20. 1 hit.
    2.30.42.10. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR001478. PDZ.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR021129. SAM_type1.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF12796. Ank_2. 2 hits.
    PF00595. PDZ. 1 hit.
    PF00536. SAM_1. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view ]
    SMARTi SM00248. ANK. 5 hits.
    SM00228. PDZ. 1 hit.
    SM00454. SAM. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    SSF48403. SSF48403. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF50156. SSF50156. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 4 hits.
    PS50106. PDZ. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Interaction of the Na+/Phosphate cotransporter type II with rSHANK."
      Dehmelt L., Nalbant P., Werner A.
      Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: C57B16/J.
      Tissue: Kidney.
    2. "Direct interaction of post-synaptic density-95/Dlg/ZO-1 domain-containing synaptic molecule Shank3 with GluR1 alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor."
      Uchino S., Wada H., Honda S., Nakamura Y., Ondo Y., Uchiyama T., Tsutsumi M., Suzuki E., Hirasawa T., Kohsaka S.
      J. Neurochem. 97:1203-1214(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH GRIA1, SUBCELLULAR LOCATION.
    3. "Identification of two novel Shank3 transcripts in the developing mouse neocortex."
      Waga C., Asano H., Sanagi T., Suzuki E., Nakamura Y., Tsuchiya A., Itoh M., Goto Y.I., Kohsaka S., Uchino S.
      J. Neurochem. 128:280-293(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
      DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 875-1730.
      Tissue: Brain.
    6. Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3; 6; 7; 8 AND 9), ALTERNATIVE SPLICING, FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INTERACTION WITH HOMER1.
    7. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-482, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1634, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    9. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-122 AND TYR-555, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    10. Cited for: ALTERNATIVE PROMOTER USAGE.
    11. "Shank3 mutant mice display autistic-like behaviours and striatal dysfunction."
      Peca J., Feliciano C., Ting J.T., Wang W., Wells M.F., Venkatraman T.N., Lascola C.D., Fu Z., Feng G.
      Nature 472:437-442(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    12. Cited for: DISRUPTION PHENOTYPE.
    13. "Shank3-Rich2 interaction regulates AMPA receptor recycling and synaptic long-term potentiation."
      Raynaud F., Janossy A., Dahl J., Bertaso F., Perroy J., Varrault A., Vidal M., Worley P.F., Boeckers T.M., Bockaert J., Marin P., Fagni L., Homburger V.
      J. Neurosci. 33:9699-9715(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN AMPA RECEPTOR SIGNALING, INTERACTION WITH ARHGAP44.
    14. "Loss of predominant shank3 isoforms results in hippocampus-dependent impairments in behavior and synaptic transmission."
      Kouser M., Speed H.E., Dewey C.M., Reimers J.M., Widman A.J., Gupta N., Liu S., Jaramillo T.C., Bangash M., Xiao B., Worley P.F., Powell C.M.
      J. Neurosci. 33:18448-18468(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    15. "SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties."
      Han K., Holder J.L. Jr., Schaaf C.P., Lu H., Chen H., Kang H., Tang J., Wu Z., Hao S., Cheung S.W., Yu P., Sun H., Breman A.M., Patel A., Lu H.C., Zoghbi H.Y.
      Nature 503:72-77(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ACTIN-BINDING, INTERACTION WITH ARPC2; CYFIP2; DLG4; GKAP1; HOMER1 AND SLC17A7.

    Entry informationi

    Entry nameiSHAN3_MOUSE
    AccessioniPrimary (citable) accession number: Q4ACU6
    Secondary accession number(s): F8S0X0
    , F8S0X2, F8S0X3, F8S0X5, F8S0X6, Q69ZD8, Q9JJZ3, S6BMD3, S6CCV8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 12, 2007
    Last sequence update: February 19, 2014
    Last modified: October 1, 2014
    This is version 91 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3