Q4AAX7 (TRUB_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase B
EC=5.4.99.25

Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase

Gene names

Name:	truB
Ordered Locus Names:	CBU_1430

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01080
Catalytic activity	tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01080
Sequence similarities	Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	tRNA pseudouridine synthase B HAMAP-Rule MF_01080		PRO_0000229352
Sites
	Active site	51	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4AAX7 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 2FD72193EDBB13D9	FASTA	309	34,689
	10 20 30 40 50 60 MTATNHPKLF KRPVDGVLLL DKPGGMTSNE ALQRVKRLFH AKKAGHTGSL DPLATGLLPI 70 80 90 100 110 120 CLGEATKFSQ FLLEADKSYS VKGRLGVRTA SGDSESPILT ERPIPKLTKR ALEKTLFAFR 130 140 150 160 170 180 GVIDQTPSMY SALKHKGQPL YKLARQGIEV ERKTRQVTIY ELTLLDWDNE SIELYVHCSK 190 200 210 220 230 240 GTYIRTLLDD VGEALGCGAH VVALRRLRVA HYHEDQMIKL AHLEREYDKA NYTGLDRYLL 250 260 270 280 290 300 PLETMVSHFP AIKLSSSTAF YLQQGQAVMV PNAPTHGFVR LRDQNDQFIG IGEILSDARI APRRLIQKR

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAZ22626.1.
RefSeq	YP_338283.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q4AAX7.
SMR	Q4AAX7. Positions 13-306.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1430.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ22626; AAZ22626; CBU_1430.
GeneID	1209336.
KEGG	cbu:CBU_1430.
PATRIC	17931611. VBICoxBur82552_1429.
Phylogenomic databases
eggNOG	COG0130.
HOGENOM	HOG000231223.
KO	K03177.
OMA	LGCGAYV.
ProtClustDB	PRK05033.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1417-MONOMER.
Family and domain databases
Gene3D	2.30.130.10. 1 hit.
HAMAP	MF_01080. TruB_bact.
InterPro	IPR002501. PsdUridine_synth. IPR020103. PsdUridine_synth_cat_dom. IPR015947. PUA-like_domain. IPR014780. tRNA_psdUridine_synth_TruB. IPR015240. tRNA_sdUridine_synth_fam1_C. [Graphical view]
PANTHER	PTHR13767. PTHR13767. 1 hit.
Pfam	PF09157. TruB-C_2. 1 hit. PF01509. TruB_N. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR00431. TruB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUB_COXBU

Accession

Primary (citable) accession number: Q4AAX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	September 13, 2005
Last modified:	May 29, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program