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Q4AAS1

- SYI_MYCHJ

UniProt

Q4AAS1 - SYI_MYCHJ

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Protein

Isoleucine--tRNA ligase

Gene
ileS, MHJ_0028
Organism
Mycoplasma hyopneumoniae (strain J / ATCC 25934 / NCTC 10110)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei546 – 5461Aminoacyl-adenylate By similarity
Binding sitei590 – 5901ATP By similarity
Metal bindingi856 – 8561Zinc By similarity
Metal bindingi859 – 8591Zinc By similarity
Metal bindingi870 – 8701Zinc By similarity
Metal bindingi873 – 8731Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMHYO262719:GJ59-28-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:MHJ_0028
OrganismiMycoplasma hyopneumoniae (strain J / ATCC 25934 / NCTC 10110)
Taxonomic identifieri262719 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000548: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 886886Isoleucine--tRNA ligaseUniRule annotationPRO_0000098420Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi262719.MHJ_0028.

Structurei

3D structure databases

ProteinModelPortaliQ4AAS1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotationAdd
BLAST
Motifi587 – 5915"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKESHFDE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4AAS1-1 [UniParc]FASTAAdd to Basket

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MDKNFYKNSL NIFNSNFSMK ANLSEKDKFY ADFWEKNQIY QQILRKRRGN    50
PRFILHDGPP YANGDIHIGH ALNKILKDII VRYKTMAGFY SPFVPGWDTH 100
GLPIENKIIN QIGSKSTLEI RRKSNDFANS QILAQMKQFK KLNLLTDFKQ 150
IYQTNTPNYE AKQLKLFKKM VSRGLVYRAL KPVYWSPSSQ SALAEAEIEY 200
LEYRSPSLFT SFDIKKGNNF VAENDKLIIW TTTPWTLIAN SGVAVGENFD 250
YVRIKNGENF YVLAANLLEK LAVIFDWKHY EIIDNFPGRA ILGIKYLHPI 300
FEKICPIVSG NHVSLDVGSG LVHLAPLFGE DDYWIGRENN LEMVMHVNDD 350
GKFNENAGQF SGQFYADSNK LITEFLEKKS KILHLSFIDH SFPHDWRTLK 400
PVIYRGTPQW FVSIEKIKKD LEKAIEEIEF PENWLKKRLT KMVVERKDWL 450
ISRQRSWGIP LIIFYDQNKD PVLDKPEIFD YIISLVEKFG SRIWYEKTTD 500
ELLPEKYQNL GWTKENDILD VWFDSGVSFF AANISDEKPP FDIYFEGSDQ 550
YRGWFNSSLI NSVIYFGFSP YKKLLSHGFV VDAKGNKMSK SRGNGVDPLL 600
ILSKYGCDIF RLWVANSEYY NDIVYSEAIF EQNVEIYRKI RNTVRFLITN 650
LADFKPTKYE LTEVDLYIFN KIQKLKNEII QNYDQNRFVR VVKIINNFII 700
EFSNFYLSIV KDILYADKKE SLKRRQVQYN LYELLQVLNI AIAPIMPTTA 750
EEIYSFIQKN NKQISVHMEE FFKESHFDEE LDAKWNEFFQ IKDSVYQLIE 800
QKIKSKEIKR PNEVGVLLKT DSDFIKSIDL KKLLMVAKVE FSNGKTEILQ 850
LNWEKCPRCW NHFEKINKVC ARCFEVLSEI VPEKNS 886
Length:886
Mass (Da):104,050
Last modified:May 29, 2007 - v2
Checksum:iCDCBAD01DD5853F7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017243 Genomic DNA. Translation: AAZ44122.2.
RefSeqiYP_278833.1. NC_007295.1.

Genome annotation databases

EnsemblBacteriaiAAZ44122; AAZ44122; MHJ_0028.
GeneIDi3563614.
KEGGimhj:MHJ_0028.
PATRICi20013758. VBIMycHyo90918_0029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017243 Genomic DNA. Translation: AAZ44122.2 .
RefSeqi YP_278833.1. NC_007295.1.

3D structure databases

ProteinModelPortali Q4AAS1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 262719.MHJ_0028.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ44122 ; AAZ44122 ; MHJ_0028 .
GeneIDi 3563614.
KEGGi mhj:MHJ_0028.
PATRICi 20013758. VBIMycHyo90918_0029.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KESHFDE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MHYO262719:GJ59-28-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Swine and poultry pathogens: the complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae."
    Vasconcelos A.T.R., Ferreira H.B., Bizarro C.V., Bonatto S.L., Carvalho M.O., Pinto P.M., Almeida D.F., Almeida L.G.P., Almeida R., Alves-Junior L., Assuncao E.N., Azevedo V.A.C., Bogo M.R., Brigido M.M., Brocchi M., Burity H.A., Camargo A.A., Camargo S.S.
    , Carepo M.S., Carraro D.M., de Mattos Cascardo J.C., Castro L.A., Cavalcanti G., Chemale G., Collevatti R.G., Cunha C.W., Dallagiovanna B., Dambros B.P., Dellagostin O.A., Falcao C., Fantinatti-Garboggini F., Felipe M.S.S., Fiorentin L., Franco G.R., Freitas N.S.A., Frias D., Grangeiro T.B., Grisard E.C., Guimaraes C.T., Hungria M., Jardim S.N., Krieger M.A., Laurino J.P., Lima L.F.A., Lopes M.I., Loreto E.L.S., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martinkovics C.T., Medeiros S.R.B., Moreira M.A.M., Neiva M., Ramalho-Neto C.E., Nicolas M.F., Oliveira S.C., Paixao R.F.C., Pedrosa F.O., Pena S.D.J., Pereira M., Pereira-Ferrari L., Piffer I., Pinto L.S., Potrich D.P., Salim A.C.M., Santos F.R., Schmitt R., Schneider M.P.C., Schrank A., Schrank I.S., Schuck A.F., Seuanez H.N., Silva D.W., Silva R., Silva S.C., Soares C.M.A., Souza K.R.L., Souza R.C., Staats C.C., Steffens M.B.R., Teixeira S.M.R., Urmenyi T.P., Vainstein M.H., Zuccherato L.W., Simpson A.J.G., Zaha A.
    J. Bacteriol. 187:5568-5577(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J / ATCC 25934 / NCTC 10110.

Entry informationi

Entry nameiSYI_MYCHJ
AccessioniPrimary (citable) accession number: Q4AAS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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