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Q4AAS1

- SYI_MYCHJ

UniProt

Q4AAS1 - SYI_MYCHJ

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma hyopneumoniae (strain J / ATCC 25934 / NCTC 10110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei546 – 5461Aminoacyl-adenylateUniRule annotation
Binding sitei590 – 5901ATPUniRule annotation
Metal bindingi856 – 8561ZincUniRule annotation
Metal bindingi859 – 8591ZincUniRule annotation
Metal bindingi870 – 8701ZincUniRule annotation
Metal bindingi873 – 8731ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMHYO262719:GJ59-28-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MHJ_0028
OrganismiMycoplasma hyopneumoniae (strain J / ATCC 25934 / NCTC 10110)
Taxonomic identifieri262719 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000548: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 886886Isoleucine--tRNA ligasePRO_0000098420Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi262719.MHJ_0028.

Structurei

3D structure databases

ProteinModelPortaliQ4AAS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi587 – 5915"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKESHFDE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4AAS1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDKNFYKNSL NIFNSNFSMK ANLSEKDKFY ADFWEKNQIY QQILRKRRGN
60 70 80 90 100
PRFILHDGPP YANGDIHIGH ALNKILKDII VRYKTMAGFY SPFVPGWDTH
110 120 130 140 150
GLPIENKIIN QIGSKSTLEI RRKSNDFANS QILAQMKQFK KLNLLTDFKQ
160 170 180 190 200
IYQTNTPNYE AKQLKLFKKM VSRGLVYRAL KPVYWSPSSQ SALAEAEIEY
210 220 230 240 250
LEYRSPSLFT SFDIKKGNNF VAENDKLIIW TTTPWTLIAN SGVAVGENFD
260 270 280 290 300
YVRIKNGENF YVLAANLLEK LAVIFDWKHY EIIDNFPGRA ILGIKYLHPI
310 320 330 340 350
FEKICPIVSG NHVSLDVGSG LVHLAPLFGE DDYWIGRENN LEMVMHVNDD
360 370 380 390 400
GKFNENAGQF SGQFYADSNK LITEFLEKKS KILHLSFIDH SFPHDWRTLK
410 420 430 440 450
PVIYRGTPQW FVSIEKIKKD LEKAIEEIEF PENWLKKRLT KMVVERKDWL
460 470 480 490 500
ISRQRSWGIP LIIFYDQNKD PVLDKPEIFD YIISLVEKFG SRIWYEKTTD
510 520 530 540 550
ELLPEKYQNL GWTKENDILD VWFDSGVSFF AANISDEKPP FDIYFEGSDQ
560 570 580 590 600
YRGWFNSSLI NSVIYFGFSP YKKLLSHGFV VDAKGNKMSK SRGNGVDPLL
610 620 630 640 650
ILSKYGCDIF RLWVANSEYY NDIVYSEAIF EQNVEIYRKI RNTVRFLITN
660 670 680 690 700
LADFKPTKYE LTEVDLYIFN KIQKLKNEII QNYDQNRFVR VVKIINNFII
710 720 730 740 750
EFSNFYLSIV KDILYADKKE SLKRRQVQYN LYELLQVLNI AIAPIMPTTA
760 770 780 790 800
EEIYSFIQKN NKQISVHMEE FFKESHFDEE LDAKWNEFFQ IKDSVYQLIE
810 820 830 840 850
QKIKSKEIKR PNEVGVLLKT DSDFIKSIDL KKLLMVAKVE FSNGKTEILQ
860 870 880
LNWEKCPRCW NHFEKINKVC ARCFEVLSEI VPEKNS
Length:886
Mass (Da):104,050
Last modified:May 29, 2007 - v2
Checksum:iCDCBAD01DD5853F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017243 Genomic DNA. Translation: AAZ44122.2.
RefSeqiYP_278833.1. NC_007295.1.

Genome annotation databases

EnsemblBacteriaiAAZ44122; AAZ44122; MHJ_0028.
GeneIDi3563614.
KEGGimhj:MHJ_0028.
PATRICi20013758. VBIMycHyo90918_0029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017243 Genomic DNA. Translation: AAZ44122.2 .
RefSeqi YP_278833.1. NC_007295.1.

3D structure databases

ProteinModelPortali Q4AAS1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 262719.MHJ_0028.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ44122 ; AAZ44122 ; MHJ_0028 .
GeneIDi 3563614.
KEGGi mhj:MHJ_0028.
PATRICi 20013758. VBIMycHyo90918_0029.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KESHFDE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MHYO262719:GJ59-28-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Swine and poultry pathogens: the complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae."
    Vasconcelos A.T.R., Ferreira H.B., Bizarro C.V., Bonatto S.L., Carvalho M.O., Pinto P.M., Almeida D.F., Almeida L.G.P., Almeida R., Alves-Junior L., Assuncao E.N., Azevedo V.A.C., Bogo M.R., Brigido M.M., Brocchi M., Burity H.A., Camargo A.A., Camargo S.S.
    , Carepo M.S., Carraro D.M., de Mattos Cascardo J.C., Castro L.A., Cavalcanti G., Chemale G., Collevatti R.G., Cunha C.W., Dallagiovanna B., Dambros B.P., Dellagostin O.A., Falcao C., Fantinatti-Garboggini F., Felipe M.S.S., Fiorentin L., Franco G.R., Freitas N.S.A., Frias D., Grangeiro T.B., Grisard E.C., Guimaraes C.T., Hungria M., Jardim S.N., Krieger M.A., Laurino J.P., Lima L.F.A., Lopes M.I., Loreto E.L.S., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martinkovics C.T., Medeiros S.R.B., Moreira M.A.M., Neiva M., Ramalho-Neto C.E., Nicolas M.F., Oliveira S.C., Paixao R.F.C., Pedrosa F.O., Pena S.D.J., Pereira M., Pereira-Ferrari L., Piffer I., Pinto L.S., Potrich D.P., Salim A.C.M., Santos F.R., Schmitt R., Schneider M.P.C., Schrank A., Schrank I.S., Schuck A.F., Seuanez H.N., Silva D.W., Silva R., Silva S.C., Soares C.M.A., Souza K.R.L., Souza R.C., Staats C.C., Steffens M.B.R., Teixeira S.M.R., Urmenyi T.P., Vainstein M.H., Zuccherato L.W., Simpson A.J.G., Zaha A.
    J. Bacteriol. 187:5568-5577(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J / ATCC 25934 / NCTC 10110.

Entry informationi

Entry nameiSYI_MYCHJ
AccessioniPrimary (citable) accession number: Q4AAS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: May 29, 2007
Last modified: November 26, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3