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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Mycoplasma hyopneumoniae (strain J / ATCC 25934 / NCTC 10110)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei253ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMHYO262719:G1G0R-158-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:MHJ_0137
OrganismiMycoplasma hyopneumoniae (strain J / ATCC 25934 / NCTC 10110)
Taxonomic identifieri262719 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000548 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002373731 – 467Glutamate--tRNA ligaseAdd BLAST467

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi262719.MHJ_0137

Structurei

3D structure databases

ProteinModelPortaliQ4AAJ3
SMRiQ4AAJ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionUniRule annotationAdd BLAST11
Motifi250 – 254"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiHYINTLP

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q4AAJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIRTRYAPS PTGFLHIGGA RTALFNYLFA KHHNGDFILR IEDSDNSRNI
60 70 80 90 100
KDGEKSQIEN LLWLGIIPDE KPGSETKFGP YRQSEKLERY QKLAQELIKK
110 120 130 140 150
GFAYYAFDNQ EELKLQKKEQ IAKGIFSFRY DQNWLKISDQ EKQKRLKNKH
160 170 180 190 200
FVIRFKVDKA KNFCWNDLVR GQICFEGSAI SDWVIIKSDG FPTYNFAVVV
210 220 230 240 250
DDFDMEISHI FRGEEHISNT PKQIGIYQAF NWKTPKFGHL TIITDKNGKK
260 270 280 290 300
LSKRDKNLFQ FIEDYKNQGY HSEAFFNFLA LLGWTSPDSQ EFFDHKSLIK
310 320 330 340 350
AFDYKRLSKA PSHFDIEKLN WFSKSYISKM PVDKILENLE LSDNQIWNRF
360 370 380 390 400
FVETFQKSSI KYADFYKNFE FFHRPKQEMD EKMLEIFEKL DKKPVKIFAS
410 420 430 440 450
KIDYQNWDYT KINDLIKEIG QKLEITGKNL LLPIRLATTF TNSGPELARA
460
IWLLGKKIIE KRLLKWK
Length:467
Mass (Da):55,311
Last modified:May 29, 2007 - v2
Checksum:iE85F512E16BAD9D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017243 Genomic DNA Translation: AAZ44228.2
RefSeqiWP_044284591.1, NC_007295.1

Genome annotation databases

EnsemblBacteriaiAAZ44228; AAZ44228; MHJ_0137
KEGGimhj:MHJ_0137

Similar proteinsi

Entry informationi

Entry nameiSYE_MYCHJ
AccessioniPrimary (citable) accession number: Q4AAJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 29, 2007
Last modified: March 28, 2018
This is version 78 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health