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Q4A8Y0

- SYI_MYCH7

UniProt

Q4A8Y0 - SYI_MYCH7

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Protein
Isoleucine--tRNA ligase
Gene
ileS, MHP7448_0032
Organism
Mycoplasma hyopneumoniae (strain 7448)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei546 – 5461Aminoacyl-adenylate By similarity
Binding sitei590 – 5901ATP By similarity
Metal bindingi856 – 8561Zinc By similarity
Metal bindingi859 – 8591Zinc By similarity
Metal bindingi870 – 8701Zinc By similarity
Metal bindingi873 – 8731Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMHYO262722:GHZR-32-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:MHP7448_0032
OrganismiMycoplasma hyopneumoniae (strain 7448)
Taxonomic identifieri262722 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000553: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 886886Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098419Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi262722.MHP7448_0032.

Structurei

3D structure databases

ProteinModelPortaliQ4A8Y0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotation
Add
BLAST
Motifi587 – 5915"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKESHFDE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4A8Y0-1 [UniParc]FASTAAdd to Basket

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MDKNFYKNSL NIFNSNFSMK ANLSEKDKFY ADFWEKNQIY QQILRKRRGN    50
PRFILHDGPP YANGDIHIGH ALNKILKDII VRYKTMAGFY SPFVPGWDTH 100
GLPIENKIIN QIGSKSTLEI RRKSNDFANS QILAQMAQFK KLNLLTDFKQ 150
IYQTNTPNYE AKQLKLFKKM VSRGLVYRAL KPVYWSPSSQ SALAEAEIEY 200
LEYRSPSLFT SFDIKKGNNF VAENDKLIIW TTTPWTLIAN SGVAVGENFD 250
YVRIKNEENF YVLAANLLEK LAAIFDWKHY EIIDNFPGKS ILGIKYLHPI 300
FEKICPIVSG NHVSLDVGSG LVHLAPLFGE DDYWIGRENN LEMVMHVNDD 350
GKFNENAGQF SGQFYANSNK LITEFLEKKS KILHLSFIDH SFPHDWRTLK 400
PVIYRGTPQW FVSIEKIKKD LEKAIEEIEF PENWLKKRLT KMVVERKDWL 450
ISRQRSWGIP LIIFYDQNKE PVLDKPEIFD YIISLVEKFG SRIWYEKTTD 500
ELLPEKYQNL GWTKENDILD VWFDSGVSFF AANISDEKPP FDIYFEGSDQ 550
YRGWFNSSLI NSVIYFGFSP YKKLLSHGFV VDAKGNKMSK SRGNGVDPLV 600
ILSKYGCDIF RLWVANSEYY NDIVYSEAIF EQNVEIYRKI RNTVRFLITN 650
LADFKPKKYE LTEVDLYIFN KIQKLKNEII QNYDQNRFVR VVKIINNFII 700
EFSNFYLSIV KDILYADKKE SLKRRQVQYN LYELLQVLNI AIAPIMPTTA 750
EEIYSFIQKN NKQISVHMEE FFKESHFDEE LVAKWDEFFQ IKDSVYQLIE 800
QKIKSKEIKR PNEVGVLLKT DSDFIKSIDL EKLLMVAKVE FSNEKTEILQ 850
LNWEKCPRCW NHFEKINKVC ARCFEVLSEI VPEKNS 886
Length:886
Mass (Da):104,109
Last modified:May 29, 2007 - v2
Checksum:i1CFF2F4FADEE3977
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017244 Genomic DNA. Translation: AAZ53409.2.
RefSeqiYP_287432.1. NC_007332.1.

Genome annotation databases

EnsemblBacteriaiAAZ53409; AAZ53409; MHP7448_0032.
GeneIDi3570990.
KEGGimhp:MHP7448_0032.
PATRICi20012166. VBIMycHyo9249_0036.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017244 Genomic DNA. Translation: AAZ53409.2 .
RefSeqi YP_287432.1. NC_007332.1.

3D structure databases

ProteinModelPortali Q4A8Y0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 262722.MHP7448_0032.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ53409 ; AAZ53409 ; MHP7448_0032 .
GeneIDi 3570990.
KEGGi mhp:MHP7448_0032.
PATRICi 20012166. VBIMycHyo9249_0036.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KESHFDE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MHYO262722:GHZR-32-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Swine and poultry pathogens: the complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae."
    Vasconcelos A.T.R., Ferreira H.B., Bizarro C.V., Bonatto S.L., Carvalho M.O., Pinto P.M., Almeida D.F., Almeida L.G.P., Almeida R., Alves-Junior L., Assuncao E.N., Azevedo V.A.C., Bogo M.R., Brigido M.M., Brocchi M., Burity H.A., Camargo A.A., Camargo S.S.
    , Carepo M.S., Carraro D.M., de Mattos Cascardo J.C., Castro L.A., Cavalcanti G., Chemale G., Collevatti R.G., Cunha C.W., Dallagiovanna B., Dambros B.P., Dellagostin O.A., Falcao C., Fantinatti-Garboggini F., Felipe M.S.S., Fiorentin L., Franco G.R., Freitas N.S.A., Frias D., Grangeiro T.B., Grisard E.C., Guimaraes C.T., Hungria M., Jardim S.N., Krieger M.A., Laurino J.P., Lima L.F.A., Lopes M.I., Loreto E.L.S., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martinkovics C.T., Medeiros S.R.B., Moreira M.A.M., Neiva M., Ramalho-Neto C.E., Nicolas M.F., Oliveira S.C., Paixao R.F.C., Pedrosa F.O., Pena S.D.J., Pereira M., Pereira-Ferrari L., Piffer I., Pinto L.S., Potrich D.P., Salim A.C.M., Santos F.R., Schmitt R., Schneider M.P.C., Schrank A., Schrank I.S., Schuck A.F., Seuanez H.N., Silva D.W., Silva R., Silva S.C., Soares C.M.A., Souza K.R.L., Souza R.C., Staats C.C., Steffens M.B.R., Teixeira S.M.R., Urmenyi T.P., Vainstein M.H., Zuccherato L.W., Simpson A.J.G., Zaha A.
    J. Bacteriol. 187:5568-5577(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 7448.

Entry informationi

Entry nameiSYI_MYCH7
AccessioniPrimary (citable) accession number: Q4A8Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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