Q4A5N2 (Q4A5N2_MYCS5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL AAZ43939.1
Ordered Locus Names:	MS53_0531

Organism

Mycoplasma synoviae (strain 53) [Complete proteome] [HAMAP]

Taxonomic identifier

262723 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q4A5N2 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 5EAE7B172030C75A	FASTA	625	72,153
	10 20 30 40 50 60 MNKLVIVESP NKVKTIQKYL GNDYNVISCV GHIYKMKTSG PFGLGIDFEN WEPLYEFDST 70 80 90 100 110 120 KKKVGKELKD SLKKAQMVYI ATDPDREGES IGENLVSYLK LNDNYKRVTY NEITKDAILK 130 140 150 160 170 180 AFDNPHDLNW ALINAQKSRR MLDRIIGFRL SKLLQSKLQN APSNASAGRV QSIALKLVID 190 200 210 220 230 240 REREILAFVP TKYWKLNAFL LNENSLKAQY FNEENSSGQK EWIFESQIKE VKEFFEKLKD 250 260 270 280 290 300 KKVKVTKINV TEKKNKALVP FKQAAIYKRS PYSAAMTQST LQKLYEGYGD GGLISYPRTD 310 320 330 340 350 360 STRLSQTFVN QAKKFIESKY GSDFVSSEVK GFTGDQDAHE AIRPTDLNLL PENLEKLYPT 370 380 390 400 410 420 ISSNEVNLYK LIYEHTLKAL IKPPVIKSKQ YIYQVDKYLF KNSFATVTFQ GYYCVSAPEE 430 440 450 460 470 480 KQEKDPNFEI NQEVTVNNFA FEDLETKPTP RYNDGSLIEA LDDIKVGRPS TFASTVKIIK 490 500 510 520 530 540 DRQYVEVLDS NAIKPTDFGM IVLDKLVNNF PEIINEKYTA EVEGQLDLIS ENKLEKNQVM 550 560 570 580 590 600 HDFYTKFNEL YDQVASTMEK TRLEDNFLDE DCPDCSGKLL IRLNRKKQKF IGCKSFPNCT 610 620 YTRSYEDPDA KKRYYRNYKN TNKSN

References

[1]

"Swine and poultry pathogens: the complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae." Vasconcelos A.T.R., Ferreira H.B., Bizarro C.V., Bonatto S.L., Carvalho M.O., Pinto P.M., Almeida D.F., Almeida L.G.P., Almeida R., Alves-Junior L., Assuncao E.N., Azevedo V.A.C., Bogo M.R., Brigido M.M., Brocchi M., Burity H.A., Camargo A.A., Camargo S.S. , Carepo M.S., Carraro D.M., de Mattos Cascardo J.C., Castro L.A., Cavalcanti G., Chemale G., Collevatti R.G., Cunha C.W., Dallagiovanna B., Dambros B.P., Dellagostin O.A., Falcao C., Fantinatti-Garboggini F., Felipe M.S.S., Fiorentin L., Franco G.R., Freitas N.S.A., Frias D., Grangeiro T.B., Grisard E.C., Guimaraes C.T., Hungria M., Jardim S.N., Krieger M.A., Laurino J.P., Lima L.F.A., Lopes M.I., Loreto E.L.S., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martinkovics C.T., Medeiros S.R.B., Moreira M.A.M., Neiva M., Ramalho-Neto C.E., Nicolas M.F., Oliveira S.C., Paixao R.F.C., Pedrosa F.O., Pena S.D.J., Pereira M., Pereira-Ferrari L., Piffer I., Pinto L.S., Potrich D.P., Salim A.C.M., Santos F.R., Schmitt R., Schneider M.P.C., Schrank A., Schrank I.S., Schuck A.F., Seuanez H.N., Silva D.W., Silva R., Silva S.C., Soares C.M.A., Souza K.R.L., Souza R.C., Staats C.C., Steffens M.B.R., Teixeira S.M.R., Urmenyi T.P., Vainstein M.H., Zuccherato L.W., Simpson A.J.G., Zaha A. J. Bacteriol. 187:5568-5577(2005) [PubMed: 16077101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017245 Genomic DNA. Translation: AAZ43939.1.
RefSeq	YP_278650.1. NC_007294.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4A5N2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3564566.
GenomeReviews	Gene locus MS53_0531 in contig AE017245_GR.
KEGG	msy:MS53_0531.
PATRIC	20025704. VBIMycSyn118523_0567.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	TIEISIM.
PhylomeDB	Q4A5N2.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q4A5N2_MYCS5

Accession

Primary (citable) accession number: Q4A5N2

Entry history

Integrated into UniProtKB/TrEMBL:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)