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Q4A5K5

- SYI_MYCS5

UniProt

Q4A5K5 - SYI_MYCS5

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma synoviae (strain 53)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 2 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
    Binding sitei597 – 5971ATPUniRule annotation
    Metal bindingi866 – 8661ZincUniRule annotation
    Metal bindingi869 – 8691ZincUniRule annotation
    Metal bindingi883 – 8831ZincUniRule annotation
    Metal bindingi886 – 8861ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMSYN262723:GH37-572-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MS53_0558
    OrganismiMycoplasma synoviae (strain 53)
    Taxonomic identifieri262723 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000549: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 897897Isoleucine--tRNA ligasePRO_0000098426Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi262723.MS53_0558.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4A5K5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4A5K5-1 [UniParc]FASTAAdd to Basket

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    MSLDFKKTLN MPSTKFDMKA NLVEKEPLFR KKWLEDDIYQ KVLKNNANNE    50
    RFVVHDGPPY ANGDIHVGHA LNKILKDIIV RYKSLQGYYS PFVPGWDTHG 100
    LPIEHKMLTE SKLDRDQITV ELLRKKSRNY ALKQIEHQKK QFQKLQLFSD 150
    FSKIYITLDK SYEAKQLKVF KKLALDGLVY KGLKPIYWSP SSQSALAEAE 200
    VEYETVTTNS IYVSFDVTKS TFNKVPVGSK LVVWTTTPWT LIANAAVAIS 250
    FDITYLTVKY QDSLYVVAKN LFYNDLLEKF QWENYEVVDE FLGKEMPRNS 300
    IWYKAPLLDF DAPVIATNYV LEDSGTGLVH SAPLFGEDDF SLTFDNDLKL 350
    IMHISDTGHI ENSQTKYDSL FYEEANKEII KDLAEKVVHV YTYSHSYPHD 400
    WRTKKPIIYR ATPQWFVSID KVRSKIVSEL QNKVKTFPEW SKNRMISMIE 450
    NRGDWTISRQ RTWGVPIIIF YDENEKPVIN EEIFDHVIDL VANHGTDIWF 500
    SSTVDELLPE KYRNRNWTKE NDIMDVWFDS GVSSIAVDID GGKTTLPFDV 550
    YLEGNDQFRG WFNSSVINAV AYAGVSPYIN LVSHGFALDG QGKKMSKSRN 600
    NVVDPLDVIK KYGADILRLW VANSEYSSDV HISESILVQN SEIYRKIRNT 650
    VKFLLGNLNN FKYDKDLKLT SIHHYINEEL KSVKKEVLEN YDKFRFINVI 700
    KVLNRYVIDL SSFYFSVTKD ILYIRKENDE ERQMVLKNFY EILDFLMLAL 750
    APIIPTTADE MYSYFNKENK KESLFLERLE KAGDVSFDEK VLEQFKEFFE 800
    LRDQVNILIE NQIQNKVIKR SNELELVLPE TASEFLKSLD LKTLLMVSKI 850
    SYGKTLQVVK FESEKCKRCW NHFASLNKEY EICDLCFSVL KDTLANA 897
    Length:897
    Mass (Da):104,509
    Last modified:June 12, 2007 - v2
    Checksum:iA74A22BE93E9AF17
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017245 Genomic DNA. Translation: AAZ43966.2.
    RefSeqiYP_278677.1. NC_007294.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ43966; AAZ43966; MS53_0558.
    GeneIDi3564027.
    KEGGimsy:MS53_0558.
    PATRICi20025766. VBIMycSyn118523_0598.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017245 Genomic DNA. Translation: AAZ43966.2 .
    RefSeqi YP_278677.1. NC_007294.1.

    3D structure databases

    ProteinModelPortali Q4A5K5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 262723.MS53_0558.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ43966 ; AAZ43966 ; MS53_0558 .
    GeneIDi 3564027.
    KEGGi msy:MS53_0558.
    PATRICi 20025766. VBIMycSyn118523_0598.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci MSYN262723:GH37-572-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Swine and poultry pathogens: the complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae."
      Vasconcelos A.T.R., Ferreira H.B., Bizarro C.V., Bonatto S.L., Carvalho M.O., Pinto P.M., Almeida D.F., Almeida L.G.P., Almeida R., Alves-Junior L., Assuncao E.N., Azevedo V.A.C., Bogo M.R., Brigido M.M., Brocchi M., Burity H.A., Camargo A.A., Camargo S.S.
      , Carepo M.S., Carraro D.M., de Mattos Cascardo J.C., Castro L.A., Cavalcanti G., Chemale G., Collevatti R.G., Cunha C.W., Dallagiovanna B., Dambros B.P., Dellagostin O.A., Falcao C., Fantinatti-Garboggini F., Felipe M.S.S., Fiorentin L., Franco G.R., Freitas N.S.A., Frias D., Grangeiro T.B., Grisard E.C., Guimaraes C.T., Hungria M., Jardim S.N., Krieger M.A., Laurino J.P., Lima L.F.A., Lopes M.I., Loreto E.L.S., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martinkovics C.T., Medeiros S.R.B., Moreira M.A.M., Neiva M., Ramalho-Neto C.E., Nicolas M.F., Oliveira S.C., Paixao R.F.C., Pedrosa F.O., Pena S.D.J., Pereira M., Pereira-Ferrari L., Piffer I., Pinto L.S., Potrich D.P., Salim A.C.M., Santos F.R., Schmitt R., Schneider M.P.C., Schrank A., Schrank I.S., Schuck A.F., Seuanez H.N., Silva D.W., Silva R., Silva S.C., Soares C.M.A., Souza K.R.L., Souza R.C., Staats C.C., Steffens M.B.R., Teixeira S.M.R., Urmenyi T.P., Vainstein M.H., Zuccherato L.W., Simpson A.J.G., Zaha A.
      J. Bacteriol. 187:5568-5577(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 53.

    Entry informationi

    Entry nameiSYI_MYCS5
    AccessioniPrimary (citable) accession number: Q4A5K5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 69 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3