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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma synoviae (strain 53)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
Binding sitei597 – 5971ATPUniRule annotation
Metal bindingi866 – 8661ZincUniRule annotation
Metal bindingi869 – 8691ZincUniRule annotation
Metal bindingi883 – 8831ZincUniRule annotation
Metal bindingi886 – 8861ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMSYN262723:GH37-572-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MS53_0558
OrganismiMycoplasma synoviae (strain 53)
Taxonomic identifieri262723 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000549 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 897897Isoleucine--tRNA ligasePRO_0000098426Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi262723.MS53_0558.

Structurei

3D structure databases

ProteinModelPortaliQ4A5K5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4A5K5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLDFKKTLN MPSTKFDMKA NLVEKEPLFR KKWLEDDIYQ KVLKNNANNE
60 70 80 90 100
RFVVHDGPPY ANGDIHVGHA LNKILKDIIV RYKSLQGYYS PFVPGWDTHG
110 120 130 140 150
LPIEHKMLTE SKLDRDQITV ELLRKKSRNY ALKQIEHQKK QFQKLQLFSD
160 170 180 190 200
FSKIYITLDK SYEAKQLKVF KKLALDGLVY KGLKPIYWSP SSQSALAEAE
210 220 230 240 250
VEYETVTTNS IYVSFDVTKS TFNKVPVGSK LVVWTTTPWT LIANAAVAIS
260 270 280 290 300
FDITYLTVKY QDSLYVVAKN LFYNDLLEKF QWENYEVVDE FLGKEMPRNS
310 320 330 340 350
IWYKAPLLDF DAPVIATNYV LEDSGTGLVH SAPLFGEDDF SLTFDNDLKL
360 370 380 390 400
IMHISDTGHI ENSQTKYDSL FYEEANKEII KDLAEKVVHV YTYSHSYPHD
410 420 430 440 450
WRTKKPIIYR ATPQWFVSID KVRSKIVSEL QNKVKTFPEW SKNRMISMIE
460 470 480 490 500
NRGDWTISRQ RTWGVPIIIF YDENEKPVIN EEIFDHVIDL VANHGTDIWF
510 520 530 540 550
SSTVDELLPE KYRNRNWTKE NDIMDVWFDS GVSSIAVDID GGKTTLPFDV
560 570 580 590 600
YLEGNDQFRG WFNSSVINAV AYAGVSPYIN LVSHGFALDG QGKKMSKSRN
610 620 630 640 650
NVVDPLDVIK KYGADILRLW VANSEYSSDV HISESILVQN SEIYRKIRNT
660 670 680 690 700
VKFLLGNLNN FKYDKDLKLT SIHHYINEEL KSVKKEVLEN YDKFRFINVI
710 720 730 740 750
KVLNRYVIDL SSFYFSVTKD ILYIRKENDE ERQMVLKNFY EILDFLMLAL
760 770 780 790 800
APIIPTTADE MYSYFNKENK KESLFLERLE KAGDVSFDEK VLEQFKEFFE
810 820 830 840 850
LRDQVNILIE NQIQNKVIKR SNELELVLPE TASEFLKSLD LKTLLMVSKI
860 870 880 890
SYGKTLQVVK FESEKCKRCW NHFASLNKEY EICDLCFSVL KDTLANA
Length:897
Mass (Da):104,509
Last modified:June 12, 2007 - v2
Checksum:iA74A22BE93E9AF17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017245 Genomic DNA. Translation: AAZ43966.2.
RefSeqiWP_041352082.1. NC_007294.1.

Genome annotation databases

EnsemblBacteriaiAAZ43966; AAZ43966; MS53_0558.
KEGGimsy:MS53_0558.
PATRICi20025766. VBIMycSyn118523_0598.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017245 Genomic DNA. Translation: AAZ43966.2.
RefSeqiWP_041352082.1. NC_007294.1.

3D structure databases

ProteinModelPortaliQ4A5K5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262723.MS53_0558.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ43966; AAZ43966; MS53_0558.
KEGGimsy:MS53_0558.
PATRICi20025766. VBIMycSyn118523_0598.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciMSYN262723:GH37-572-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Swine and poultry pathogens: the complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae."
    Vasconcelos A.T.R., Ferreira H.B., Bizarro C.V., Bonatto S.L., Carvalho M.O., Pinto P.M., Almeida D.F., Almeida L.G.P., Almeida R., Alves-Junior L., Assuncao E.N., Azevedo V.A.C., Bogo M.R., Brigido M.M., Brocchi M., Burity H.A., Camargo A.A., Camargo S.S.
    , Carepo M.S., Carraro D.M., de Mattos Cascardo J.C., Castro L.A., Cavalcanti G., Chemale G., Collevatti R.G., Cunha C.W., Dallagiovanna B., Dambros B.P., Dellagostin O.A., Falcao C., Fantinatti-Garboggini F., Felipe M.S.S., Fiorentin L., Franco G.R., Freitas N.S.A., Frias D., Grangeiro T.B., Grisard E.C., Guimaraes C.T., Hungria M., Jardim S.N., Krieger M.A., Laurino J.P., Lima L.F.A., Lopes M.I., Loreto E.L.S., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martinkovics C.T., Medeiros S.R.B., Moreira M.A.M., Neiva M., Ramalho-Neto C.E., Nicolas M.F., Oliveira S.C., Paixao R.F.C., Pedrosa F.O., Pena S.D.J., Pereira M., Pereira-Ferrari L., Piffer I., Pinto L.S., Potrich D.P., Salim A.C.M., Santos F.R., Schmitt R., Schneider M.P.C., Schrank A., Schrank I.S., Schuck A.F., Seuanez H.N., Silva D.W., Silva R., Silva S.C., Soares C.M.A., Souza K.R.L., Souza R.C., Staats C.C., Steffens M.B.R., Teixeira S.M.R., Urmenyi T.P., Vainstein M.H., Zuccherato L.W., Simpson A.J.G., Zaha A.
    J. Bacteriol. 187:5568-5577(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 53.

Entry informationi

Entry nameiSYI_MYCS5
AccessioniPrimary (citable) accession number: Q4A5K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 12, 2007
Last modified: July 22, 2015
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.