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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma synoviae (strain 53)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei553Aminoacyl-adenylateUniRule annotation1
Binding sitei597ATPUniRule annotation1
Metal bindingi866ZincUniRule annotation1
Metal bindingi869ZincUniRule annotation1
Metal bindingi883ZincUniRule annotation1
Metal bindingi886ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMSYN262723:G1G0T-582-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MS53_0558
OrganismiMycoplasma synoviae (strain 53)
Taxonomic identifieri262723 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000549 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984261 – 897Isoleucine--tRNA ligaseAdd BLAST897

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi262723.MS53_0558

Structurei

3D structure databases

ProteinModelPortaliQ4A5K5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 69"HIGH" regionAdd BLAST11
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q4A5K5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLDFKKTLN MPSTKFDMKA NLVEKEPLFR KKWLEDDIYQ KVLKNNANNE
60 70 80 90 100
RFVVHDGPPY ANGDIHVGHA LNKILKDIIV RYKSLQGYYS PFVPGWDTHG
110 120 130 140 150
LPIEHKMLTE SKLDRDQITV ELLRKKSRNY ALKQIEHQKK QFQKLQLFSD
160 170 180 190 200
FSKIYITLDK SYEAKQLKVF KKLALDGLVY KGLKPIYWSP SSQSALAEAE
210 220 230 240 250
VEYETVTTNS IYVSFDVTKS TFNKVPVGSK LVVWTTTPWT LIANAAVAIS
260 270 280 290 300
FDITYLTVKY QDSLYVVAKN LFYNDLLEKF QWENYEVVDE FLGKEMPRNS
310 320 330 340 350
IWYKAPLLDF DAPVIATNYV LEDSGTGLVH SAPLFGEDDF SLTFDNDLKL
360 370 380 390 400
IMHISDTGHI ENSQTKYDSL FYEEANKEII KDLAEKVVHV YTYSHSYPHD
410 420 430 440 450
WRTKKPIIYR ATPQWFVSID KVRSKIVSEL QNKVKTFPEW SKNRMISMIE
460 470 480 490 500
NRGDWTISRQ RTWGVPIIIF YDENEKPVIN EEIFDHVIDL VANHGTDIWF
510 520 530 540 550
SSTVDELLPE KYRNRNWTKE NDIMDVWFDS GVSSIAVDID GGKTTLPFDV
560 570 580 590 600
YLEGNDQFRG WFNSSVINAV AYAGVSPYIN LVSHGFALDG QGKKMSKSRN
610 620 630 640 650
NVVDPLDVIK KYGADILRLW VANSEYSSDV HISESILVQN SEIYRKIRNT
660 670 680 690 700
VKFLLGNLNN FKYDKDLKLT SIHHYINEEL KSVKKEVLEN YDKFRFINVI
710 720 730 740 750
KVLNRYVIDL SSFYFSVTKD ILYIRKENDE ERQMVLKNFY EILDFLMLAL
760 770 780 790 800
APIIPTTADE MYSYFNKENK KESLFLERLE KAGDVSFDEK VLEQFKEFFE
810 820 830 840 850
LRDQVNILIE NQIQNKVIKR SNELELVLPE TASEFLKSLD LKTLLMVSKI
860 870 880 890
SYGKTLQVVK FESEKCKRCW NHFASLNKEY EICDLCFSVL KDTLANA
Length:897
Mass (Da):104,509
Last modified:June 12, 2007 - v2
Checksum:iA74A22BE93E9AF17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017245 Genomic DNA Translation: AAZ43966.2
RefSeqiWP_041352082.1, NC_007294.1

Genome annotation databases

EnsemblBacteriaiAAZ43966; AAZ43966; MS53_0558
KEGGimsy:MS53_0558

Similar proteinsi

Entry informationi

Entry nameiSYI_MYCS5
AccessioniPrimary (citable) accession number: Q4A5K5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 12, 2007
Last modified: March 28, 2018
This is version 89 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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