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Protein

Beclin-1

Gene

BECN1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. May play a role in antiviral host defense (By similarity).By similarity
Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Apoptosis, Autophagy, Cell cycle, Cell division

Enzyme and pathway databases

ReactomeiR-BTA-1632852. Macroautophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Beclin-1
Cleaved into the following 2 chains:
Gene namesi
Name:BECN1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

  • Cytoplasm By similarity
  • Golgi apparatustrans-Golgi network membrane By similarity; Peripheral membrane protein By similarity
  • Endosome membrane By similarity; Peripheral membrane protein By similarity
  • Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity
  • Mitochondrion membrane By similarity; Peripheral membrane protein By similarity
  • Cytoplasmic vesicleautophagosome Curated

  • Note: Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.By similarity
Beclin-1-C 35 kDa :
  • Mitochondrion By similarity
  • Nucleus By similarity
  • Cytoplasm By similarity
Beclin-1-C 37 kDa :
  • Mitochondrion By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448Beclin-1PRO_0000244423Add
BLAST
Chaini132 – 448317Beclin-1-C 37 kDaBy similarityPRO_0000435034Add
BLAST
Chaini148 – 448301Beclin-1-C 35 kDaBy similarityPRO_0000435035Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei88 – 881Phosphoserine; by AMPKBy similarity
Modified residuei91 – 911Phosphoserine; by AMPKBy similarity
Modified residuei94 – 941Phosphoserine; by AMPKBy similarity
Modified residuei117 – 1171Phosphothreonine; by DAPK1By similarity

Post-translational modificationi

Phosphorylation at Thr-117 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy. In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy.By similarity
Polyubiquitinated by NEDD4, both with 'Lys11'- and 'Lys63'-linkages. 'Lys'-11-linked poyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes (By similarity).By similarity
Proteolytically processed by caspases including CASP8 and CASP3; the C-terminal fragments lack autophagy-inducing capacity and are proposed to induce apoptosis. Thus the cleavage is proposed to be an determinant to switch from autophagy to apoptosis pathways affecting cellular homeostasis including viral infections and survival of tumor cells.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ4A1L4.
PRIDEiQ4A1L4.

Interactioni

Subunit structurei

A homodimeric form is proposed to exist; this metastable form readily transits to ATG14- or UVRAG-containing complexes with BECN1:UVRAG being more stable than BECN1:ATG14 (By similarity). Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are PI3K complex I (PI3KC3-C1) containing ATG14, and PI3K complex II (PI3KC3-C2) containing UVRAG. PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex. Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits, such as RUBCN, SH3GLB1/Bif-1 and AMBRA1. PI3KC3-C1 probably associates with PIK3CB. Interacts with AMBRA1, GOPC, GRID2. Interacts with BCL2 and BCL2L1; the interaction inhibits BECN1 function in promoting autophagy by interfering with the formation of the PI3K complex. Interacts with cytosolic HMGB1; inhibits the interaction of BECN1 and BCL2 leading to promotion of autophagy. Interacts with USP10, USP13, VMP1, DAPK1, RAB39A. Interacts with SLAMF1. Interacts with TRIM5; the interaction causes activation of BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2. Interacts with active ULK1 (phosphorylated on 'Ser-317') and MEFV simultaneously (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Q8UZC13EBI-6874856,EBI-6878727From a different organism.

Protein-protein interaction databases

IntActiQ4A1L4. 1 interaction.
STRINGi9913.ENSBTAP00000026531.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni110 – 15748Interaction with BCL2By similarityAdd
BLAST
Regioni423 – 44826Required for membrane-associationBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili140 – 267128Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi106 – 12520BH3By similarityAdd
BLAST

Domaini

The coiled coil domain can form antiparallel homodimers and mediates dimerization with the coiled coil domains of ATG14 or UVRAG involved in the formation of PI3K complexes.By similarity

Sequence similaritiesi

Belongs to the beclin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2751. Eukaryota.
ENOG410XQ85. LUCA.
GeneTreeiENSGT00390000008164.
HOGENOMiHOG000158093.
HOVERGENiHBG003181.
InParanoidiQ4A1L4.
KOiK08334.
OMAiYIPPARM.
OrthoDBiEOG7D2FDG.
TreeFamiTF314282.

Family and domain databases

InterProiIPR007243. Atg6/Beclin.
IPR032913. BECN1.
IPR029318. BH3_dom.
[Graphical view]
PANTHERiPTHR12768. PTHR12768. 1 hit.
PTHR12768:SF6. PTHR12768:SF6. 1 hit.
PfamiPF04111. APG6. 1 hit.
PF15285. BH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4A1L4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGSKTSSST MQVSFVCQRC SQPLKLDTSF KILDRVTIQE LTAPLLATAQ
60 70 80 90 100
LKPGETQEEE ANSGEEPFIE TRQDGVSRRF IPPARMMSTE SANSFTLIGE
110 120 130 140 150
ASDGGTMENL SRRLKVTGDL FDIMSGQTDV DHPLCEECTD TLLDQLDTQL
160 170 180 190 200
NVTENECQNY KRCLEILEQM NEDDSEQLGL ELKELALEEE RLIQELEDVE
210 220 230 240 250
KNRKIVAENL EKVQAEAERL DQEEAQYQRE YSEFKRQQLE LDDELKSVEN
260 270 280 290 300
QMRYAQMQLD KLKKTNVFNA TFHIWHSGQF GTINNFRLGR LPSVPVEWNE
310 320 330 340 350
INAAWGQTVL LLHALANKMG LKFQRYRLVP YGNHSYLESL TDKSKELPLY
360 370 380 390 400
CSGGLRFFWD NKFDHAMVAF LDCVQQFKEE VEKGETRFCL PYRMDVEKGK
410 420 430 440
IEDTGGSGGS YSIKTQFNSE EQWTKALKFM LTNLKWGLAW VSSQFYNK
Length:448
Mass (Da):51,581
Last modified:September 13, 2005 - v1
Checksum:iF2B86F42D8510855
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM051355 mRNA. Translation: CAJ19744.1.
BC102177 mRNA. Translation: AAI02178.1.
RefSeqiNP_001028799.1. NM_001033627.2.
UniGeneiBt.106861.

Genome annotation databases

EnsembliENSBTAT00000026531; ENSBTAP00000026531; ENSBTAG00000019914.
GeneIDi527278.
KEGGibta:527278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM051355 mRNA. Translation: CAJ19744.1.
BC102177 mRNA. Translation: AAI02178.1.
RefSeqiNP_001028799.1. NM_001033627.2.
UniGeneiBt.106861.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ4A1L4. 1 interaction.
STRINGi9913.ENSBTAP00000026531.

Proteomic databases

PaxDbiQ4A1L4.
PRIDEiQ4A1L4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000026531; ENSBTAP00000026531; ENSBTAG00000019914.
GeneIDi527278.
KEGGibta:527278.

Organism-specific databases

CTDi8678.

Phylogenomic databases

eggNOGiKOG2751. Eukaryota.
ENOG410XQ85. LUCA.
GeneTreeiENSGT00390000008164.
HOGENOMiHOG000158093.
HOVERGENiHBG003181.
InParanoidiQ4A1L4.
KOiK08334.
OMAiYIPPARM.
OrthoDBiEOG7D2FDG.
TreeFamiTF314282.

Enzyme and pathway databases

ReactomeiR-BTA-1632852. Macroautophagy.

Miscellaneous databases

NextBioi20874552.

Family and domain databases

InterProiIPR007243. Atg6/Beclin.
IPR032913. BECN1.
IPR029318. BH3_dom.
[Graphical view]
PANTHERiPTHR12768. PTHR12768. 1 hit.
PTHR12768:SF6. PTHR12768:SF6. 1 hit.
PfamiPF04111. APG6. 1 hit.
PF15285. BH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Phylogeny and biochemistry of the autophagy protein beclin 1."
    Botti J., Djavaheri-Mergny M., Codogno P., Oriol R.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiBECN1_BOVIN
AccessioniPrimary (citable) accession number: Q4A1L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: September 13, 2005
Last modified: March 16, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.