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Q4A127

- HSDR_STAS1

UniProt

Q4A127 - HSDR_STAS1

Protein

Type-1 restriction enzyme R protein

Gene

hsdR

Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Subunit R is required for both nuclease and ATPase activities, but not for modification.By similarity

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi268 – 2747ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. Type I site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSSAP342451:GKFA-56-MONOMER.

    Protein family/group databases

    REBASEi11264. SsaAORF53P.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-1 restriction enzyme R protein (EC:3.1.21.3)
    Alternative name(s):
    Type I restriction enzyme R protein
    Gene namesi
    Name:hsdR
    Ordered Locus Names:SSP0054
    OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
    Taxonomic identifieri342451 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000006371: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 930930Type-1 restriction enzyme R proteinPRO_0000077272Add
    BLAST

    Interactioni

    Subunit structurei

    The type I restriction/modification system is composed of three polypeptides R, M and S.By similarity

    Protein-protein interaction databases

    STRINGi342451.SSP0054.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4A127.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini254 – 418165Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the HsdR family.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0610.
    HOGENOMiHOG000003518.
    KOiK01153.
    OMAiNNGYIWH.
    OrthoDBiEOG6JTCB4.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR027417. P-loop_NTPase.
    IPR007409. Restrct_endonuc_type1_HsdR_N.
    IPR004473. Restrct_endonuc_typeI_HsdR.
    IPR022625. TypeI_RM_Rsu_C.
    [Graphical view]
    PfamiPF12008. EcoR124_C. 1 hit.
    PF04313. HSDR_N. 1 hit.
    PF04851. ResIII. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00348. hsdR. 1 hit.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4A127-1 [UniParc]FASTAAdd to Basket

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    MVYQSEFALE TEMMEQLKSN GYETVTIRNE QQLLDNFRSI LNERHADKLN    50
    GDPLTDKEFQ RLLTMINGKG IFESARILRD KMPLKRDDES EVYLSFLDTR 100
    HWCQNKFQIT NQVSVDDTYK ARYDVTILIN GLPLVQIELK RRGIDINEAF 150
    NQVMRYRKQN YTGLFRYIQL FIISNGIDTR YISNNDGEIY KSHMFYWSDK 200
    ENNRINTLNE FTETFLRPCH IAKMISRYMI LNETDNILMA MRPYQVHAVE 250
    ALIHQATETS NNGYIWHTTG SGKTLTSFKA SQVLSEQDDI KKVIFLVDRK 300
    DLDSQTEEEF NKFSKGSVDK TNNTAQLVKQ LKDKSLPLIV TTIQKMSKAI 350
    QNNAEALDQY KTDKVVFIID ECHRSQFGDM HRIVRQHFNN AQYFGFTGTP 400
    RFEENQSQDG RSTADIFGRC LHTYLIKDAI HDGNVLGFSV DYINTIKAQN 450
    IDTETDELVE GINTDEVWLS DQRVELIARH ITENHDKYTR NRQYSAIFTV 500
    QSIPALVKYY DAFKKISKDY EHPLKVAGVF SYAANEERNE GEVDEAHSRG 550
    KLEEVIQDYN LNFGTNFSTD TFQEYFNHIS KNVKKGVKDN KIDVLIVVNM 600
    FLTGFDSKVL NTLYVDKNLK YHDLIQAYSR TNRVEKETKP FGKIVNYRDL 650
    KQNTDNALKL FSQTEDTDRV LMRDYDEYKA EFVDALAELK AVALKPQDMD 700
    QVQDENEKKA FVEAFRLVSK LVLRLKAFDE FDFTKANIGM NEQEFEDYKS 750
    KYFAIYDEVK PKRGEVEKVS ILNDIDFEIE ILRNDRINVS YIMDLVRQID 800
    LKDKAEQQRN RDQIRRMLDN ADDPTLRLKR DLLREFIDDV IPELSEEDNI 850
    DEAYILFENA KRESEFNQFA QQQAVDEQVL KDITGEYEYS GIVNQDHLKE 900
    LVGDKKLREK RQTKKAVTSF VEEVSEKYSS 930
    Length:930
    Mass (Da):108,873
    Last modified:September 13, 2005 - v1
    Checksum:i559FA69A2AC96E24
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008934 Genomic DNA. Translation: BAE17199.1.
    RefSeqiYP_300144.1. NC_007350.1.

    Genome annotation databases

    EnsemblBacteriaiBAE17199; BAE17199; SSP0054.
    GeneIDi3617259.
    KEGGissp:SSP0054.
    PATRICi19621587. VBIStaSap90642_0054.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008934 Genomic DNA. Translation: BAE17199.1 .
    RefSeqi YP_300144.1. NC_007350.1.

    3D structure databases

    ProteinModelPortali Q4A127.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 342451.SSP0054.

    Protein family/group databases

    REBASEi 11264. SsaAORF53P.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAE17199 ; BAE17199 ; SSP0054 .
    GeneIDi 3617259.
    KEGGi ssp:SSP0054.
    PATRICi 19621587. VBIStaSap90642_0054.

    Phylogenomic databases

    eggNOGi COG0610.
    HOGENOMi HOG000003518.
    KOi K01153.
    OMAi NNGYIWH.
    OrthoDBi EOG6JTCB4.

    Enzyme and pathway databases

    BioCyci SSAP342451:GKFA-56-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR027417. P-loop_NTPase.
    IPR007409. Restrct_endonuc_type1_HsdR_N.
    IPR004473. Restrct_endonuc_typeI_HsdR.
    IPR022625. TypeI_RM_Rsu_C.
    [Graphical view ]
    Pfami PF12008. EcoR124_C. 1 hit.
    PF04313. HSDR_N. 1 hit.
    PF04851. ResIII. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00348. hsdR. 1 hit.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
      Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
      Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15305 / DSM 20229.

    Entry informationi

    Entry nameiHSDR_STAS1
    AccessioniPrimary (citable) accession number: Q4A127
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3