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Q4A127 (HSDR_STAS1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-1 restriction enzyme R protein

EC=3.1.21.3
Alternative name(s):
Type I restriction enzyme R protein
Gene names
Name:hsdR
Ordered Locus Names:SSP0054
OrganismStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]
Taxonomic identifier342451 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length930 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Subunit R is required for both nuclease and ATPase activities, but not for modification By similarity.

Catalytic activity

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

Subunit structure

The type I restriction/modification system is composed of three polypeptides R, M and S By similarity.

Miscellaneous

Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases By similarity.

Sequence similarities

Belongs to the HsdR family.

Contains 1 helicase ATP-binding domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Type I site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 930930Type-1 restriction enzyme R protein
PRO_0000077272

Regions

Domain254 – 418165Helicase ATP-binding
Nucleotide binding268 – 2747ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4A127 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 559FA69A2AC96E24

FASTA930108,873
        10         20         30         40         50         60 
MVYQSEFALE TEMMEQLKSN GYETVTIRNE QQLLDNFRSI LNERHADKLN GDPLTDKEFQ 

        70         80         90        100        110        120 
RLLTMINGKG IFESARILRD KMPLKRDDES EVYLSFLDTR HWCQNKFQIT NQVSVDDTYK 

       130        140        150        160        170        180 
ARYDVTILIN GLPLVQIELK RRGIDINEAF NQVMRYRKQN YTGLFRYIQL FIISNGIDTR 

       190        200        210        220        230        240 
YISNNDGEIY KSHMFYWSDK ENNRINTLNE FTETFLRPCH IAKMISRYMI LNETDNILMA 

       250        260        270        280        290        300 
MRPYQVHAVE ALIHQATETS NNGYIWHTTG SGKTLTSFKA SQVLSEQDDI KKVIFLVDRK 

       310        320        330        340        350        360 
DLDSQTEEEF NKFSKGSVDK TNNTAQLVKQ LKDKSLPLIV TTIQKMSKAI QNNAEALDQY 

       370        380        390        400        410        420 
KTDKVVFIID ECHRSQFGDM HRIVRQHFNN AQYFGFTGTP RFEENQSQDG RSTADIFGRC 

       430        440        450        460        470        480 
LHTYLIKDAI HDGNVLGFSV DYINTIKAQN IDTETDELVE GINTDEVWLS DQRVELIARH 

       490        500        510        520        530        540 
ITENHDKYTR NRQYSAIFTV QSIPALVKYY DAFKKISKDY EHPLKVAGVF SYAANEERNE 

       550        560        570        580        590        600 
GEVDEAHSRG KLEEVIQDYN LNFGTNFSTD TFQEYFNHIS KNVKKGVKDN KIDVLIVVNM 

       610        620        630        640        650        660 
FLTGFDSKVL NTLYVDKNLK YHDLIQAYSR TNRVEKETKP FGKIVNYRDL KQNTDNALKL 

       670        680        690        700        710        720 
FSQTEDTDRV LMRDYDEYKA EFVDALAELK AVALKPQDMD QVQDENEKKA FVEAFRLVSK 

       730        740        750        760        770        780 
LVLRLKAFDE FDFTKANIGM NEQEFEDYKS KYFAIYDEVK PKRGEVEKVS ILNDIDFEIE 

       790        800        810        820        830        840 
ILRNDRINVS YIMDLVRQID LKDKAEQQRN RDQIRRMLDN ADDPTLRLKR DLLREFIDDV 

       850        860        870        880        890        900 
IPELSEEDNI DEAYILFENA KRESEFNQFA QQQAVDEQVL KDITGEYEYS GIVNQDHLKE 

       910        920        930 
LVGDKKLREK RQTKKAVTSF VEEVSEKYSS 

« Hide

References

[1]"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008934 Genomic DNA. Translation: BAE17199.1.
RefSeqYP_300144.1. NC_007350.1.

3D structure databases

ProteinModelPortalQ4A127.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING342451.SSP0054.

Protein family/group databases

REBASE11264. SsaAORF53P.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE17199; BAE17199; SSP0054.
GeneID3617259.
KEGGssp:SSP0054.
PATRIC19621587. VBIStaSap90642_0054.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0610.
HOGENOMHOG000003518.
KOK01153.
OMANNGYIWH.
OrthoDBEOG6JTCB4.

Enzyme and pathway databases

BioCycSSAP342451:GKFA-56-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR007409. Restrct_endonuc_type1_HsdR_N.
IPR004473. Restrct_endonuc_typeI_HsdR.
IPR022625. TypeI_RM_Rsu_C.
[Graphical view]
PfamPF12008. EcoR124_C. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00348. hsdR. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHSDR_STAS1
AccessionPrimary (citable) accession number: Q4A127
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families