Reviewed, UniProtKB/Swiss-Prot Q4A0Q1 (BETA_STAS1)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	SSP0196

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Choline dehydrogenase HAMAP MF_00750		PRO_0000205604
Regions
	Nucleotide binding	6 – 35	30	FAD Probable
Sites
	Active site	472	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4A0Q1-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 1CC3118F7D34A097	FASTA	560	62,402
	10 20 30 40 50 60 MKQSYDYIII GGGSAGSVLG SRISEDVSNN VLVLEAGRSD YPWDLLIQMP AALMYPAGNK 70 80 90 100 110 120 LYDWIYETTP EPHMDGRKVG HARGKVLGGS SSINGMIYQR GNPMDYEKWA KPEGMDSWDY 130 140 150 160 170 180 AHCLPYFKRL EKTFGATKDD QFRGHHGPIK LRRGPADNPL FQAFFDAGVE AGYNKTPDVN 190 200 210 220 230 240 GFRQEGFGPF DSQVHNGRRV SASRAYLHPA MKRKNLEVQT RAFVTKLNFE GNKVTGVTFK 250 260 270 280 290 300 KNGREHTVNA KEVILSGGAI NSPQLLQLSG IGDSEHLRSL GIEPRIHLPG VGENFEDHLE 310 320 330 340 350 360 VYVQHACKEP VSMQPSLNKL KMPFIGLQWI FGRKGAAASN HFEGGGFVRS NEDVDYPNLM 370 380 390 400 410 420 FHFLPIAVRY DGTKAPAAHG YQVHVGPMYS NSRGHLKIKS KDPFVKPDFV FNYLSTEEDK 430 440 450 460 470 480 REWVEAIKVA RNILGQKALD PYNGGEISPG PEVQTDEEII EWVKRDGETA LHPSCSCRMG 490 500 510 520 530 540 PASDEMSVVD PETFKVHGME NLRVVDASVM PRTTNGNIHS PVLMMAEKAA DIIRGKKPLD 550 560 PEYIDFYRHG VHDKDAGTIK

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE17341.1.
RefSeq	YP_300286.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3615110.
GenomeReviews	Gene locus SSP0196 in contig AP008934_GR.
KEGG	ssp:SSP0196.
NMPDR	fig|342451.4.peg.534.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4A0Q1.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP0196-MON.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_STAS1

Accession

Primary (citable) accession number: Q4A0Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)