Reviewed, UniProtKB/Swiss-Prot Q4A0E7 (ROCA_STAS1)

Names and origin

Protein names

Recommended name:
    1-pyrroline-5-carboxylate dehydrogenase
      Short name=P5C dehydrogenase
    EC=1.5.1.12

Gene names

Name:	rocA
Ordered Locus Names:	SSP0311

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)(+) + 2 H(2)O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	1-pyrroline-5-carboxylate dehydrogenase HAMAP MF_00733		PRO_0000056523
Sites
	Active site	286	1	By similarity
	Active site	320	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4A0E7-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 095E5E6121CEA548	FASTA	514	56,749
	10 20 30 40 50 60 MVVNYHNEPS IDFTDSKNVE SFKEALKKVK GELNQKIPLV INGEEKFTKD TYQSINPANT 70 80 90 100 110 120 TEVIAEVSKA TQKDVDDAFE AANEAYKSWK RWSHKDRAEF LIRVAAIIRR RKEEISAVMV 130 140 150 160 170 180 YEAGKPWDEA VGDAAEGIDF IEYYARSMME LADGKPVLDR EGEHNKYFYK PIGTGVTIPP 190 200 210 220 230 240 WNFPFAIMAG TTLAPVVAGN TVLLKPAEDT PLTAYKLMEI LEEAGLPKGV VNFVPGDPKE 250 260 270 280 290 300 IGDYLVDSVH THFVTFTGSR ATGTRIFERA AKVQDGQQFL KRVIAEMGGK DAIVVDKDID 310 320 330 340 350 360 TDLAAESIVS SAFGFSGQKC SACSRAIVHK DVYDEVLEKA VALTKNLTVG NTENNTYMGP 370 380 390 400 410 420 VINQKQFDKI KNYIEIGSKE GKLKQGGGTD DATGYFVEPT IIANLKSSDQ IMQEEIFGPV 430 440 450 460 470 480 VGFVKGKDFE ELLEIANDTD YGLTGAVITN NRENWIEAVE SYDVGNLYLN RGCTSAVVGY 490 500 510 HPFGGFKMSG TDAKTGSPDY LLNFLEQKVV SEMF

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE17456.1.
RefSeq	YP_300401.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3615883.
GenomeReviews	Gene locus SSP0311 in contig AP008934_GR.
KEGG	ssp:SSP0311.
NMPDR	fig|342451.4.peg.108.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4A0E7.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP0311-MON.
Family and domain databases
HAMAP	MF_00733. [Tree]
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR005932. d-1-pyrroline-5-COlate_DHase-2. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROCA_STAS1

Accession

Primary (citable) accession number: Q4A0E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)