ID PCP_STAS1 Reviewed; 213 AA. AC Q4A002; DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot. DT 13-SEP-2005, sequence version 1. DT 27-MAR-2024, entry version 108. DE RecName: Full=Pyrrolidone-carboxylate peptidase {ECO:0000255|HAMAP-Rule:MF_00417}; DE EC=3.4.19.3 {ECO:0000255|HAMAP-Rule:MF_00417}; DE AltName: Full=5-oxoprolyl-peptidase {ECO:0000255|HAMAP-Rule:MF_00417}; DE AltName: Full=Pyroglutamyl-peptidase I {ECO:0000255|HAMAP-Rule:MF_00417}; DE Short=PGP-I {ECO:0000255|HAMAP-Rule:MF_00417}; DE Short=Pyrase {ECO:0000255|HAMAP-Rule:MF_00417}; GN Name=pcp {ECO:0000255|HAMAP-Rule:MF_00417}; OrderedLocusNames=SSP0471; OS Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM OS 20229 / NCIMB 8711 / NCTC 7292 / S-41). OC Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae; OC Staphylococcus. OX NCBI_TaxID=342451; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41; RX PubMed=16135568; DOI=10.1073/pnas.0502950102; RA Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., RA Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.; RT "Whole genome sequence of Staphylococcus saprophyticus reveals the RT pathogenesis of uncomplicated urinary tract infection."; RL Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005). CC -!- FUNCTION: Removes 5-oxoproline from various penultimate amino acid CC residues except L-proline. {ECO:0000255|HAMAP-Rule:MF_00417}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Release of an N-terminal pyroglutamyl group from a CC polypeptide, the second amino acid generally not being Pro.; CC EC=3.4.19.3; Evidence={ECO:0000255|HAMAP-Rule:MF_00417}; CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00417}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00417}. CC -!- SIMILARITY: Belongs to the peptidase C15 family. {ECO:0000255|HAMAP- CC Rule:MF_00417}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AP008934; BAE17616.1; -; Genomic_DNA. DR RefSeq; WP_002482427.1; NZ_MTGA01000036.1. DR AlphaFoldDB; Q4A002; -. DR SMR; Q4A002; -. DR MEROPS; C15.001; -. DR KEGG; ssp:SSP0471; -. DR PATRIC; fig|342451.11.peg.476; -. DR eggNOG; COG2039; Bacteria. DR HOGENOM; CLU_043960_4_0_9; -. DR OrthoDB; 9779738at2; -. DR Proteomes; UP000006371; Chromosome. DR GO; GO:0005829; C:cytosol; IEA:InterPro. DR GO; GO:0016920; F:pyroglutamyl-peptidase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW. DR CDD; cd00501; Peptidase_C15; 1. DR Gene3D; 3.40.630.20; Peptidase C15, pyroglutamyl peptidase I-like; 1. DR HAMAP; MF_00417; Pyrrolid_peptidase; 1. DR InterPro; IPR000816; Peptidase_C15. DR InterPro; IPR016125; Peptidase_C15-like. DR InterPro; IPR036440; Peptidase_C15-like_sf. DR InterPro; IPR029762; PGP-I_bact-type. DR InterPro; IPR033694; PGPEP1_Cys_AS. DR InterPro; IPR033693; PGPEP1_Glu_AS. DR NCBIfam; TIGR00504; pyro_pdase; 1. DR PANTHER; PTHR23402; PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED; 1. DR PANTHER; PTHR23402:SF1; RE07960P; 1. DR Pfam; PF01470; Peptidase_C15; 1. DR PIRSF; PIRSF015592; Prld-crbxl_pptds; 1. DR PRINTS; PR00706; PYROGLUPTASE. DR SUPFAM; SSF53182; Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase); 1. DR PROSITE; PS01334; PYRASE_CYS; 1. DR PROSITE; PS01333; PYRASE_GLU; 1. PE 3: Inferred from homology; KW Cytoplasm; Hydrolase; Protease; Reference proteome; Thiol protease. FT CHAIN 1..213 FT /note="Pyrrolidone-carboxylate peptidase" FT /id="PRO_1000050142" FT ACT_SITE 78 FT /evidence="ECO:0000255|HAMAP-Rule:MF_00417" FT ACT_SITE 141 FT /evidence="ECO:0000255|HAMAP-Rule:MF_00417" FT ACT_SITE 165 FT /evidence="ECO:0000255|HAMAP-Rule:MF_00417" SQ SEQUENCE 213 AA; 23472 MW; 98C183CCC68A94E2 CRC64; MRILITGFDP FGGERVNPAL EAVKLLPDEI GAHKIDKLEI PTVFHKSKDV ILSQMKRYEY DIVLAIGQAG GRYELTPERV GINVDDARIA DNEGNQPIDE VIQVDGNAAY FSNLPVKRIT EAIKAQGIPS RLSNTAGTFV CNHILYQLGY LQATAFPKIK FGFIHVPFVP EQVTDKPEKP SMSLETIRIG LYAALEAIVE SGEDIKVALG ETH //