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Q49ZH2

- TOP3_STAS1

UniProt

Q49ZH2 - TOP3_STAS1

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Protein
DNA topoisomerase 3
Gene
topB, SSP0659
Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium; catalytic By similarity
Sitei60 – 601Interaction with DNA By similarity
Metal bindingi104 – 1041Magnesium; catalytic By similarity
Sitei167 – 1671Interaction with DNA By similarity
Sitei175 – 1751Interaction with DNA By similarity
Active sitei305 – 3051O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei307 – 3071Interaction with DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSSAP342451:GKFA-661-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III
Gene namesi
Name:topB
Ordered Locus Names:SSP0659
OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Taxonomic identifieri342451 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000006371: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 712712DNA topoisomerase 3UniRule annotation
PRO_0000286379Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi342451.SSP0659.

Structurei

3D structure databases

ProteinModelPortaliQ49ZH2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 135134Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1916Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000086847.
KOiK03169.
OMAiMTKHEER.
OrthoDBiEOG60CWM6.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q49ZH2-1 [UniParc]FASTAAdd to Basket

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MKSLIIAEKP SVGRDIANTL NINEKRNGYF ENNKYIVTWA LGHLVTNATP    50
EQYDASYKEW KLNDLPIIPN KMKTIVISKT RKQFSTVQSL INNNKVKDII 100
IATDAGREGE LVARLILDKA HNKKPTKRLW ISSVTNKAIK EGFNKLQDGR 150
KFNNLYHAAL ARSEADWIVG INATRALTTK YDAQLSLGRV QTPTIQLVQM 200
RQNEINHFKP QTYYTMKLNA AGLTFHCTKP QSHPDKTVLE SIKKAIDGQS 250
GHIASISKTH KKAYPQQLYN LTDLQQDAYK RFHLGPKETL NTLQTLYERH 300
KLVTYPRTDS NYLTDDMVDT LKDRLKAIMA TSLKDMAKAQ MSQTFSSKQR 350
FVNNNKVSDH HAIIPTEVRP DINQLSQRES KIYMMIAQRY LENLMPPHEY 400
EAIAIELKVG QHTFTFKDKV TTKLGFKAIY ENKESINTQI DQLQKGTKLN 450
VTKILIEEHE TTAPPYFNEG SLLKAMESPQ KFFDLSDKKH DKTLKDTGGI 500
GTVATRADII EKLFNMNAIE ARDGKIKVTS KGKQILELAP QKLTSPLLTA 550
EWEEKLLLIE QGKYNASQFI SEMKAFTNQV VNEIKESEQN YKHDNLTTTE 600
CPTCGKFMIK VKTKNGQMLV CQDPQCKTKK NVQRKTNARC PNCHKKMTLF 650
GRGKDAVYRC VCGHTETQAQ MDKRHKNKKS DKVNKKDLKK YMNNDEGIEN 700
NPFQDALKGL KF 712
Length:712
Mass (Da):81,398
Last modified:September 13, 2005 - v1
Checksum:iECC25B17796FAF11
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008934 Genomic DNA. Translation: BAE17804.1.
RefSeqiYP_300749.1. NC_007350.1.

Genome annotation databases

EnsemblBacteriaiBAE17804; BAE17804; SSP0659.
GeneIDi3615958.
KEGGissp:SSP0659.
PATRICi19622802. VBIStaSap90642_0661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008934 Genomic DNA. Translation: BAE17804.1 .
RefSeqi YP_300749.1. NC_007350.1.

3D structure databases

ProteinModelPortali Q49ZH2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 342451.SSP0659.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAE17804 ; BAE17804 ; SSP0659 .
GeneIDi 3615958.
KEGGi ssp:SSP0659.
PATRICi 19622802. VBIStaSap90642_0661.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000086847.
KOi K03169.
OMAi MTKHEER.
OrthoDBi EOG60CWM6.

Enzyme and pathway databases

BioCyci SSAP342451:GKFA-661-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00953. Topoisom_3_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01056. topB. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
    Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
    Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15305 / DSM 20229.

Entry informationi

Entry nameiTOP3_STAS1
AccessioniPrimary (citable) accession number: Q49ZH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: September 13, 2005
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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