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Q49ZH2 (TOP3_STAS1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 3

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase III
Gene names
Name:topB
Ordered Locus Names:SSP0659
OrganismStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]
Taxonomic identifier342451 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length712 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00953

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00953

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00953

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 712712DNA topoisomerase 3 HAMAP-Rule MF_00953
PRO_0000286379

Regions

Domain2 – 135134Toprim
Region186 – 1916Interaction with DNA By similarity

Sites

Active site3051O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding81Magnesium; catalytic By similarity
Metal binding1041Magnesium; catalytic By similarity
Site601Interaction with DNA By similarity
Site1671Interaction with DNA By similarity
Site1751Interaction with DNA By similarity
Site3071Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q49ZH2 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: ECC25B17796FAF11

FASTA71281,398
        10         20         30         40         50         60 
MKSLIIAEKP SVGRDIANTL NINEKRNGYF ENNKYIVTWA LGHLVTNATP EQYDASYKEW 

        70         80         90        100        110        120 
KLNDLPIIPN KMKTIVISKT RKQFSTVQSL INNNKVKDII IATDAGREGE LVARLILDKA 

       130        140        150        160        170        180 
HNKKPTKRLW ISSVTNKAIK EGFNKLQDGR KFNNLYHAAL ARSEADWIVG INATRALTTK 

       190        200        210        220        230        240 
YDAQLSLGRV QTPTIQLVQM RQNEINHFKP QTYYTMKLNA AGLTFHCTKP QSHPDKTVLE 

       250        260        270        280        290        300 
SIKKAIDGQS GHIASISKTH KKAYPQQLYN LTDLQQDAYK RFHLGPKETL NTLQTLYERH 

       310        320        330        340        350        360 
KLVTYPRTDS NYLTDDMVDT LKDRLKAIMA TSLKDMAKAQ MSQTFSSKQR FVNNNKVSDH 

       370        380        390        400        410        420 
HAIIPTEVRP DINQLSQRES KIYMMIAQRY LENLMPPHEY EAIAIELKVG QHTFTFKDKV 

       430        440        450        460        470        480 
TTKLGFKAIY ENKESINTQI DQLQKGTKLN VTKILIEEHE TTAPPYFNEG SLLKAMESPQ 

       490        500        510        520        530        540 
KFFDLSDKKH DKTLKDTGGI GTVATRADII EKLFNMNAIE ARDGKIKVTS KGKQILELAP 

       550        560        570        580        590        600 
QKLTSPLLTA EWEEKLLLIE QGKYNASQFI SEMKAFTNQV VNEIKESEQN YKHDNLTTTE 

       610        620        630        640        650        660 
CPTCGKFMIK VKTKNGQMLV CQDPQCKTKK NVQRKTNARC PNCHKKMTLF GRGKDAVYRC 

       670        680        690        700        710 
VCGHTETQAQ MDKRHKNKKS DKVNKKDLKK YMNNDEGIEN NPFQDALKGL KF 

« Hide

References

[1]"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008934 Genomic DNA. Translation: BAE17804.1.
RefSeqYP_300749.1. NC_007350.1.

3D structure databases

ProteinModelPortalQ49ZH2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING342451.SSP0659.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE17804; BAE17804; SSP0659.
GeneID3615958.
KEGGssp:SSP0659.
PATRIC19622802. VBIStaSap90642_0661.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000086847.
KOK03169.
OMATKHDEYF.
OrthoDBEOG60CWM6.
ProtClustDBPRK07726.

Enzyme and pathway databases

BioCycSSAP342451:GKFA-661-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00953. Topoisom_3_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01056. topB. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP3_STAS1
AccessionPrimary (citable) accession number: Q49ZH2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families