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Q49ZA3 (PTMCB_STAS1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
PTS system mannitol-specific EIICB component
Alternative name(s):
EIICB-Mtl
Short name=EII-Mtl

Including the following 2 domains:

  1. Mannitol permease IIC component
    Alternative name(s):
    PTS system mannitol-specific EIIC component
  2. Mannitol-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    PTS system mannitol-specific EIIB component
Gene names
Name:mtlA
Ordered Locus Names:SSP0728
OrganismStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]
Taxonomic identifier342451 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subunit structure

Homodimer By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similarities

Contains 1 PTS EIIB type-2 domain.

Contains 1 PTS EIIC type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 508508PTS system mannitol-specific EIICB component
PRO_0000186628

Regions

Transmembrane25 – 4521Helical; Potential
Transmembrane55 – 7521Helical; Potential
Transmembrane86 – 10621Helical; Potential
Transmembrane145 – 16521Helical; Potential
Transmembrane168 – 18821Helical; Potential
Transmembrane253 – 27321Helical; Potential
Transmembrane275 – 29521Helical; Potential
Transmembrane321 – 34121Helical; Potential
Domain19 – 351333PTS EIIC type-2
Domain420 – 50889PTS EIIB type-2

Sites

Active site4261Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q49ZA3 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 33926C8EA2CE175D

FASTA50854,700
        10         20         30         40         50         60 
MSQTETQENK GLGRKVQAFG SFLSSMIMPN IGAFIAWGFI AAIFIDGGWW PNKDLSELAG 

        70         80         90        100        110        120 
PMISYLIPLL IAYSGGRLIH EMRGGIIAAV ATMGVIVALP DTPMLLGAMI MGPLVGWLMK 

       130        140        150        160        170        180 
KTDEFIQPRT PQGFEMLFNN FSAGILGFIM TIVGFKILAP IMEFIMHILS LAVEALVHAH 

       190        200        210        220        230        240 
LLPLVSIIVE PAKIVFLNNA INHGVFTPLG ADQAASAGQS ILYTIESNPG PGLGILVAYM 

       250        260        270        280        290        300 
IFGKGTAKAT SYGAGIIHFL GGIHEIYFPY VLMRPLLFIA VILGGMTGVA TYSLLDFGFK 

       310        320        330        340        350        360 
SPASPGSFIV YMLNAPKGEF LHMVLGVLLA AIVSFIVAAL ILKFTKEPEE DLEAATEKME 

       370        380        390        400        410        420 
ASKGKKSSVS SKLKGNEDNN ATSTTASTST SENNEEQSEE ALLDNYDTEN VDAHDYSKVN 

       430        440        450        460        470        480 
HVIFACDAGM GSSAMGASML RNKFKKAGIQ DVNVTNTAIN QLPSDAQLVI TQKKLTDRAI 

       490        500 
KQVPNAIHIS VDNFLNSPRY DELLENLK

« Hide

References

[1]"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP008934 Genomic DNA. Translation: BAE17873.1.
RefSeqYP_300818.1. NC_007350.1.

3D structure databases

ProteinModelPortalQ49ZA3.
SMRQ49ZA3. Positions 417-508.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ49ZA3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000048042; EBSTAP00000046411; EBSTAG00000048039.
GeneID3615929.
GenomeReviewsGene locus SSP0728 in contig AP008934_GR.
KEGGssp:SSP0728.
NMPDRfig|342451.4.peg.1141.
PATRIC19622958. VBIStaSap90642_0730.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2213.
GeneTreeEBGT00050000024561.
HOGENOMHBG296680.
OMAKSARTAN.
PhylomeDBQ49ZA3.
ProtClustDBCLSK2460866.

Enzyme and pathway databases

BioCycSSAP342451:SSP0728-MONOMER.

Family and domain databases

InterProIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004718. PTS_IIC_mtl.
[Graphical view]
KOK02799.
K02800.
PfamPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00851. MtlA. 1 hit.
PROSITEPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTMCB_STAS1
AccessionPrimary (citable) accession number: Q49ZA3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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