Reviewed, UniProtKB/Swiss-Prot Q49ZA3 (PTMCB_STAS1)

Names and origin

Protein names

Recommended name:
    PTS system mannitol-specific EIICB component
Alternative name(s):
    EIICB-Mtl
      Short name=EII-Mtl
Including the following 2 domains:
    1- Recommended name:
            Mannitol permease IIC component
        Alternative name(s):
            PTS system mannitol-specific EIIC component
    2- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system mannitol-specific EIIB component

Gene names

Name:	mtlA
Ordered Locus Names:	SSP0728

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	PTS system mannitol-specific EIICB component		PRO_0000186628
Regions
	Transmembrane	25 – 45	21	Potential
	Transmembrane	55 – 75	21	Potential
	Transmembrane	86 – 106	21	Potential
	Transmembrane	145 – 165	21	Potential
	Transmembrane	168 – 188	21	Potential
	Transmembrane	253 – 273	21	Potential
	Transmembrane	275 – 295	21	Potential
	Transmembrane	321 – 341	21	Potential
	Domain	19 – 351	333	PTS EIIC type-2
	Domain	420 – 508	89	PTS EIIB type-2
Sites
	Active site	426	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49ZA3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 33926C8EA2CE175D	FASTA	508	54,700
	10 20 30 40 50 60 MSQTETQENK GLGRKVQAFG SFLSSMIMPN IGAFIAWGFI AAIFIDGGWW PNKDLSELAG 70 80 90 100 110 120 PMISYLIPLL IAYSGGRLIH EMRGGIIAAV ATMGVIVALP DTPMLLGAMI MGPLVGWLMK 130 140 150 160 170 180 KTDEFIQPRT PQGFEMLFNN FSAGILGFIM TIVGFKILAP IMEFIMHILS LAVEALVHAH 190 200 210 220 230 240 LLPLVSIIVE PAKIVFLNNA INHGVFTPLG ADQAASAGQS ILYTIESNPG PGLGILVAYM 250 260 270 280 290 300 IFGKGTAKAT SYGAGIIHFL GGIHEIYFPY VLMRPLLFIA VILGGMTGVA TYSLLDFGFK 310 320 330 340 350 360 SPASPGSFIV YMLNAPKGEF LHMVLGVLLA AIVSFIVAAL ILKFTKEPEE DLEAATEKME 370 380 390 400 410 420 ASKGKKSSVS SKLKGNEDNN ATSTTASTST SENNEEQSEE ALLDNYDTEN VDAHDYSKVN 430 440 450 460 470 480 HVIFACDAGM GSSAMGASML RNKFKKAGIQ DVNVTNTAIN QLPSDAQLVI TQKKLTDRAI 490 500 KQVPNAIHIS VDNFLNSPRY DELLENLK

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE17873.1.
RefSeq	YP_300818.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3615929.
GenomeReviews	Gene locus SSP0728 in contig AP008934_GR.
KEGG	ssp:SSP0728.
NMPDR	fig|342451.4.peg.1141.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q49ZA3.
OMA	Q49ZA3. KSARTAN.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP0728-MON.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR003501. PTS_IIB_lac. IPR004718. PTS_IIC_mtl. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00851. mtlA. 1 hit.
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTMCB_STAS1

Accession

Primary (citable) accession number: Q49ZA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)