Reviewed, UniProtKB/Swiss-Prot Q49Z71 (MURA1_STAS1)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 1
    UDP-N-acetylglucosamine enolpyruvyl transferase 1
      Short name=EPT 1

Gene names

Name:	murA1
Ordered Locus Names:	SSP0760

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable HAMAP MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 HAMAP MF_00111		PRO_0000231269
Sites
	Active site	118	1	Proton donor By similarity
	Binding site	118	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49Z71-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: FE032B0A1C3B85C8	FASTA	419	44,527
	10 20 30 40 50 60 MAQEVIKIRG GQTLKGEVNN HGAKNSAVAI IPAAILAEDK VTIEGLPEIS DVETLVSLLG 70 80 90 100 110 120 DLNIKTELDG MTLNVDPNEI ENAPLPNNKV ESLRASYYMM GAMLGRFKKC VIGLPGGCPL 130 140 150 160 170 180 GPRPIDQHIK GFKALGAKID ESSSTSMKIE ADKLTGANIF LDIVSVGATI NIMLAAVRAE 190 200 210 220 230 240 GQTVIENAAK EPEVVDVANF LTSLGADIKG AGTSTIKING VEHLHGSHHQ VIPDRIEAGT 250 260 270 280 290 300 YMCIAAACGE DITINNIIPT HVEPLTVKLK ELGVDIQVGD DSAVIQRSTP YASVDIKTLV 310 320 330 340 350 360 YPGFATDLQQ PITPLLFMAD GVSFVTETIY PARFRHVDEL KNMGADISAD NGTATIKPSK 370 380 390 400 410 LTGAEVYASD LRAGACLIVA GLLAEGVTTI YNVKHIYRGY TNIVSTLKSL GADIWTEEV

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008934 Genomic DNA. Translation: BAE17905.1.
RefSeq	YP_300850.1.
3D structure databases
SMR	Q49Z71. Positions 6-417.
ModBase	Search...
Protein-protein interaction databases
STRING	Q49Z71.
Genome annotation databases
GeneID	3615825.
GenomeReviews	Gene locus SSP0760 in contig AP008934_GR.
KEGG	ssp:SSP0760.
NMPDR	fig|342451.4.peg.1275.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0766.
HOGENOM	HBG482701.
OMA	LESFICK.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP0760-MONOMER.
Family and domain databases
HAMAP	MF_00111. MurA. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA1_STAS1

Accession

Primary (citable) accession number: Q49Z71

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)