Reviewed, UniProtKB/Swiss-Prot Q49Z47 (MURA2_STAS1)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 2
    UDP-N-acetylglucosamine enolpyruvyl transferase 2
      Short name=EPT 2

Gene names

Name:	murA2
Ordered Locus Names:	SSP0784

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity.
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000231270
Sites
	Active site	119	1	Proton donor By similarity
	Binding site	119	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49Z47-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 74C0552CFE6BF168	FASTA	421	45,031
	10 20 30 40 50 60 MDMIEINGGN RLTGEVKVSG AKNAVLPVLT ASLLASEGVS KLMNVPALSD VETINNVIST 70 80 90 100 110 120 LNAEVSYDKD EESVTVDATK ELNEEAPYEY VSKMRASILV MGPLLARLGH AKVALPGGCA 130 140 150 160 170 180 IGSRPIEQHI KGFEELGADI HMDGGFIYAS TENGLKGTTI HLDFPSVGAT QNIIMAASLA 190 200 210 220 230 240 EGKTVLENVA REPEIVDLAN YINEMGGNVS GAGTDTIIIH GVETLHGVEH AIIPDRIEAG 250 260 270 280 290 300 TLLIAGAITR GDVLVNGAIK EHMTSLVYKL EEMGVNLDYQ DDAIRVRVED ELKPVDIKTL 310 320 330 340 350 360 PHPGFPTDMQ SQMMALLLTA EGHKVVTETV FENRFMHVAE FKRMNAKISV EGRSAKIEGK 370 380 390 400 410 420 SELQGAQVKA TDLRAAAALI LAGLVADGTT QVTELKHLDR GYVDLHGKLE ALGADIKRTQ A

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE17929.1.
RefSeq	YP_300874.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3615751.
GenomeReviews	Gene locus SSP0784 in contig AP008934_GR.
KEGG	ssp:SSP0784.
NMPDR	fig|342451.4.peg.1369.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q49Z47.
OMA	Q49Z47. VSRVYHL.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP0784-MON.
Family and domain databases
HAMAP	MF_00111. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 1 hit.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_STAS1

Accession

Primary (citable) accession number: Q49Z47

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)