Q49Z16 (THD1_STAS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine dehydratase biosynthetic
EC=4.3.1.19

Alternative name(s):
Threonine deaminase

Gene names

Name:	ilvA
Ordered Locus Names:	SSP0817

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia By similarity.
Catalytic activity	L-threonine = 2-oxobutanoate + NH3.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.
Sequence similarities	Belongs to the serine/threonine dehydratase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Isoleucine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-threonine ammonia-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Threonine dehydratase biosynthetic		PRO_0000234315
Amino acid modifications
	Modified residue	56	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49Z16 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 4BADCC7143465109	FASTA	422	46,840
	10 20 30 40 50 60 MTVKTTVSSK DIDEAYLQLK DIVKETPLQK DHYLSQKYDC KVYLKREDLQ WVRSFKLRGA 70 80 90 100 110 120 YNAIIALDEA DRQNGITCAS AGNHAQGVAY TASKLNLNAV IFMPVTTPLQ KINQVKFFGG 130 140 150 160 170 180 DNTEVVLTGD TFDDCLKEAL VYTEENKMNF IDPFNNIYTI AGQGTLAKEI LEQSKDNDIQ 190 200 210 220 230 240 FDYLFAAIGG GGLISGVGTY FKTHSPETSI IGVEPAGAAS MYTSVVLENQ LVTLPDIDKF 250 260 270 280 290 300 VDGASVARVG QITFDISKDI VDDYIQVHEG AVCSTILDMY SKQAIIAEPA GALSIAALDQ 310 320 330 340 350 360 YQAEIKGKTV VCVVSGGNND INRMKEIEER SLLFEEMKHY FILNFPQRPG ALREFVNEVL 370 380 390 400 410 420 GPKDDITKFE YLKKSSQNTG TVIIGIQLNN HKDLGHLKAN VDEFDKSNIY INENKMLYSL LI

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008934 Genomic DNA. Translation: BAE17962.1.
RefSeq	YP_300907.1. NC_007350.1.
3D structure databases
ProteinModelPortal	Q49Z16.
ModBase	Search...
Protein-protein interaction databases
STRING	Q49Z16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000048162; EBSTAP00000046531; EBSTAG00000048159.
GeneID	3617339.
GenomeReviews	Gene locus SSP0817 in contig AP008934_GR.
KEGG	ssp:SSP0817.
NMPDR	fig|342451.4.peg.1233.
PATRIC	19623146. VBIStaSap90642_0819.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1171.
GeneTree	EBGT00050000024421.
HOGENOM	HBG714501.
OMA	IVCASAG.
PhylomeDB	Q49Z16.
ProtClustDB	PRK08639.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP0817-MONOMER.
Family and domain databases
InterPro	IPR001926. PyrdxlP-dep_enz_bsu. IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS. IPR001721. Thr_deHydtase_C. IPR011820. Threonine_deHydtase. [Graphical view]
KO	K01754.
Pfam	PF00291. PALP. 1 hit. PF00585. Thr_dehydrat_C. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR02079. THD1. 1 hit.
PROSITE	PS00165. DEHYDRATASE_SER_THR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THD1_STAS1

Accession

Primary (citable) accession number: Q49Z16

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program