Reviewed, UniProtKB/Swiss-Prot Q49Z08 (ILVD1_STAS1)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 1
      Short name=DAD 1
    EC=4.2.1.9

Gene names

Name:	ilvD1
Ordered Locus Names:	SSP0825

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Dihydroxy-acid dehydratase 1 HAMAP MF_00012		PRO_0000225426
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	194	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q49Z08-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: E8ABDDD159DF6DEF	FASTA	562	60,139
	10 20 30 40 50 60 MRSDMIKKGD QQAPARSLLH ATGALDKPTD MNKPFVAICN SYIDIVPGHV HLRELADIAK 70 80 90 100 110 120 EAIREAGAIP FEFDTIGVDD GIAMGHIGMR YSLPSREIIA DAAETVINAH WFDGVFYIPN 130 140 150 160 170 180 CDKITPGMLL ASVRTNVPAI FCSGGPMKAG LSSEGKALTL SSMFEAVGAF KDGSISKDEF 190 200 210 220 230 240 LDMEQNACPT CGSCSGMFTA NSMNCLMEVL GLALPYNGTA LAVSDQRREM IREAAFKLVD 250 260 270 280 290 300 NIKNDLKPKD IVTREAIDDA FALDMAMGGS TNTVLHTLAI ANEAGIDYDL TRINEIAKKT 310 320 330 340 350 360 PYLSKIAPSS SYSMHDVHEA GGVPAIINEL FKKEGTLHPD RITATGKTLR ENNEDKEILN 370 380 390 400 410 420 SDVIRHLDNP YDKQGGLSIL YGNIAPKGAV IKVGGVDPSI KVFKGKAICF DSHDEAVEAI 430 440 450 460 470 480 DNHDVREGHV VVIRYEGPKG GPGMPEMLAP TSSIVGRGLG KDVALITDGR FSGATRGIAV 490 500 510 520 530 540 GHISPEAAAG GPIGLINDGD EITIDLTNRT LDVNVSTEEL DARKQNVKPF KAKVKTGYLA 550 560 RYTALVTSAN TGGIMQVPEN LI

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE17970.1.
RefSeq	YP_300915.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q49Z08.
Genome annotation databases
GeneID	3617347.
GenomeReviews	Gene locus SSP0825 in contig AP008934_GR.
KEGG	ssp:SSP0825.
NMPDR	fig|342451.4.peg.1241.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q49Z08.
OMA	ERISHTV.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP0825-MON.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD1_STAS1

Accession

Primary (citable) accession number: Q49Z08

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)