Reviewed, UniProtKB/Swiss-Prot Q49YV3 (PUR8_STAS1)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate lyase
      Short name=ASL
    EC=4.3.2.2
Alternative name(s):
    Adenylosuccinase
      Short name=ASase

Gene names

Name:	purB
Ordered Locus Names:	SSP0882

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP. (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 5/5.
Sequence similarities	Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine ribonucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate AMP-lyase (fumarate-forming) activity Inferred from electronic annotation. Source: EC N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Adenylosuccinate lyase		PRO_0000259985
Sites
	Active site	68	1	Proton donor By similarity
	Active site	141	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49YV3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 0D78B0FDCDE23205	FASTA	431	49,412
	10 20 30 40 50 60 MIERYSREEM SNIWTDQNRY EAWLEVEILA CEAWSKLGDI PAEDVKKIRE NAKVDVARAQ 70 80 90 100 110 120 EIEQETRHDV VAFTRQVSET LGEERKWVHY GLTSTDVVDT ALSYVIKQAN EIIKKDLERF 130 140 150 160 170 180 IDVLAQKAKD YKYTLMMGRT HGVHAEPTTF GVKMALWYTE MKRNLERFKQ VRKEIEVGKM 190 200 210 220 230 240 SGAVGTFANI PPEIEAYVCE HLGLDAAPVS TQTLQRDRHA YYVATLALIS TSMEKFAVEI 250 260 270 280 290 300 RNLQKTETRE VEEAFAKGQK GSSAMPHKRN PIGSENITGI ARVIRGYVTT AYENVPLWHE 310 320 330 340 350 360 RDISHSSAER IMLPDVTIAL DYGLNRFTNI VERLTVFEDN MLANIDKTFG LIYSQRVLLA 370 380 390 400 410 420 LINKGLAREA AYDKVQPKAM ESWETKTPFR TLIEEDATIT DLLTKEDLDE CFNPKHHLNQ 430 VDTIFQRAGL E

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE18027.1.
RefSeq	YP_300972.1.
3D structure databases
SMR	Q49YV3. Positions 2-430.
ModBase	Search...
Genome annotation databases
GeneID	3617078.
GenomeReviews	Gene locus SSP0882 in contig AP008934_GR.
KEGG	ssp:SSP0882.
NMPDR	fig|342451.4.peg.652.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q49YV3.
OMA	Q49YV3. TIGRSHG.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP0882-MON.
Family and domain databases
InterPro	IPR019468. Adenylosuccinate_lyase_C. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR004769. Pur_lyase. [Graphical view]
Pfam	PF10397. ADSL_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. DCRYSTALLIN. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00928. purB. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR8_STAS1

Accession

Primary (citable) accession number: Q49YV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)