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Q49YV3 (PUR8_STAS1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenylosuccinate lyase
Short name=ASL
EC=4.3.2.2
Alternative name(s):
Adenylosuccinase
Short name=ASase
Gene names
Name:purB
Ordered Locus Names:SSP0882
OrganismStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]
Taxonomic identifier342451 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length431 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP.

(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.

Sequence similarities

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 431431Adenylosuccinate lyase
PRO_0000259985

Sites

Active site681Proton donor By similarity
Active site1411Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q49YV3 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 0D78B0FDCDE23205

FASTA43149,412
        10         20         30         40         50         60 
MIERYSREEM SNIWTDQNRY EAWLEVEILA CEAWSKLGDI PAEDVKKIRE NAKVDVARAQ 

        70         80         90        100        110        120 
EIEQETRHDV VAFTRQVSET LGEERKWVHY GLTSTDVVDT ALSYVIKQAN EIIKKDLERF 

       130        140        150        160        170        180 
IDVLAQKAKD YKYTLMMGRT HGVHAEPTTF GVKMALWYTE MKRNLERFKQ VRKEIEVGKM 

       190        200        210        220        230        240 
SGAVGTFANI PPEIEAYVCE HLGLDAAPVS TQTLQRDRHA YYVATLALIS TSMEKFAVEI 

       250        260        270        280        290        300 
RNLQKTETRE VEEAFAKGQK GSSAMPHKRN PIGSENITGI ARVIRGYVTT AYENVPLWHE 

       310        320        330        340        350        360 
RDISHSSAER IMLPDVTIAL DYGLNRFTNI VERLTVFEDN MLANIDKTFG LIYSQRVLLA 

       370        380        390        400        410        420 
LINKGLAREA AYDKVQPKAM ESWETKTPFR TLIEEDATIT DLLTKEDLDE CFNPKHHLNQ 

       430 
VDTIFQRAGL E

« Hide

References

[1]"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP008934 Genomic DNA. Translation: BAE18027.1.
RefSeqYP_300972.1. NC_007350.1.

3D structure databases

ProteinModelPortalQ49YV3.
SMRQ49YV3. Positions 2-430.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ49YV3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000047309; EBSTAP00000045678; EBSTAG00000047306.
GeneID3617078.
GenomeReviewsGene locus SSP0882 in contig AP008934_GR.
KEGGssp:SSP0882.
NMPDRfig|342451.4.peg.652.
PATRIC19623270. VBIStaSap90642_0881.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0015.
GeneTreeEBGT00050000024473.
HOGENOMHBG499008.
OMAHACDALA.
PhylomeDBQ49YV3.
ProtClustDBPRK08937.

Enzyme and pathway databases

BioCycSSAP342451:SSP0882-MONOMER.

Family and domain databases

InterProIPR019468. AdenyloSucc_lyase_C.
IPR003031. D_crystallin.
IPR000362. Fumarate_lyase.
IPR020557. Fumarate_lyase_CS.
IPR008948. L-Aspartase-like.
IPR024083. L-Aspartase-like_N.
IPR022761. Lyase1_N.
IPR004769. Pur_lyase.
[Graphical view]
Gene3DG3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KOK01756.
PANTHERPTHR11444:SF2. PTHR11444:SF2. 1 hit.
PfamPF10397. ADSL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00145. ARGSUCLYASE.
PR00149. FUMRATELYASE.
SMARTSM00998. ADSL_C. 1 hit.
[Graphical view]
SUPFAMSSF48557. L-Aspartase-like. 1 hit.
TIGRFAMsTIGR00928. PurB. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR8_STAS1
AccessionPrimary (citable) accession number: Q49YV3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: September 13, 2005
Last modified: January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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