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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSAP342451:GKFA-911-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Asp/Glu-ADT subunit BUniRule annotation
Gene namesi
Name:gatBUniRule annotation
Ordered Locus Names:SSP0892
OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Taxonomic identifieri342451 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000006371 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BPRO_0000241279Add
BLAST

Proteomic databases

PRIDEiQ49YU3.

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi342451.SSP0892.

Structurei

3D structure databases

ProteinModelPortaliQ49YU3.
SMRiQ49YU3. Positions 3-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0064.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
OrthoDBiEOG6RJV5B.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR023168. GatB_Yqey_C.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q49YU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFETVIGLE VHVELKTDSK MFSPAPAHFG AKPNSNTNVI DLAYPGVLPV
60 70 80 90 100
VNRRAVDWAM RASMALNMEI ATESKFDRKN YFYPDNPKAY QISQFDQPIG
110 120 130 140 150
ENGYIDIEVD GETKRIGITR LHMEEDAGKS THKDGYSLVD LNRQGTPLIE
160 170 180 190 200
IVSEPDIRSP QEAYAYLEKL RSIIQYTGVS DCKMEEGSLR CDANISLRPY
210 220 230 240 250
GQEEFGTKAE LKNLNSFTYV RKGLEYEEKR QEEELLNGGE ILQETRRFDE
260 270 280 290 300
SNGKTLLMRV KEGSDDYRYF PEPDIVPLYV DEEWKERVRQ TIPELPDERK
310 320 330 340 350
EKYVNQYGLP AYDAHVLTLT KEMSDFFEGA VAEGADVKLT SNWLMGGVNE
360 370 380 390 400
YLNKNQIDLL DTKLTPENLA GMIKLIEDGT MSSKIAKKVF PELAENGGDA
410 420 430 440 450
KQIMEDKGLV QISDEATLLK FVNEALDNNE QSIEDYKNGK GKAMGFLVGQ
460 470
IMKASKGQAN PQLVNQLLKQ ELDKR
Length:475
Mass (Da):53,829
Last modified:September 13, 2005 - v1
Checksum:i62202720AF90ED5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA. Translation: BAE18037.1.
RefSeqiWP_011302768.1. NC_007350.1.

Genome annotation databases

EnsemblBacteriaiBAE18037; BAE18037; SSP0892.
GeneIDi23780604.
KEGGissp:SSP0892.
PATRICi19623290. VBIStaSap90642_0891.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA. Translation: BAE18037.1.
RefSeqiWP_011302768.1. NC_007350.1.

3D structure databases

ProteinModelPortaliQ49YU3.
SMRiQ49YU3. Positions 3-406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi342451.SSP0892.

Proteomic databases

PRIDEiQ49YU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE18037; BAE18037; SSP0892.
GeneIDi23780604.
KEGGissp:SSP0892.
PATRICi19623290. VBIStaSap90642_0891.

Phylogenomic databases

eggNOGiCOG0064.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
OrthoDBiEOG6RJV5B.

Enzyme and pathway databases

BioCyciSSAP342451:GKFA-911-MONOMER.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR023168. GatB_Yqey_C.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
    Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
    Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15305 / DSM 20229.

Entry informationi

Entry nameiGATB_STAS1
AccessioniPrimary (citable) accession number: Q49YU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: September 13, 2005
Last modified: July 22, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.