Q49YU3 (GATB_STAS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	SSP0892

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus saprophyticus › Staphylococcus saprophyticus subsp. saprophyticus

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity.
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121		PRO_0000241279

Sequences

	Sequence		Length	Mass (Da)
	Q49YU3 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 62202720AF90ED5D	FASTA	475	53,829
	10 20 30 40 50 60 MHFETVIGLE VHVELKTDSK MFSPAPAHFG AKPNSNTNVI DLAYPGVLPV VNRRAVDWAM 70 80 90 100 110 120 RASMALNMEI ATESKFDRKN YFYPDNPKAY QISQFDQPIG ENGYIDIEVD GETKRIGITR 130 140 150 160 170 180 LHMEEDAGKS THKDGYSLVD LNRQGTPLIE IVSEPDIRSP QEAYAYLEKL RSIIQYTGVS 190 200 210 220 230 240 DCKMEEGSLR CDANISLRPY GQEEFGTKAE LKNLNSFTYV RKGLEYEEKR QEEELLNGGE 250 260 270 280 290 300 ILQETRRFDE SNGKTLLMRV KEGSDDYRYF PEPDIVPLYV DEEWKERVRQ TIPELPDERK 310 320 330 340 350 360 EKYVNQYGLP AYDAHVLTLT KEMSDFFEGA VAEGADVKLT SNWLMGGVNE YLNKNQIDLL 370 380 390 400 410 420 DTKLTPENLA GMIKLIEDGT MSSKIAKKVF PELAENGGDA KQIMEDKGLV QISDEATLLK 430 440 450 460 470 FVNEALDNNE QSIEDYKNGK GKAMGFLVGQ IMKASKGQAN PQLVNQLLKQ ELDKR

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008934 Genomic DNA. Translation: BAE18037.1.
RefSeq	YP_300982.1. NC_007350.1.
3D structure databases
ProteinModelPortal	Q49YU3.
SMR	Q49YU3. Positions 3-406.
ModBase	Search...
Protein-protein interaction databases
STRING	342451.SSP0892.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAE18037; BAE18037; SSP0892.
GeneID	3617088.
KEGG	ssp:SSP0892.
PATRIC	19623290. VBIStaSap90642_0891.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0064.
HOGENOM	HOG000223742.
KO	K02434.
OMA	KNYFYAD.
ProtClustDB	PRK05477.
Family and domain databases
Gene3D	1.10.10.410. 1 hit.
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR023168. GatB_Yqey_C. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_STAS1

Accession

Primary (citable) accession number: Q49YU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program