Reviewed, UniProtKB/Swiss-Prot Q49YP8 (FUMC_STAS1)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	SSP0944

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161320
Regions
	Region	127 – 130	4	B site By similarity
	Region	137 – 139	3	Substrate binding By similarity
Sites
	Binding site	99	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49YP8-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: BFFD5A5151E46EAE	FASTA	461	50,956
	10 20 30 40 50 60 MSVRIEHDTF GEIEVPADKY WGAQTERSKR NFPVGKERMP IEVVYGFAQL KRGAALANHA 70 80 90 100 110 120 LGKLSDAKKD AIVYACDRVL NKELDEHFPL VVWQTGSGTQ SNMNVNEVVS YVANTYLKEQ 130 140 150 160 170 180 GIDESIHPND DVNKSQSSND TFPTAMHVAL YNEVETKLEP ALKTLRDTFK QKEEQYHDII 190 200 210 220 230 240 KIGRTHLQDA TPIRLGQEIS GWRYMLDKCE TLLSESKAHI LNLAIGGTAV GTGINAHPEF 250 260 270 280 290 300 GDKVAKFIAE NTGYPFVSSE NKFHALTAHD EVVQLHGTLK ALATDLMKIA NDVRWLASGP 310 320 330 340 350 360 RAGLAEISIP ENEPGSSIMP GKVNPTQCEM LTMVAVQVMG NDTAVGIASS QGNFELNVYK 370 380 390 400 410 420 PVILLNTLQS IYLLADGMDT FNNNCAVGIE PIPENIDNYL NQSLMLVTAL NPHIGYEKAA 430 440 450 460 SIAKKAHREG LTLKESAIDS GYVTEEQFEQ WIKPEDMVEP K

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE18089.1.
RefSeq	YP_301034.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3616126.
GenomeReviews	Gene locus SSP0944 in contig AP008934_GR.
KEGG	ssp:SSP0944.
NMPDR	fig|342451.4.peg.937.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q49YP8.
OMA	Q49YP8. GSQGHFE.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP0944-MON.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. DCRYSTALLIN. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_STAS1

Accession

Primary (citable) accession number: Q49YP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)