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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSAP342451:GKFA-1065-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:SSP1006
OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Taxonomic identifieri342451 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000006371 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Leucine--tRNA ligasePRO_1000009441Add
BLAST

Proteomic databases

PRIDEiQ49YI8.

Interactioni

Protein-protein interaction databases

STRINGi342451.SSP1006.

Structurei

3D structure databases

ProteinModelPortaliQ49YI8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q49YI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYSHNEIEK KWQDYWEANK TFKTSDNLGQ KKFYALDMFP YPSGAGLHVG
60 70 80 90 100
HPEGYTATDI ISRYKRMLGY NVLHPMGWDA FGLPAEQYAL DTGNDPREFT
110 120 130 140 150
KENIQTFKRQ IKELGFSYDW DREVNTTDPE YYKWTQWIFI QLYNKGLAYV
160 170 180 190 200
DEVAVNWCPA LGTVLSNEEV IDGVSERGGH PVYRRPMKQW VLKITEYADR
210 220 230 240 250
LLEDLDELDW PESLKDMQRN WIGRSEGASV AFDVKDYNEQ IEVFTTRPDT
260 270 280 290 300
IYGASFLVLS PEHELVDLIT TEDNKDDVEA YQKEAAKKSD LERTGLSKEK
310 320 330 340 350
SGVFTGAYAV NPLSGQQVPI WIADYVLSTY GTGAVMAVPS GDQRDYEFAK
360 370 380 390 400
TFDLPIIEVI EGGDMSKEAY TGDGPHINSG ELNGLYNEAA IEKAIELLEN
410 420 430 440 450
KNAGTRKVNY KLRDWLFSRQ RYWGEPIPVI HWEDGSMTTV PEDELPLLLP
460 470 480 490 500
ETDEIKPSGT GESPLANIDE FVNVVDEATG MKGRRETNTM PQWAGSCWYY
510 520 530 540 550
LRYIDPDNEQ MLADPEKLKH WLPVDLYIGG VEHAVLHLLY ARFWHKVLFD
560 570 580 590 600
LGVVPTKEPF QKLFNQGMIL GEGNEKMSKS KGNVINPDDI VKSHGADTLR
610 620 630 640 650
LYEMFMGPLD AAIAWSENGL DGSRRFLDRI WRLLITEDGS ISNKVVNNHS
660 670 680 690 700
KALDKSYHQT VKKVTEDYNS LNFNTAISQL MVFINDCYKA EEIYKPYIEG
710 720 730 740 750
FIKMLAPIAP HISEELWSRL GHDETITYQP WPSYDEALLV DDEIEIVVQV
760 770 780 790 800
NGKVRAKINV SKDIAKEEME QIALDNEHVK SEIEGKDIKK VIAVPKKLVN

IVAK
Length:804
Mass (Da):91,860
Last modified:September 13, 2005 - v1
Checksum:i2337B733107FF07C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA. Translation: BAE18151.1.
RefSeqiWP_011302857.1. NC_007350.1.

Genome annotation databases

EnsemblBacteriaiBAE18151; BAE18151; SSP1006.
GeneIDi23780458.
KEGGissp:SSP1006.
PATRICi19623596. VBIStaSap90642_1005.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA. Translation: BAE18151.1.
RefSeqiWP_011302857.1. NC_007350.1.

3D structure databases

ProteinModelPortaliQ49YI8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi342451.SSP1006.

Proteomic databases

PRIDEiQ49YI8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE18151; BAE18151; SSP1006.
GeneIDi23780458.
KEGGissp:SSP1006.
PATRICi19623596. VBIStaSap90642_1005.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciSSAP342451:GKFA-1065-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
    Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
    Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15305 / DSM 20229.

Entry informationi

Entry nameiSYL_STAS1
AccessioniPrimary (citable) accession number: Q49YI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: July 22, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.