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Q49YI8 (SYL_STAS1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:SSP1006
OrganismStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]
Taxonomic identifier342451 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009441

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q49YI8 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 2337B733107FF07C

FASTA80491,860
        10         20         30         40         50         60 
MNYSHNEIEK KWQDYWEANK TFKTSDNLGQ KKFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
ISRYKRMLGY NVLHPMGWDA FGLPAEQYAL DTGNDPREFT KENIQTFKRQ IKELGFSYDW 

       130        140        150        160        170        180 
DREVNTTDPE YYKWTQWIFI QLYNKGLAYV DEVAVNWCPA LGTVLSNEEV IDGVSERGGH 

       190        200        210        220        230        240 
PVYRRPMKQW VLKITEYADR LLEDLDELDW PESLKDMQRN WIGRSEGASV AFDVKDYNEQ 

       250        260        270        280        290        300 
IEVFTTRPDT IYGASFLVLS PEHELVDLIT TEDNKDDVEA YQKEAAKKSD LERTGLSKEK 

       310        320        330        340        350        360 
SGVFTGAYAV NPLSGQQVPI WIADYVLSTY GTGAVMAVPS GDQRDYEFAK TFDLPIIEVI 

       370        380        390        400        410        420 
EGGDMSKEAY TGDGPHINSG ELNGLYNEAA IEKAIELLEN KNAGTRKVNY KLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPVI HWEDGSMTTV PEDELPLLLP ETDEIKPSGT GESPLANIDE FVNVVDEATG 

       490        500        510        520        530        540 
MKGRRETNTM PQWAGSCWYY LRYIDPDNEQ MLADPEKLKH WLPVDLYIGG VEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKVLFD LGVVPTKEPF QKLFNQGMIL GEGNEKMSKS KGNVINPDDI VKSHGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLD AAIAWSENGL DGSRRFLDRI WRLLITEDGS ISNKVVNNHS KALDKSYHQT 

       670        680        690        700        710        720 
VKKVTEDYNS LNFNTAISQL MVFINDCYKA EEIYKPYIEG FIKMLAPIAP HISEELWSRL 

       730        740        750        760        770        780 
GHDETITYQP WPSYDEALLV DDEIEIVVQV NGKVRAKINV SKDIAKEEME QIALDNEHVK 

       790        800 
SEIEGKDIKK VIAVPKKLVN IVAK 

« Hide

References

[1]"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008934 Genomic DNA. Translation: BAE18151.1.
RefSeqYP_301096.1. NC_007350.1.

3D structure databases

ProteinModelPortalQ49YI8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING342451.SSP1006.

Proteomic databases

PRIDEQ49YI8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE18151; BAE18151; SSP1006.
GeneID3615876.
KEGGssp:SSP1006.
PATRIC19623596. VBIStaSap90642_1005.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMANENGVEG.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSSAP342451:GKFA-1065-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_STAS1
AccessionPrimary (citable) accession number: Q49YI8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries