Reviewed, UniProtKB/Swiss-Prot Q49YD9 (DHA_STAS1)

Names and origin

Protein names

Recommended name:
    Alanine dehydrogenase
    EC=1.4.1.1

Gene names

Name:	ald
Ordered Locus Names:	SSP1057

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer By similarity.
Catalytic activity	L-alanine + H(2)O + NAD(+) = pyruvate + NH(3) + NADH.
Pathway	Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine dehydrogenase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Alanine dehydrogenase		PRO_0000287325
Regions
	Nucleotide binding	169 – 199	31	NAD By similarity
Sites
	Active site	95	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q49YD9-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 9BC859AAC3B4AF64	FASTA	371	39,903
	10 20 30 40 50 60 MIIGIPKEIK NNENRVSLSP SGVHALVEQG HTVIVEKSAG LGSYFEDVDY TEAGASIVNE 70 80 90 100 110 120 QAEVWNVDMV MKVKEPLEEE FQYFKEGLIL FTYLHLANEE KLTRALLENK VVGIAYETVQ 130 140 150 160 170 180 LPDRTLPLLT PMSEVAGRMS AQIGAEFLQK YKGGMGILLG GVPGVSKGRV SIIGGGQAGT 190 200 210 220 230 240 NAAKIALGLG ADVTILDVNP KRLQELEDLF DGRVHTIMSN PLNIEQCVKD SDLVIGAVLI 250 260 270 280 290 300 PGAKAPNLVT EDMVKEMRDG AVIVDIAIDQ GGIFETTDRI STHDDPTYKK HGVVHYAVAN 310 320 330 340 350 360 MPGAVPRTST IALNNATLPY AQQLASKGYL KALQDNHALS LGLNTINGEL TNKGVAEALN 370 LSYTDIESAL K

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE18202.1.
RefSeq	YP_301147.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3615425.
GenomeReviews	Gene locus SSP1057 in contig AP008934_GR.
KEGG	ssp:SSP1057.
NMPDR	fig|342451.4.peg.1117.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q49YD9.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP1057-MON.
Family and domain databases
InterPro	IPR007698. Ala_DHase/PNT_C. IPR008142. Ala_DHase/PNT_CS1. IPR008143. Ala_DHase/PNT_CS2. IPR007886. Ala_DHase/PNT_N. IPR008141. Ala_DHase_PNT. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00518. alaDH. 1 hit.
PROSITE	PS00836. ALADH_PNT_1. False negative. PS00837. ALADH_PNT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHA_STAS1

Accession

Primary (citable) accession number: Q49YD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)