Reviewed, UniProtKB/Swiss-Prot Q49YC7 (KPYK_STAS1)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40

Gene names

Name:	pyk
Ordered Locus Names:	SSP1069

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similarities	Belongs to the pyruvate kinase family. In the C-terminal section; belongs to the PEP-utilizing enzyme family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	Pyruvate kinase		PRO_0000294140
Sites
	Active site	220	1	By similarity
	Metal binding	222	1	Magnesium By similarity
	Metal binding	243	1	Magnesium By similarity
	Metal binding	244	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49YC7-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: FE093F0E7D948CAE	FASTA	586	62,853
	10 20 30 40 50 60 MRKTKIVCTI GPASESEEML EKLIKAGMNV ARLNFSHGDQ AEHKARIDTI RKVSKRLGKT 70 80 90 100 110 120 VAILLDTKGP EIRTHNMKDG LIELEKGSEV TVSMTEVEGT PEKFSVTYEN LINDVEEGSY 130 140 150 160 170 180 ILLDDGLIEL QVKSIDKANG EVLCDVLNTG ELKNKKGVNL PGVKVSLPGI TDKDADDINF 190 200 210 220 230 240 GISEGVDFIA ASFVRRPSDV LDIRKLLEAK QNKNISIIPK IENQEGIDNI KEILEVSDGL 250 260 270 280 290 300 MVARGDMGVE IPPESVPMVQ KDLIRQCNKL GKPVITATQM LDSMQRNPRA TRAEASDVAN 310 320 330 340 350 360 AIYDGTDAVM LSGETAAGQY PEEAVKTMRN IAVSAEAAQD YKKLLSDRTK LVETSLVNAI 370 380 390 400 410 420 GVSVAHTALN LNVKAIVAAT ESGSTARTIS KYRPQSDIIA VTPNAETARQ CALVWGIFPV 430 440 450 460 470 480 VKEGRKTTDA LLNNAVATAV ETERVQNGDL IIITAGVPTG EKGTTNMMKL HLVGDELAKG 490 500 510 520 530 540 QGIGRSSVVG KTLVVKDASE LEGKDLSESI IVTSSVDETL VPYIENAIGL ITEENGITSP 550 560 570 580 SAIIGLEKGI PTVVGVENAT SEIQSDVLIT VDANQGKIFE GYANVL

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE18214.1.
RefSeq	YP_301159.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3615437.
GenomeReviews	Gene locus SSP1069 in contig AP008934_GR.
KEGG	ssp:SSP1069.
NMPDR	fig|342451.4.peg.1129.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q49YC7.
OMA	Q49YC7. ATESGYT.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP1069-MON.
Family and domain databases
InterPro	IPR008279. PEP_mobile. IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.50.30.10. PEP_mobile. 1 hit. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00391. PEP-utilizers. 1 hit. PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_STAS1

Accession

Primary (citable) accession number: Q49YC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)