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Q49Y77 (Q49Y77_STAS1) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS002616

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS002616

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS002616

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS002616

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168 SAAS SAAS002616

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. HAMAP-Rule MF_00168 RuleBase RU003777

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site961Nucleophile By similarity HAMAP-Rule MF_00168
Metal binding3081Zinc By similarity HAMAP-Rule MF_00168
Metal binding3101Zinc By similarity HAMAP-Rule MF_00168
Metal binding3131Zinc By similarity HAMAP-Rule MF_00168
Metal binding3391Zinc By similarity HAMAP-Rule MF_00168
Binding site971Substrate By similarity HAMAP-Rule MF_00168

Sequences

Sequence LengthMass (Da)Tools
Q49Y77 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 5DE3C03A065A6CAC

FASTA38143,469
        10         20         30         40         50         60 
MPTPAVTYEH IKTCKQSGAR LGIVHTPHGS FETPMFMPVG TKATVKTMSP EELHQMETKI 

        70         80         90        100        110        120 
ILGNTYHLWL QPGNDIIRKS GGLHQFMNWN GPILTDSGGF QVFSLSNLRK ITEEGVEFRH 

       130        140        150        160        170        180 
HTNGSKLFLS PEKSMQIQND LGSDIMMAFD ECPPMPAEYK YVKDSIERTT RWAERCLKAH 

       190        200        210        220        230        240 
SRPEDQALFG IIQGGEYKDL RKQSAEELVA LDFPGYAIGG LSVGEPKPVM YDMVHHTEQF 

       250        260        270        280        290        300 
MPKDKPRYLM GVGSPDALIE CSIRGMDMFD CVLPTRIARN GTCMTSHGRV IIKNAKYAED 

       310        320        330        340        350        360 
LGPLDDNCDC YTCKNYSRAY IRHLIKAEET FGIRLTTYHN LHFLLKLMEN IRQAIREDRL 

       370        380 
LDFKEEFFEQ YGLNVENPKN F 

« Hide

References

[1]"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008934 Genomic DNA. Translation: BAE18265.1.
RefSeqYP_301210.1. NC_007350.1.

3D structure databases

ProteinModelPortalQ49Y77.
SMRQ49Y77. Positions 7-372.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING342451.SSP1120.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE18265; BAE18265; SSP1120.
GeneID3614962.
KEGGssp:SSP1120.
PATRIC19623824. VBIStaSap90642_1119.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMATPRFMPV.
OrthoDBEOG6SNDVG.
ProtClustDBPRK00112.

Enzyme and pathway databases

BioCycSSAP342451:GKFA-1179-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. SSF51713. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ49Y77_STAS1
AccessionPrimary (citable) accession number: Q49Y77
Entry history
Integrated into UniProtKB/TrEMBL: September 13, 2005
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)