Reviewed, UniProtKB/Swiss-Prot Q49XM6 (ARLS_STAS1)

Names and origin

Protein names

Recommended name:
    Signal transduction histidine-protein kinase arlS
    EC=2.7.13.3

Gene names

Name:	arlS
Ordered Locus Names:	SSP1324

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of the two-component regulatory system arlS/arlR. ArlS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to arlR By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	Signal transduction histidine-protein kinase arlS		PRO_0000293452
Regions
	Transmembrane	12 – 32	21	Potential
	Transmembrane	156 – 176	21	Potential
	Domain	178 – 231	54	HAMP
	Domain	239 – 451	213	Histidine kinase
Amino acid modifications
	Modified residue	242	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49XM6-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 0750F2CF493D1AC8	FASTA	451	52,423
	10 20 30 40 50 60 MKQRKLKTKW MLITTTITFL TIFLFSIIII FFLSNSLRHN EVDEAERSSE DIVKLFESKQ 70 80 90 100 110 120 IEHVTPLDLN ASLGNFQKVM LFNEDGHKLM ETSNDNSITF SPNIVPTNVN RIAVKSDHKK 130 140 150 160 170 180 DYLIITDHIE SPKFNGYSVI VHSLEDYKAL VNSLYFIALI FGVIATFITA IISYFFSSQI 190 200 210 220 230 240 TKPLILMSNK MQQIRRDGFQ EKVELSTNYE ETDNLIVTFN EMMLQLEESF NQQRQFVEDA 250 260 270 280 290 300 SHELRTPLQI IQGHLNLINR WGKKDAAILE ESLDISLEEM TRITKLVEEL LLLTKDNNNS 310 320 330 340 350 360 RDGEIENVEI NQEIASRIKS LSQLHSDYTF EFDAFPKPLN IKIDRYQFEQ MLIIFIDNAM 370 380 390 400 410 420 KYDQINKYIQ IQTKLRNKQI SIEITDHGVG IPKEDIEFIF DRFYRVDKSR SRKLGGNGLG 430 440 450 LSIAKKIIEL NNGTIHVDSE VDKYTTFKIT F

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE18469.1.
RefSeq	YP_301414.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q49XM6.
Genome annotation databases
GeneID	3616662.
GenomeReviews	Gene locus SSP1324 in contig AP008934_GR.
KEGG	ssp:SSP1324.
NMPDR	fig|342451.4.peg.604.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q49XM6.
OMA	FIDNAMK.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP1324-MON.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLS_STAS1

Accession

Primary (citable) accession number: Q49XM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)