Q49XD2 (GUAC_STAS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP reductase
EC=1.7.1.7

Alternative name(s):
Guanosine 5'-monophosphate oxidoreductase
Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	SSP1420

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	GMP reductase HAMAP MF_01511		PRO_0000093772
Regions
	Nucleotide binding	203 – 226	24	NADP Potential
Sites
	Active site	174	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49XD2 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 420B00BC5727C221	FASTA	328	36,408
	10 20 30 40 50 60 MKIFDYEDIQ LVPNKCIVNS RSECDTTIQF GPRSFKLPVV PANMQTVMNE TLAEWFAEND 70 80 90 100 110 120 YFYIMHRFDE EGRIPFIKKM QEKGLFASIS VGVKEREFGF VESLAAENVI PEYITIDIAH 130 140 150 160 170 180 GHSDSVINMI KHIKKHIPEV FVIAGNVGTP EGVRELENAG ADATKVGIGP GRVCITKIKT 190 200 210 220 230 240 GFGTGGWQLS ALNHCSKAAR KPIIADGGIR THGDIAKSIR FGASMVMVGS LFAAHEESPG 250 260 270 280 290 300 ETVELDGKMY KEYFGSASEF QKGEHKNVEG KKMFVEHKGS LFNTLTEMQQ DLQSSISYAG 310 320 GKDLNSLRKV DYVIVRNSIF NGDRDQNL

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008934 Genomic DNA. Translation: BAE18565.1.
RefSeq	YP_301510.1. NC_007350.1.
3D structure databases
ProteinModelPortal	Q49XD2.
SMR	Q49XD2. Positions 3-320.
ModBase	Search...
Protein-protein interaction databases
STRING	Q49XD2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000048199; EBSTAP00000046568; EBSTAG00000048196.
GeneID	3615359.
GenomeReviews	Gene locus SSP1420 in contig AP008934_GR.
KEGG	ssp:SSP1420.
NMPDR	fig|342451.4.peg.775.
PATRIC	19624434. VBIStaSap90642_1424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0516.
GeneTree	EBGT00050000024354.
HOGENOM	HBG298985.
OMA	PDYITID.
PhylomeDB	Q49XD2.
ProtClustDB	PRK05458.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP1420-MONOMER.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00364.
PANTHER	PTHR11911:SF30. PTHR11911:SF30. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_STAS1

Accession

Primary (citable) accession number: Q49XD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program