Q49X35 (TOP1_STAS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme

Gene names

Name:	topA
Ordered Locus Names:	SSP1518

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 688	688	DNA topoisomerase 1		PRO_0000285947
Regions
	Domain	3 – 112	110	Toprim
	Zinc finger	576 – 602	27	C4-type 1
	Zinc finger	616 – 644	29	C4-type 2
	Zinc finger	657 – 680	24	C4-type 3
Sites
	Active site	298	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49X35 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 24BC79B6DD63D72B	FASTA	688	79,130
	10 20 30 40 50 60 MAENLVIVES PAKAKTIEKY LGKKYKVIAS MGHVRDLPRS QMGVDAENDY EPKYITIRGK 70 80 90 100 110 120 GPVVKELKKH AKKAKKVFLA SDPDREGEAI AWHLANILNL EDSTENRVVF NEITKDAVKD 130 140 150 160 170 180 SFKHPRGIEM ELVDAQQARR ILDRLVGYNI SPVLWKKVKK GLSAGRVQSV ALRLVIDREN 190 200 210 220 230 240 EIRNFKPEEY WKIEGEFRYK KTKFTAKFLH FKNKPFKLTE KADVEKITTQ LDGDQFEVTK 250 260 270 280 290 300 VTKKEKTRYP ANPFTTSTLQ QEAARKLNFK ARKTMMLAQQ LYEGIDLKKQ GTVGLITYMR 310 320 330 340 350 360 TDSTRISDQA QSEAKNYIQE TYGNDYTSNR KSKGQGDQDA HEAIRPSSTL RTPNEMKNFL 370 380 390 400 410 420 TRDQHRLYKL IWERFVASQM APAILDTVAM DLTQNDIKFR ANGQTIKFKG FMTLYVETKD 430 440 450 460 470 480 DSEDGKDNKL PNIGEGEMVT ATNIEPSQHF TQPPPRYTEA RLVKTMEELK IGRPSTYAPT 490 500 510 520 530 540 IDTIQKRNYV KNESKRFVPT ELGEIVHEQV KDYFPEIIDV DFTVNMETLL DKVADGEIGW 550 560 570 580 590 600 KKVISDFYSS FKQDVERAEE EMEKIEIKDE PAGEDCEVCG SPMVIKMGRY GKFMACSNFP 610 620 630 640 650 660 DCRNTKAIVK TIGVTCPKCK EGDVVERKSK KNRIFYGCSK YPECDFVTWD KPIGRDCPKC 670 680 EHYLVEKKQG RKSQVVCSNC DYKEEEQK

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008934 Genomic DNA. Translation: BAE18663.1.
RefSeq	YP_301608.1. NC_007350.1.
3D structure databases
HSSP	HSSP built from PDB template 2GAI based on UniProtKB P46799.
ProteinModelPortal	Q49X35.
ModBase	Search...
Protein-protein interaction databases
STRING	Q49X35.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000048517; EBSTAP00000046886; EBSTAG00000048514.
GeneID	3615164.
GenomeReviews	Gene locus SSP1518 in contig AP008934_GR.
KEGG	ssp:SSP1518.
NMPDR	fig|342451.4.peg.1534.
PATRIC	19624626. VBIStaSap90642_1520.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00050000024420.
HOGENOM	HBG512935.
OMA	EEYWSID.
PhylomeDB	Q49X35.
ProtClustDB	PRK05582.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP1518-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 3 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_STAS1

Accession

Primary (citable) accession number: Q49X35

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program