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Q49X35

- TOP1_STAS1

UniProt

Q49X35 - TOP1_STAS1

Protein

DNA topoisomerase 1

Gene

topA

Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
    Sitei33 – 331Interaction with DNAUniRule annotation
    Metal bindingi82 – 821Magnesium 1; catalyticUniRule annotation
    Metal bindingi82 – 821Magnesium 2UniRule annotation
    Metal bindingi84 – 841Magnesium 2UniRule annotation
    Sitei139 – 1391Interaction with DNAUniRule annotation
    Sitei140 – 1401Interaction with DNAUniRule annotation
    Sitei143 – 1431Interaction with DNAUniRule annotation
    Sitei148 – 1481Interaction with DNAUniRule annotation
    Sitei155 – 1551Interaction with DNAUniRule annotation
    Active sitei298 – 2981O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei300 – 3001Interaction with DNAUniRule annotation
    Sitei487 – 4871Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri576 – 60227C4-type 1Add
    BLAST
    Zinc fingeri616 – 64429C4-type 2Add
    BLAST
    Zinc fingeri657 – 68024C4-type 3Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciSSAP342451:GKFA-1577-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:SSP1518
    OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
    Taxonomic identifieri342451 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000006371: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 688688DNA topoisomerase 1PRO_0000285947Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi342451.SSP1518.

    Structurei

    3D structure databases

    ProteinModelPortaliQ49X35.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 113111ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni163 – 1686Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri576 – 60227C4-type 1Add
    BLAST
    Zinc fingeri616 – 64429C4-type 2Add
    BLAST
    Zinc fingeri657 – 68024C4-type 3Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0551.
    HOGENOMiHOG000004018.
    KOiK03168.
    OMAiWINKAKK.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.460.10. 3 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q49X35-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAENLVIVES PAKAKTIEKY LGKKYKVIAS MGHVRDLPRS QMGVDAENDY    50
    EPKYITIRGK GPVVKELKKH AKKAKKVFLA SDPDREGEAI AWHLANILNL 100
    EDSTENRVVF NEITKDAVKD SFKHPRGIEM ELVDAQQARR ILDRLVGYNI 150
    SPVLWKKVKK GLSAGRVQSV ALRLVIDREN EIRNFKPEEY WKIEGEFRYK 200
    KTKFTAKFLH FKNKPFKLTE KADVEKITTQ LDGDQFEVTK VTKKEKTRYP 250
    ANPFTTSTLQ QEAARKLNFK ARKTMMLAQQ LYEGIDLKKQ GTVGLITYMR 300
    TDSTRISDQA QSEAKNYIQE TYGNDYTSNR KSKGQGDQDA HEAIRPSSTL 350
    RTPNEMKNFL TRDQHRLYKL IWERFVASQM APAILDTVAM DLTQNDIKFR 400
    ANGQTIKFKG FMTLYVETKD DSEDGKDNKL PNIGEGEMVT ATNIEPSQHF 450
    TQPPPRYTEA RLVKTMEELK IGRPSTYAPT IDTIQKRNYV KNESKRFVPT 500
    ELGEIVHEQV KDYFPEIIDV DFTVNMETLL DKVADGEIGW KKVISDFYSS 550
    FKQDVERAEE EMEKIEIKDE PAGEDCEVCG SPMVIKMGRY GKFMACSNFP 600
    DCRNTKAIVK TIGVTCPKCK EGDVVERKSK KNRIFYGCSK YPECDFVTWD 650
    KPIGRDCPKC EHYLVEKKQG RKSQVVCSNC DYKEEEQK 688
    Length:688
    Mass (Da):79,130
    Last modified:September 13, 2005 - v1
    Checksum:i24BC79B6DD63D72B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008934 Genomic DNA. Translation: BAE18663.1.
    RefSeqiYP_301608.1. NC_007350.1.

    Genome annotation databases

    EnsemblBacteriaiBAE18663; BAE18663; SSP1518.
    GeneIDi3615164.
    KEGGissp:SSP1518.
    PATRICi19624626. VBIStaSap90642_1520.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008934 Genomic DNA. Translation: BAE18663.1 .
    RefSeqi YP_301608.1. NC_007350.1.

    3D structure databases

    ProteinModelPortali Q49X35.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 342451.SSP1518.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAE18663 ; BAE18663 ; SSP1518 .
    GeneIDi 3615164.
    KEGGi ssp:SSP1518.
    PATRICi 19624626. VBIStaSap90642_1520.

    Phylogenomic databases

    eggNOGi COG0551.
    HOGENOMi HOG000004018.
    KOi K03168.
    OMAi WINKAKK.
    OrthoDBi EOG6S7XQ9.

    Enzyme and pathway databases

    BioCyci SSAP342451:GKFA-1577-MONOMER.

    Family and domain databases

    Gene3Di 1.10.460.10. 3 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
      Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
      Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15305 / DSM 20229.

    Entry informationi

    Entry nameiTOP1_STAS1
    AccessioniPrimary (citable) accession number: Q49X35
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3