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Q49X35 (TOP1_STAS1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:SSP1518
OrganismStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]
Taxonomic identifier342451 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length688 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 688688DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000285947

Regions

Domain3 – 113111Toprim
Zinc finger576 – 60227C4-type 1 HAMAP-Rule MF_00952
Zinc finger616 – 64429C4-type 2 HAMAP-Rule MF_00952
Zinc finger657 – 68024C4-type 3 HAMAP-Rule MF_00952
Region163 – 1686Interaction with DNA By similarity

Sites

Active site2981O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium 1; catalytic By similarity
Metal binding821Magnesium 1; catalytic By similarity
Metal binding821Magnesium 2 By similarity
Metal binding841Magnesium 2 By similarity
Site331Interaction with DNA By similarity
Site1391Interaction with DNA By similarity
Site1401Interaction with DNA By similarity
Site1431Interaction with DNA By similarity
Site1481Interaction with DNA By similarity
Site1551Interaction with DNA By similarity
Site3001Interaction with DNA By similarity
Site4871Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q49X35 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 24BC79B6DD63D72B

FASTA68879,130
        10         20         30         40         50         60 
MAENLVIVES PAKAKTIEKY LGKKYKVIAS MGHVRDLPRS QMGVDAENDY EPKYITIRGK 

        70         80         90        100        110        120 
GPVVKELKKH AKKAKKVFLA SDPDREGEAI AWHLANILNL EDSTENRVVF NEITKDAVKD 

       130        140        150        160        170        180 
SFKHPRGIEM ELVDAQQARR ILDRLVGYNI SPVLWKKVKK GLSAGRVQSV ALRLVIDREN 

       190        200        210        220        230        240 
EIRNFKPEEY WKIEGEFRYK KTKFTAKFLH FKNKPFKLTE KADVEKITTQ LDGDQFEVTK 

       250        260        270        280        290        300 
VTKKEKTRYP ANPFTTSTLQ QEAARKLNFK ARKTMMLAQQ LYEGIDLKKQ GTVGLITYMR 

       310        320        330        340        350        360 
TDSTRISDQA QSEAKNYIQE TYGNDYTSNR KSKGQGDQDA HEAIRPSSTL RTPNEMKNFL 

       370        380        390        400        410        420 
TRDQHRLYKL IWERFVASQM APAILDTVAM DLTQNDIKFR ANGQTIKFKG FMTLYVETKD 

       430        440        450        460        470        480 
DSEDGKDNKL PNIGEGEMVT ATNIEPSQHF TQPPPRYTEA RLVKTMEELK IGRPSTYAPT 

       490        500        510        520        530        540 
IDTIQKRNYV KNESKRFVPT ELGEIVHEQV KDYFPEIIDV DFTVNMETLL DKVADGEIGW 

       550        560        570        580        590        600 
KKVISDFYSS FKQDVERAEE EMEKIEIKDE PAGEDCEVCG SPMVIKMGRY GKFMACSNFP 

       610        620        630        640        650        660 
DCRNTKAIVK TIGVTCPKCK EGDVVERKSK KNRIFYGCSK YPECDFVTWD KPIGRDCPKC 

       670        680 
EHYLVEKKQG RKSQVVCSNC DYKEEEQK 

« Hide

References

[1]"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008934 Genomic DNA. Translation: BAE18663.1.
RefSeqYP_301608.1. NC_007350.1.

3D structure databases

ProteinModelPortalQ49X35.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING342451.SSP1518.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE18663; BAE18663; SSP1518.
GeneID3615164.
KEGGssp:SSP1518.
PATRIC19624626. VBIStaSap90642_1520.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004018.
KOK03168.
OMAMVYKMGR.
OrthoDBEOG6S7XQ9.
ProtClustDBPRK05582.

Enzyme and pathway databases

BioCycSSAP342451:GKFA-1577-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_STAS1
AccessionPrimary (citable) accession number: Q49X35
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families