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Q49WX5

- SYI_STAS1

UniProt

Q49WX5 - SYI_STAS1

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
    Binding sitei598 – 5981ATPUniRule annotation
    Metal bindingi885 – 8851ZincUniRule annotation
    Metal bindingi888 – 8881ZincUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSSAP342451:GKFA-1637-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:SSP1578
    OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
    Taxonomic identifieri342451 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000006371: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 916916Isoleucine--tRNA ligasePRO_0000098474Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi342451.SSP1578.

    Structurei

    3D structure databases

    ProteinModelPortaliQ49WX5.
    SMRiQ49WX5. Positions 1-916.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi595 – 5995"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGRRSCQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q49WX5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKDTLLMP KTDFPMRGGL PNKEPKIQEE WDAKNIYQKV LDKNEGNPSF    50
    ILHDGPPYAN GNLHMGHALN KILKDIITRY KSMLGYYAPY VPGWDTHGLP 100
    IEQALTKKGV KRKELSIAEF RKKCEAFALE QIDNQKKDFK RLGVKGDFNN 150
    PYITLKPEYE AAQIRLFGEM ADKGLIYKGK KPVYWSPSSE SSLAEAEIEY 200
    QDKRSPSIYV AFDVIDGKGI VDDDAQFIIW TTTPWTLPSN VAITVHPDLT 250
    YGQYNVNGKK YIIGKDLASD VAEALDWDED TLELEKEFKG KDLEYIKAQH 300
    PFFDRESLVI NGLHVTTDAG TGCVHTAPGH GEDDYIVGQK YNLPVISPVD 350
    DKGVFTDEAG QFEGMFYDKA NKEITDLLKE DGSLLKLEFI THSYPHDWRT 400
    KKPVIFRATP QWFASIDKVR EDILSAIDDT QFKVDWGKTR IYNMIRDRGE 450
    WVISRQRVWG VPLPVFYAEN GDIIMTSETV NHVADLFEAN GSNIWFEREA 500
    KDLLPEGFTH PGSPNGEFTK ETDIMDVWFD SGSSHRGVLE ARPELSYPAD 550
    LYLEGSDQYR GWFNSSITTS VATRGQSPYK MLLSHGFVMD GEGKKMSKSL 600
    GNVIVPDQIV KQKGADIARL WVSSVDYLAD VRISDEILKQ SSDVYRKIRN 650
    TLRFMLGNVS DYNPATDAIA EKDLLEVDKY LLNRLREFTA NTLDHYDNYD 700
    YLDIYQEVQN FINVELSNFY LDYGKDILYI EERDSHKRRS MQTVLYQIVV 750
    DMTKLLAPIL VHTAEEVWSH IPHVEEESVH LTNMPERVEI DQAFVDRWNT 800
    FMKLRDDVNR ALEVARNEKV IGKSLEAKVV IGSNENFDAT TFLQQFKDLQ 850
    QLFITSQAEV VDKVDDGVAY QHGDIRIEHA HGEKCERCWN YSEELGSVGE 900
    LDNLCPRCQA VVKTLV 916
    Length:916
    Mass (Da):104,782
    Last modified:September 13, 2005 - v1
    Checksum:i74E0CF2322EA81CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008934 Genomic DNA. Translation: BAE18723.1.
    RefSeqiWP_011303319.1. NC_007350.1.
    YP_301668.1. NC_007350.1.

    Genome annotation databases

    EnsemblBacteriaiBAE18723; BAE18723; SSP1578.
    GeneIDi3615322.
    KEGGissp:SSP1578.
    PATRICi19624746. VBIStaSap90642_1580.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008934 Genomic DNA. Translation: BAE18723.1 .
    RefSeqi WP_011303319.1. NC_007350.1.
    YP_301668.1. NC_007350.1.

    3D structure databases

    ProteinModelPortali Q49WX5.
    SMRi Q49WX5. Positions 1-916.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 342451.SSP1578.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAE18723 ; BAE18723 ; SSP1578 .
    GeneIDi 3615322.
    KEGGi ssp:SSP1578.
    PATRICi 19624746. VBIStaSap90642_1580.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGRRSCQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SSAP342451:GKFA-1637-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
      Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
      Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15305 / DSM 20229.

    Entry informationi

    Entry nameiSYI_STAS1
    AccessioniPrimary (citable) accession number: Q49WX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3