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Q49WX5

- SYI_STAS1

UniProt

Q49WX5 - SYI_STAS1

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation
Metal bindingi885 – 8851ZincUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSAP342451:GKFA-1637-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SSP1578
OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Taxonomic identifieri342451 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000006371: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 916916Isoleucine--tRNA ligasePRO_0000098474Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi342451.SSP1578.

Structurei

3D structure databases

ProteinModelPortaliQ49WX5.
SMRiQ49WX5. Positions 1-916.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q49WX5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKDTLLMP KTDFPMRGGL PNKEPKIQEE WDAKNIYQKV LDKNEGNPSF
60 70 80 90 100
ILHDGPPYAN GNLHMGHALN KILKDIITRY KSMLGYYAPY VPGWDTHGLP
110 120 130 140 150
IEQALTKKGV KRKELSIAEF RKKCEAFALE QIDNQKKDFK RLGVKGDFNN
160 170 180 190 200
PYITLKPEYE AAQIRLFGEM ADKGLIYKGK KPVYWSPSSE SSLAEAEIEY
210 220 230 240 250
QDKRSPSIYV AFDVIDGKGI VDDDAQFIIW TTTPWTLPSN VAITVHPDLT
260 270 280 290 300
YGQYNVNGKK YIIGKDLASD VAEALDWDED TLELEKEFKG KDLEYIKAQH
310 320 330 340 350
PFFDRESLVI NGLHVTTDAG TGCVHTAPGH GEDDYIVGQK YNLPVISPVD
360 370 380 390 400
DKGVFTDEAG QFEGMFYDKA NKEITDLLKE DGSLLKLEFI THSYPHDWRT
410 420 430 440 450
KKPVIFRATP QWFASIDKVR EDILSAIDDT QFKVDWGKTR IYNMIRDRGE
460 470 480 490 500
WVISRQRVWG VPLPVFYAEN GDIIMTSETV NHVADLFEAN GSNIWFEREA
510 520 530 540 550
KDLLPEGFTH PGSPNGEFTK ETDIMDVWFD SGSSHRGVLE ARPELSYPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSITTS VATRGQSPYK MLLSHGFVMD GEGKKMSKSL
610 620 630 640 650
GNVIVPDQIV KQKGADIARL WVSSVDYLAD VRISDEILKQ SSDVYRKIRN
660 670 680 690 700
TLRFMLGNVS DYNPATDAIA EKDLLEVDKY LLNRLREFTA NTLDHYDNYD
710 720 730 740 750
YLDIYQEVQN FINVELSNFY LDYGKDILYI EERDSHKRRS MQTVLYQIVV
760 770 780 790 800
DMTKLLAPIL VHTAEEVWSH IPHVEEESVH LTNMPERVEI DQAFVDRWNT
810 820 830 840 850
FMKLRDDVNR ALEVARNEKV IGKSLEAKVV IGSNENFDAT TFLQQFKDLQ
860 870 880 890 900
QLFITSQAEV VDKVDDGVAY QHGDIRIEHA HGEKCERCWN YSEELGSVGE
910
LDNLCPRCQA VVKTLV
Length:916
Mass (Da):104,782
Last modified:September 13, 2005 - v1
Checksum:i74E0CF2322EA81CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA. Translation: BAE18723.1.
RefSeqiYP_301668.1. NC_007350.1.

Genome annotation databases

EnsemblBacteriaiBAE18723; BAE18723; SSP1578.
GeneIDi3615322.
KEGGissp:SSP1578.
PATRICi19624746. VBIStaSap90642_1580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA. Translation: BAE18723.1 .
RefSeqi YP_301668.1. NC_007350.1.

3D structure databases

ProteinModelPortali Q49WX5.
SMRi Q49WX5. Positions 1-916.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 342451.SSP1578.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAE18723 ; BAE18723 ; SSP1578 .
GeneIDi 3615322.
KEGGi ssp:SSP1578.
PATRICi 19624746. VBIStaSap90642_1580.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SSAP342451:GKFA-1637-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
    Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
    Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15305 / DSM 20229.

Entry informationi

Entry nameiSYI_STAS1
AccessioniPrimary (citable) accession number: Q49WX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3