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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei554Aminoacyl-adenylateUniRule annotation1
Binding sitei598ATPUniRule annotation1
Metal bindingi885ZincUniRule annotation1
Metal bindingi888ZincUniRule annotation1
Metal bindingi905ZincUniRule annotation1
Metal bindingi908ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSAP342451:G1G2H-1593-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SSP1578
OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Taxonomic identifieri342451 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006371 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984741 – 916Isoleucine--tRNA ligaseAdd BLAST916

Proteomic databases

PRIDEiQ49WX5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi342451.SSP1578

Structurei

3D structure databases

ProteinModelPortaliQ49WX5
SMRiQ49WX5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi595 – 599"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q49WX5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKDTLLMP KTDFPMRGGL PNKEPKIQEE WDAKNIYQKV LDKNEGNPSF
60 70 80 90 100
ILHDGPPYAN GNLHMGHALN KILKDIITRY KSMLGYYAPY VPGWDTHGLP
110 120 130 140 150
IEQALTKKGV KRKELSIAEF RKKCEAFALE QIDNQKKDFK RLGVKGDFNN
160 170 180 190 200
PYITLKPEYE AAQIRLFGEM ADKGLIYKGK KPVYWSPSSE SSLAEAEIEY
210 220 230 240 250
QDKRSPSIYV AFDVIDGKGI VDDDAQFIIW TTTPWTLPSN VAITVHPDLT
260 270 280 290 300
YGQYNVNGKK YIIGKDLASD VAEALDWDED TLELEKEFKG KDLEYIKAQH
310 320 330 340 350
PFFDRESLVI NGLHVTTDAG TGCVHTAPGH GEDDYIVGQK YNLPVISPVD
360 370 380 390 400
DKGVFTDEAG QFEGMFYDKA NKEITDLLKE DGSLLKLEFI THSYPHDWRT
410 420 430 440 450
KKPVIFRATP QWFASIDKVR EDILSAIDDT QFKVDWGKTR IYNMIRDRGE
460 470 480 490 500
WVISRQRVWG VPLPVFYAEN GDIIMTSETV NHVADLFEAN GSNIWFEREA
510 520 530 540 550
KDLLPEGFTH PGSPNGEFTK ETDIMDVWFD SGSSHRGVLE ARPELSYPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSITTS VATRGQSPYK MLLSHGFVMD GEGKKMSKSL
610 620 630 640 650
GNVIVPDQIV KQKGADIARL WVSSVDYLAD VRISDEILKQ SSDVYRKIRN
660 670 680 690 700
TLRFMLGNVS DYNPATDAIA EKDLLEVDKY LLNRLREFTA NTLDHYDNYD
710 720 730 740 750
YLDIYQEVQN FINVELSNFY LDYGKDILYI EERDSHKRRS MQTVLYQIVV
760 770 780 790 800
DMTKLLAPIL VHTAEEVWSH IPHVEEESVH LTNMPERVEI DQAFVDRWNT
810 820 830 840 850
FMKLRDDVNR ALEVARNEKV IGKSLEAKVV IGSNENFDAT TFLQQFKDLQ
860 870 880 890 900
QLFITSQAEV VDKVDDGVAY QHGDIRIEHA HGEKCERCWN YSEELGSVGE
910
LDNLCPRCQA VVKTLV
Length:916
Mass (Da):104,782
Last modified:September 13, 2005 - v1
Checksum:i74E0CF2322EA81CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA Translation: BAE18723.1
RefSeqiWP_011303319.1, NZ_MTGA01000034.1

Genome annotation databases

EnsemblBacteriaiBAE18723; BAE18723; SSP1578
GeneIDi3615322
KEGGissp:SSP1578
PATRICifig|342451.11.peg.1580

Similar proteinsi

Entry informationi

Entry nameiSYI_STAS1
AccessioniPrimary (citable) accession number: Q49WX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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