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Q49WX5

- SYI_STAS1

UniProt

Q49WX5 - SYI_STAS1

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Protein
Isoleucine--tRNA ligase
Gene
ileS, SSP1578
Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylate By similarity
Binding sitei598 – 5981ATP By similarity
Metal bindingi885 – 8851Zinc By similarity
Metal bindingi888 – 8881Zinc By similarity
Metal bindingi905 – 9051Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSAP342451:GKFA-1637-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SSP1578
OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Taxonomic identifieri342451 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000006371: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 916916Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098474Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi342451.SSP1578.

Structurei

3D structure databases

ProteinModelPortaliQ49WX5.
SMRiQ49WX5. Positions 1-916.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi595 – 5995"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q49WX5-1 [UniParc]FASTAAdd to Basket

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MDYKDTLLMP KTDFPMRGGL PNKEPKIQEE WDAKNIYQKV LDKNEGNPSF    50
ILHDGPPYAN GNLHMGHALN KILKDIITRY KSMLGYYAPY VPGWDTHGLP 100
IEQALTKKGV KRKELSIAEF RKKCEAFALE QIDNQKKDFK RLGVKGDFNN 150
PYITLKPEYE AAQIRLFGEM ADKGLIYKGK KPVYWSPSSE SSLAEAEIEY 200
QDKRSPSIYV AFDVIDGKGI VDDDAQFIIW TTTPWTLPSN VAITVHPDLT 250
YGQYNVNGKK YIIGKDLASD VAEALDWDED TLELEKEFKG KDLEYIKAQH 300
PFFDRESLVI NGLHVTTDAG TGCVHTAPGH GEDDYIVGQK YNLPVISPVD 350
DKGVFTDEAG QFEGMFYDKA NKEITDLLKE DGSLLKLEFI THSYPHDWRT 400
KKPVIFRATP QWFASIDKVR EDILSAIDDT QFKVDWGKTR IYNMIRDRGE 450
WVISRQRVWG VPLPVFYAEN GDIIMTSETV NHVADLFEAN GSNIWFEREA 500
KDLLPEGFTH PGSPNGEFTK ETDIMDVWFD SGSSHRGVLE ARPELSYPAD 550
LYLEGSDQYR GWFNSSITTS VATRGQSPYK MLLSHGFVMD GEGKKMSKSL 600
GNVIVPDQIV KQKGADIARL WVSSVDYLAD VRISDEILKQ SSDVYRKIRN 650
TLRFMLGNVS DYNPATDAIA EKDLLEVDKY LLNRLREFTA NTLDHYDNYD 700
YLDIYQEVQN FINVELSNFY LDYGKDILYI EERDSHKRRS MQTVLYQIVV 750
DMTKLLAPIL VHTAEEVWSH IPHVEEESVH LTNMPERVEI DQAFVDRWNT 800
FMKLRDDVNR ALEVARNEKV IGKSLEAKVV IGSNENFDAT TFLQQFKDLQ 850
QLFITSQAEV VDKVDDGVAY QHGDIRIEHA HGEKCERCWN YSEELGSVGE 900
LDNLCPRCQA VVKTLV 916
Length:916
Mass (Da):104,782
Last modified:September 13, 2005 - v1
Checksum:i74E0CF2322EA81CC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008934 Genomic DNA. Translation: BAE18723.1.
RefSeqiWP_011303319.1. NC_007350.1.
YP_301668.1. NC_007350.1.

Genome annotation databases

EnsemblBacteriaiBAE18723; BAE18723; SSP1578.
GeneIDi3615322.
KEGGissp:SSP1578.
PATRICi19624746. VBIStaSap90642_1580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008934 Genomic DNA. Translation: BAE18723.1 .
RefSeqi WP_011303319.1. NC_007350.1.
YP_301668.1. NC_007350.1.

3D structure databases

ProteinModelPortali Q49WX5.
SMRi Q49WX5. Positions 1-916.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 342451.SSP1578.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAE18723 ; BAE18723 ; SSP1578 .
GeneIDi 3615322.
KEGGi ssp:SSP1578.
PATRICi 19624746. VBIStaSap90642_1580.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SSAP342451:GKFA-1637-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
    Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
    Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15305 / DSM 20229.

Entry informationi

Entry nameiSYI_STAS1
AccessioniPrimary (citable) accession number: Q49WX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: September 3, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi