Q49WJ5 (PUR5_STAS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	SSP1719

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus saprophyticus › Staphylococcus saprophyticus subsp. saprophyticus

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741		PRO_0000148254

Sequences

	Sequence		Length	Mass (Da)
	Q49WJ5 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 437494CFB7529ED7	FASTA	342	37,005
	10 20 30 40 50 60 MSKAYQQAGV DINAGYEAVE RMTSHVQRTM RKEVLGGLGG FGATFDLSQL NMKAPLLVSG 70 80 90 100 110 120 TDGVGTKLKL AIDNDKHDTI GIDAVAMCVN DILTTGAEPL YFLDYIATNK VVPEVIEQIV 130 140 150 160 170 180 KGVSDGCEET NTALIGGETA EMGEMYHEGE YDLAGFAVGA VEKDDYIDGS SVEPGQAIIG 190 200 210 220 230 240 LESSGIHSNG YSLVRKLIQQ SGIKLSEPFN QEQTFLDAFL KPTRLYVKPV LAVKSSIKIY 250 260 270 280 290 300 AMTHITGGGF YENIPRALPE GITAKIDVTQ FPTPAIFDWL QEQGNISTDE MYNIFNMGIG 310 320 330 340 FTLVVDNNQV ESTLEILNGQ NIKAYKIGET VKGNEPIQLT GV

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008934 Genomic DNA. Translation: BAE18864.1.
RefSeq	YP_301809.1. NC_007350.1.
3D structure databases
ProteinModelPortal	Q49WJ5.
SMR	Q49WJ5. Positions 14-334.
ModBase	Search...
Protein-protein interaction databases
STRING	342451.SSP1719.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAE18864; BAE18864; SSP1719.
GeneID	3616628.
KEGG	ssp:SSP1719.
PATRIC	19625025. VBIStaSap90642_1718.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HOG000229090.
KO	K01933.
OMA	IDMIAMN.
ProtClustDB	PRK05385.
Enzyme and pathway databases
UniPathway	UPA00074; UER00129.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_STAS1

Accession

Primary (citable) accession number: Q49WJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program