Reviewed, UniProtKB/Swiss-Prot Q49WI9 (PURK_STAS1)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	SSP1725

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Phosphoribosylaminoimidazole carboxylase ATPase subunit		PRO_0000075012
Regions
	Domain	112 – 296	185	ATP-grasp
	Nucleotide binding	183 – 186	4	ATP By similarity
	Nucleotide binding	268 – 269	2	ATP By similarity
Sites
	Binding site	108	1	ATP By similarity
	Binding site	148	1	ATP By similarity
	Binding site	159	1	ATP By similarity
	Binding site	191	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49WI9-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: B3EEF6B40DDF1D64	FASTA	375	42,476
	10 20 30 40 50 60 MNLSKLKFGS TIGIIGGGQL GKMMAQSAQQ MGYKVIVLDP NSSCPCQYVA NEFINAAYDD 70 80 90 100 110 120 QIALDRLGAL SDVITYEFEN ISANQLQALA SKYNIPQGYQ AIQLLQDRLT EKQTLQDSGS 130 140 150 160 170 180 NIAPFLQLTD YDDLVKAVDS IGYPFIVKTR FGGYDGKGQV LVKDATHLDE ARQLIQAQEC 190 200 210 220 230 240 VAEQYLNLSK EVSLTVTIGT NNQITYFPLQ ENEHQNQILF KTIVPARVQD NKEQEARNEV 250 260 270 280 290 300 NKIIKAIHFI GTFTVEFFID QQNQLYVNEI APRPHNSGHY SIEACEYSQF DTHIKAITGQ 310 320 330 340 350 360 QLPQQVTLLK PAIMMNLLGR DLDLLEDQFA EHPEWHVHIY GKNERKFNRK MGHLTVLTND 370 INETEQKLLK QFEGR

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE18870.1.
RefSeq	YP_301815.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q49WI9.
Genome annotation databases
GeneID	3616634.
GenomeReviews	Gene locus SSP1725 in contig AP008934_GR.
KEGG	ssp:SSP1725.
NMPDR	fig|342451.4.peg.1778.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q49WI9.
OMA	RKIGHVN.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP1725-MON.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
TIGRFAMs	TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_STAS1

Accession

Primary (citable) accession number: Q49WI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)