Q49WE6 (UGTP_STAS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Processive diacylglycerol glucosyltransferase
EC=2.4.1.-

Alternative name(s):
Beta-gentiobiosyldiacylglycerol synthase
DGlcDAG synthase
Diglucosyldiacylglycerol synthase
Glc2-DAG synthase
Monoglucosyldiacylglycerol synthase
Short name=MGlcDAG synthase
Triglucosyldiacylglycerol synthase
Short name=TGlcDAG synthase
UDP glucosyltransferase
UDP-glucose:1,2-diacylglycerol-3-beta-D-glucosyltransferase

Gene names

Name:	ugtP
Ordered Locus Names:	SSP1768

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is a processive glucosyltransferase involved in the synthesis of both bilayer- and non-bilayer-forming membrane glycolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of mono-, di- and triglucosyldiacylglycerol. Diglucosyldiacylglycerol (Glc2-DAG) is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA) By similarity. HAMAP MF_01280
Catalytic activity	UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol. HAMAP MF_01280 UDP-glucose + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol. HAMAP MF_01280 UDP-glucose + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol.
Pathway	Glycolipid metabolism; diglucosyl-diacylglycerol biosynthesis. HAMAP MF_01280
Sequence similarities	Belongs to the glycosyltransferase 28 family. UgtP subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolipid biosynthetic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro glucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	Processive diacylglycerol glucosyltransferase HAMAP MF_01280		PRO_0000308463

Sequences

	Sequence		Length	Mass (Da)
	Q49WE6 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 1E9545006B8A3A9E	FASTA	391	44,665
	10 20 30 40 50 60 MVTQNKKILI ITGSFGNGHL QVTQSVVNQF KEMNLDNLTV IEHDLFLEAH PILTSICKKW 70 80 90 100 110 120 YINSFKYFRN MYKAFYYSQP DQLDKCFYKY YGLNKLMNLL LKEKPDLILL TFPTPVMSVL 130 140 150 160 170 180 TEQFDMNIPI ATVMTDYRMQ KNWITPFSQR YYLATEELKD EFASIGIPKD KLKVTGIPIS 190 200 210 220 230 240 DKFETDIDKT AWLSQNHLDP DKPTILMSAG AFGVSKGFGQ MIQEILNRSP HAQVVMICGK 250 260 270 280 290 300 NKDLKRSLTS QFKDFNNVLI LGYTKHMNEW MASSQLMITK PGGITISEAL TRQIPMIFLD 310 320 330 340 350 360 PAPGQELENA VYFEEKGYGR IANTPEAAIE QVAALTNAPT KLASMSESMD ASRIPYSTYK 370 380 390 LCKDLLNLLN HSSHYEEVYG KVPLYAKLFV K

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008934 Genomic DNA. Translation: BAE18913.1.
RefSeq	YP_301858.1. NC_007350.1.
3D structure databases
ProteinModelPortal	Q49WE6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q49WE6.
Protein family/group databases
CAZy	GT28. Glycosyltransferase Family 28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000049003; EBSTAP00000047372; EBSTAG00000049000.
GeneID	3615395.
GenomeReviews	Gene locus SSP1768 in contig AP008934_GR.
KEGG	ssp:SSP1768.
NMPDR	fig|342451.4.peg.1618.
PATRIC	19625121. VBIStaSap90642_1764.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0707.
GeneTree	EBGT00050000024210.
HOGENOM	HBG535586.
OMA	EPAGHIV.
ProtClustDB	PRK13608.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP1768-MONOMER.
Family and domain databases
HAMAP	MF_01280. Diacylglyc_glucosyltr. [Tree]
InterPro	IPR009695. Diacylglyc_glucosyltr_N. IPR007235. Glyco_trans_28_C. IPR023589. Pro_diacylglycrl_glcsylTrfase. [Graphical view]
KO	K03429.
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF06925. MGDG_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UGTP_STAS1

Accession

Primary (citable) accession number: Q49WE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program