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Protein

Malate dehydrogenase

Gene

mdh

Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADUniRule annotation
Binding sitei85 – 851SubstrateUniRule annotation
Binding sitei91 – 911SubstrateUniRule annotation
Binding sitei98 – 981NADUniRule annotation
Binding sitei123 – 1231SubstrateUniRule annotation
Binding sitei154 – 1541SubstrateUniRule annotation
Active sitei178 – 1781Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156NADUniRule annotation
Nucleotide bindingi121 – 1233NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSSAP342451:GKFA-2091-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:SSP2027
OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Taxonomic identifieri342451 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000006371 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Malate dehydrogenasePRO_0000241968Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi342451.SSP2027.

Structurei

3D structure databases

ProteinModelPortaliQ49VN8.
SMRiQ49VN8. Positions 5-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiAGNVGEH.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q49VN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKKISIIG AGNTGATLAF IVAQHELADV VLIDRPDNEG QVKGKALDIF
60 70 80 90 100
ESSPVYGFDA KVTGSVNYAD TADSDIVVIT AGSPRKPGMS RDDLVQINEK
110 120 130 140 150
VMFDVTKEIV KYSPDCKIIV LTNPVDAMTY SVLKASGFPK ERVIGQSGVL
160 170 180 190 200
DTARYQSFIA EALNVSIKDI RGLVLGGHGD TMVPLVNSTN VNGVPLHQLL
210 220 230 240 250
NQTQIEQIVE RTRKGGAEIV ALLGNGSAYY APASAVFEMI EAILKDQHRL
260 270 280 290 300
LPSIALLEGE YGFSDICLGV PTVLSEKGIE NIVELALSDN EQAQLRISAD
310
SVEEVKQALK NQ
Length:312
Mass (Da):33,541
Last modified:September 13, 2005 - v1
Checksum:i7C014156766B7C32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA. Translation: BAE19172.1.
RefSeqiWP_002483968.1. NC_007350.1.
YP_302117.1. NC_007350.1.

Genome annotation databases

EnsemblBacteriaiBAE19172; BAE19172; SSP2027.
GeneIDi23779253.
KEGGissp:SSP2027.
PATRICi19625635. VBIStaSap90642_2020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA. Translation: BAE19172.1.
RefSeqiWP_002483968.1. NC_007350.1.
YP_302117.1. NC_007350.1.

3D structure databases

ProteinModelPortaliQ49VN8.
SMRiQ49VN8. Positions 5-305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi342451.SSP2027.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE19172; BAE19172; SSP2027.
GeneIDi23779253.
KEGGissp:SSP2027.
PATRICi19625635. VBIStaSap90642_2020.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiAGNVGEH.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciSSAP342451:GKFA-2091-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
    Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
    Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15305 / DSM 20229.

Entry informationi

Entry nameiMDH_STAS1
AccessioniPrimary (citable) accession number: Q49VN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: September 13, 2005
Last modified: May 27, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.