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Q49VN8 (MDH_STAS1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:SSP2027
OrganismStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]
Taxonomic identifier342451 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 312312Malate dehydrogenase HAMAP-Rule MF_00487
PRO_0000241968

Regions

Nucleotide binding10 – 156NAD By similarity
Nucleotide binding121 – 1233NAD By similarity

Sites

Active site1781Proton acceptor By similarity
Binding site341NAD By similarity
Binding site851Substrate By similarity
Binding site911Substrate By similarity
Binding site981NAD By similarity
Binding site1231Substrate By similarity
Binding site1541Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q49VN8 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 7C014156766B7C32

FASTA31233,541
        10         20         30         40         50         60 
MTKKKISIIG AGNTGATLAF IVAQHELADV VLIDRPDNEG QVKGKALDIF ESSPVYGFDA 

        70         80         90        100        110        120 
KVTGSVNYAD TADSDIVVIT AGSPRKPGMS RDDLVQINEK VMFDVTKEIV KYSPDCKIIV 

       130        140        150        160        170        180 
LTNPVDAMTY SVLKASGFPK ERVIGQSGVL DTARYQSFIA EALNVSIKDI RGLVLGGHGD 

       190        200        210        220        230        240 
TMVPLVNSTN VNGVPLHQLL NQTQIEQIVE RTRKGGAEIV ALLGNGSAYY APASAVFEMI 

       250        260        270        280        290        300 
EAILKDQHRL LPSIALLEGE YGFSDICLGV PTVLSEKGIE NIVELALSDN EQAQLRISAD 

       310 
SVEEVKQALK NQ 

« Hide

References

[1]"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection."
Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.
Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008934 Genomic DNA. Translation: BAE19172.1.
RefSeqYP_302117.1. NC_007350.1.

3D structure databases

ProteinModelPortalQ49VN8.
SMRQ49VN8. Positions 5-305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING342451.SSP2027.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE19172; BAE19172; SSP2027.
GeneID3615591.
KEGGssp:SSP2027.
PATRIC19625635. VBIStaSap90642_2020.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213794.
KOK00024.
OMAFVINVAN.
OrthoDBEOG6091FG.

Enzyme and pathway databases

BioCycSSAP342451:GKFA-2091-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01763. MalateDH_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_STAS1
AccessionPrimary (citable) accession number: Q49VN8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families