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Protein

Sensor histidine kinase GraS

Gene

graS

Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Member of the two-component regulatory system GraR/GraS involved in resistance against cationic antimicrobial peptides (CAMPs). GraS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to GraR (By similarity).By similarity

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAntibiotic resistance, Two-component regulatory system, Virulence
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase GraS (EC:2.7.13.3)
Alternative name(s):
Glycopeptide resistance-associated protein S
Gene namesi
Name:graS
Ordered Locus Names:SSP2061
OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Taxonomic identifieri342451 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006371 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Transmembranei43 – 63HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003479301 – 342Sensor histidine kinase GraSAdd BLAST342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei129Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi342451.SSP2061.

Structurei

3D structure databases

SMRiQ49VK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 332Histidine kinasePROSITE-ProRule annotationAdd BLAST207

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EWM. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000032012.
KOiK19077.
OrthoDBiPOG090Q032Y.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
PfamiView protein in Pfam
PF02518. HATPase_c. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiView protein in SMART
SM00387. HATPase_c. 1 hit.
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiView protein in PROSITE
PS50109. HIS_KIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q49VK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLKWFFIF LRSRIYWILW LLFLHFIFLG IAYLDYDISV YSILYIIILN
60 70 80 90 100
IGLSVLFLIF TYLKEVKFYK HLDENIEPEA LKHKSLADTP FQQEMVNYLY
110 120 130 140 150
GQITNQKALV THQKQQIQST EAALTDFVHD IKTPVTAMKL LIEKEQDLSR
160 170 180 190 200
KHALLYEWSR INDMLDRQLF LTRLEYQNKD MYFEHVPLKR LVIEEIQITR
210 220 230 240 250
YISQSKGIGY DLDFEDTLNV YTDTKWCRMM IRQVLSNAVK YSEESMIHIR
260 270 280 290 300
ALKESRHVVL EIKDEGKGIS EKDLPRIFDK GFTSTSNRNN TISSGLGLYL
310 320 330 340
VNHVKEKLSI CVRIHSELDK GTVVKFIFPN QNEIVAKLSQ NI
Length:342
Mass (Da):40,364
Last modified:September 2, 2008 - v2
Checksum:i59C36C286B8B12B2
GO

Sequence cautioni

The sequence BAE19206 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA. Translation: BAE19206.1. Different initiation.
RefSeqiWP_041784838.1. NC_007350.1.

Genome annotation databases

EnsemblBacteriaiBAE19206; BAE19206; SSP2061.
GeneIDi3615625.
KEGGissp:SSP2061.
PATRICi19625703. VBIStaSap90642_2054.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA. Translation: BAE19206.1. Different initiation.
RefSeqiWP_041784838.1. NC_007350.1.

3D structure databases

SMRiQ49VK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi342451.SSP2061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE19206; BAE19206; SSP2061.
GeneIDi3615625.
KEGGissp:SSP2061.
PATRICi19625703. VBIStaSap90642_2054.

Phylogenomic databases

eggNOGiENOG4105EWM. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000032012.
KOiK19077.
OrthoDBiPOG090Q032Y.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
PfamiView protein in Pfam
PF02518. HATPase_c. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiView protein in SMART
SM00387. HATPase_c. 1 hit.
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiView protein in PROSITE
PS50109. HIS_KIN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGRAS_STAS1
AccessioniPrimary (citable) accession number: Q49VK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: February 15, 2017
This is version 85 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.