Q49UX4 (SLE1_STAS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylmuramoyl-L-alanine amidase sle1
EC=3.5.1.28

Gene names

Name:	sle1
Synonyms:	aaa
Ordered Locus Names:	SSP2291

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus saprophyticus › Staphylococcus saprophyticus subsp. saprophyticus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptidoglycan hydrolase involved in the splitting of the septum during cell division By similarity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted By similarity. Cell surface By similarity.
Sequence similarities	Contains 3 LysM repeats. Contains 1 peptidase C51 domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell wall biogenesis/degradation Septation Virulence
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	barrier septum assembly Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 327	302	N-acetylmuramoyl-L-alanine amidase sle1		PRO_0000231630
Regions
	Repeat	29 – 71	43	LysM 1
	Repeat	88 – 130	43	LysM 2
	Repeat	152 – 194	43	LysM 3
	Domain	203 – 327	125	Peptidase C51

Sequences

	Sequence		Length	Mass (Da)
	Q49UX4 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 32B7759C57878F5A	FASTA	327	34,491
	10 20 30 40 50 60 MRKKIIATVI GTSALAAVTW TNADAATTYK VKSGDSLWSI ANKYNMSVAK LKSLNNLTSN 70 80 90 100 110 120 VIFPNQSLKV SGSTSSSTSS NTSTGSTYTV KSGDTLSGIA AKYGTTYQKI MSLNGLSNFN 130 140 150 160 170 180 IYPGQKLKVS GAASSSSSNT SGNTSSGSTT TYTVKSGDSL SAIAAKYGTT YQKIMSLNGL 190 200 210 220 230 240 TNFNIYPGQK LKVSGKASTG GSGSSSTGSA GYKTPVFNHS NLYDWGQCTW HVFNKRAQIG 250 260 270 280 290 300 KGISTYWWNA NNWDTAAAAD GYTIDRKATV GSILQSDMGY YGHVAFVESV NANGSITISE 310 320 MNYSASPGIV TYRTIPASQV SSYVYIH

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008934 Genomic DNA. Translation: BAE19436.1.
RefSeq	YP_302381.1. NC_007350.1.
3D structure databases
ProteinModelPortal	Q49UX4.
SMR	Q49UX4. Positions 214-327.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAE19436; BAE19436; SSP2291.
GeneID	3615679.
KEGG	ssp:SSP2291.
PATRIC	19626199. VBIStaSap90642_2282.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3942.
HOGENOM	HOG000279823.
OMA	LIASKYN.
ProtClustDB	CLSK884669.
Family and domain databases
InterPro	IPR007921. CHAP. IPR018392. Peptidoglycan-bd_lysin. IPR002482. Peptidoglycan-bd_Lysin_subgr. [Graphical view]
Pfam	PF05257. CHAP. 1 hit. PF01476. LysM. 3 hits. [Graphical view]
SMART	SM00257. LysM. 3 hits. [Graphical view]
PROSITE	PS50911. CHAP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SLE1_STAS1

Accession

Primary (citable) accession number: Q49UX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program