Reviewed, UniProtKB/Swiss-Prot Q49UL7 (CYSH_STAS1)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	SSP2409

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100648

Sequences

	Sequence		Length	Mass (Da)
	Q49UL7-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 9179A05F32ACDAB5	FASTA	242	27,742
	10 20 30 40 50 60 MSETKITYDD FNPHAFETLD ITDETKGARE VIRWAYDVYG DSIIYSCSFG AEGMILIDLI 70 80 90 100 110 120 SSVKKDAEIV FLDTNLHFQE TYDLIDRVKA RYPELNIKLK QPSLTLEEQA DQVAPALWKQ 130 140 150 160 170 180 DPNQCCYIRK IKPLEEVLSG ATAWVSGLRR EQSPSRQATN FINKDERFKS VKVCPLIHWT 190 200 210 220 230 240 WDDVWDYIKA HDLHYNELHD FNFPSIGCIP CTEAVSGNGD SRAGRWTNST KTECGLHTTN KP

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008934 Genomic DNA. Translation: BAE19554.1.
RefSeq	YP_302499.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3617313.
GenomeReviews	Gene locus SSP2409 in contig AP008934_GR.
KEGG	ssp:SSP2409.
NMPDR	fig|342451.4.peg.2409.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q49UL7.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP2409-MON.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. CysH. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_STAS1

Accession

Primary (citable) accession number: Q49UL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)