Q49UL7 (CYSH_STAS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	SSP2409

Organism

Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) [Complete proteome] [HAMAP]

Taxonomic identifier

342451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100648

Sequences

	Sequence		Length	Mass (Da)
	Q49UL7 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 9179A05F32ACDAB5	FASTA	242	27,742
	10 20 30 40 50 60 MSETKITYDD FNPHAFETLD ITDETKGARE VIRWAYDVYG DSIIYSCSFG AEGMILIDLI 70 80 90 100 110 120 SSVKKDAEIV FLDTNLHFQE TYDLIDRVKA RYPELNIKLK QPSLTLEEQA DQVAPALWKQ 130 140 150 160 170 180 DPNQCCYIRK IKPLEEVLSG ATAWVSGLRR EQSPSRQATN FINKDERFKS VKVCPLIHWT 190 200 210 220 230 240 WDDVWDYIKA HDLHYNELHD FNFPSIGCIP CTEAVSGNGD SRAGRWTNST KTECGLHTTN KP

References

[1]

"Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection." Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M., Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T. Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005) [PubMed: 16135568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15305 / DSM 20229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008934 Genomic DNA. Translation: BAE19554.1.
RefSeq	YP_302499.1. NC_007350.1.
3D structure databases
ProteinModelPortal	Q49UL7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q49UL7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000048051; EBSTAP00000046420; EBSTAG00000048048.
GeneID	3617313.
GenomeReviews	Gene locus SSP2409 in contig AP008934_GR.
KEGG	ssp:SSP2409.
NMPDR	fig|342451.4.peg.2409.
PATRIC	19626421. VBIStaSap90642_2394.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
GeneTree	EBGT00050000026343.
HOGENOM	HBG758022.
OMA	EDALENP.
PhylomeDB	Q49UL7.
ProtClustDB	CLSK313238.
Enzyme and pathway databases
BioCyc	SSAP342451:SSP2409-MONOMER.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMs	TIGR00434. CysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_STAS1

Accession

Primary (citable) accession number: Q49UL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program