Q49SQ1 (GPR33_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable G-protein coupled receptor 33

Gene names

Name:

GPR33

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Orphan receptor; could be a chemoattractant receptor.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in spleen, lung, heart, liver, kidney, pancreas, thymus, gonads and leukocytes. Ref.1
Polymorphism	In most individuals a nonsense mutation transforms the Arg-140 into a premature stop codon. Out of 1217 individuals, 1166 had a stop codon in both alleles, 50 were heterozygous and only one (in the Philippines) had Arg-140 in both alleles. GPR33 has undergone independent pseudogenization in human, chimpanzee, orangutan, siamang and rat. This selective inactivation may be due to its interaction with a putative pathogen that could use GPR33 as a receptor for cell invasion.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-formyl peptide receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Probable G-protein coupled receptor 33		PRO_0000069553
Regions
	Topological domain	1 – 30	30	Extracellular Potential
	Transmembrane	31 – 53	23	Helical; Name=1; Potential
	Topological domain	54 – 64	11	Cytoplasmic Potential
	Transmembrane	65 – 86	22	Helical; Name=2; Potential
	Topological domain	87 – 103	17	Extracellular Potential
	Transmembrane	104 – 124	21	Helical; Name=3; Potential
	Topological domain	125 – 143	19	Cytoplasmic Potential
	Transmembrane	144 – 165	22	Helical; Name=4; Potential
	Topological domain	166 – 209	44	Extracellular Potential
	Transmembrane	210 – 230	21	Helical; Name=5; Potential
	Topological domain	231 – 246	16	Cytoplasmic Potential
	Transmembrane	247 – 268	22	Helical; Name=6; Potential
	Topological domain	269 – 283	15	Extracellular Potential
	Transmembrane	284 – 303	20	Helical; Name=7; Potential
	Topological domain	304 – 333	30	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	12	1	N-linked (GlcNAc...) Potential
	Glycosylation	19	1	N-linked (GlcNAc...) Potential
	Glycosylation	272	1	N-linked (GlcNAc...) Potential
	Disulfide bond	101 ↔ 179		Potential

Sequences

	Sequence		Length	Mass (Da)
	Q49SQ1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 72AE74EEC8FE6CB8	FASTA	333	38,232
	10 20 30 40 50 60 MDLINSTDYL INASTLVRNS TQFLAPASKM IIALSLYISS IIGTITNGLY LWVLRFKMKQ 70 80 90 100 110 120 TVNTLLFFHL ILSYFISTMI LPFMATSQLQ DNHWNFGTAL CKVFNGTLSL GMFTSVFFLS 130 140 150 160 170 180 AIGLDRYLLT LHPVWSQQHR TPRWASSIVL GVWISAAALS IPYLIFRETH HDRKGKVTCQ 190 200 210 220 230 240 NNYAVSTNWE SKEMQASRQW IHVACFISRF LLGFLLPFFI IIFCYERVAS KVKERSLFKS 250 260 270 280 290 300 SKPFKVMMTA IISFFVCWMP YHIHQGLLLT TNQSLLLELT LILTVLTTSF NTIFSPTLYL 310 320 330 FVGENFKKVF KKSILALFES TFSEDSSVER TQT

References

[1]	"The rise and fall of the chemoattractant receptor GPR33." Roempler H., Schulz A., Pitra C., Coop G., Przeworski M., Paeaebo S., Schoeneberg T. J. Biol. Chem. 280:31068-31075(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], POLYMORPHISM, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY494000 Genomic DNA. Translation: AAR98754.1.
IPI	IPI00640769.
RefSeq	NP_001184113.2. NM_001197184.2.
UniGene	Hs.727479.
3D structure databases
ProteinModelPortal	Q49SQ1.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Polymorphism databases
DMDM	82592890.
Proteomic databases
PRIDE	Q49SQ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2856.
KEGG	hsa:2856.
Organism-specific databases
CTD	2856.
GeneCards	GC14M031953.
HGNC	HGNC:4489. GPR33.
MIM	610118. gene.
neXtProt	NX_Q49SQ1.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	HBG096748.
KO	K08382.
Gene expression databases
Genevestigator	Q49SQ1.
Family and domain databases
InterPro	IPR000826. Formyl_pep_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
PANTHER	PTHR24225. PTHR24225. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00526. FMETLEUPHER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	2856.
NextBio	11265.
SOURCE	Search...

Entry information

Entry name

GPR33_HUMAN

Accession

Primary (citable) accession number: Q49SQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.