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Q49SP7 (TPSCS_POGCB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Gamma-curcumene synthase
EC=4.2.3.94
Alternative name(s):
PatTpsA
OrganismPogostemon cablin (Patchouli) (Mentha cablin)
Taxonomic identifier28511 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Lamiales › Lamiaceae › Lamioideae › Pogostemoneae › Pogostemon

Protein attributes

Sequence length545 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sesquiterpene synthase involved in gamma-curcumene biosynthesis. Ref.1

Catalytic activity

(2E,6E)-farnesyl diphosphate = gamma-curcumene + diphosphate. Ref.1

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Subcellular location

Cytoplasm › cytosol By similarity.

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+ By similarity.

Sequence similarities

Belongs to the terpene synthase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionLyase
Gene Ontology (GO)
   Biological_processfarnesyl diphosphate metabolic process

Inferred from direct assay Ref.1. Source: UniProtKB

sesquiterpene biosynthetic process

Inferred from direct assay Ref.1. Source: UniProtKB

terpenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: InterPro

sesquiterpene synthase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 545545Gamma-curcumene synthase
PRO_0000419749

Regions

Motif299 – 3035DDXXD motif

Sites

Metal binding2991Magnesium 1 By similarity
Metal binding2991Magnesium 2 By similarity
Metal binding3031Magnesium 1 By similarity
Metal binding3031Magnesium 2 By similarity
Metal binding4421Magnesium 3 By similarity
Metal binding4501Magnesium 3 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q49SP7 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: E578CEC0DE21FB6E

FASTA54563,586
        10         20         30         40         50         60 
MAAFTANAVD MRPPVITIHP RSKDIFSQFS LDDKLQKQYA QGIEALKEEA RSMLMAAKSA 

        70         80         90        100        110        120 
KVMILIDTLE RLGLGYHFEK EIEEKLEAIY KKEDGDDYDL FTTALRFRLL RQHQRRVPCS 

       130        140        150        160        170        180 
VFDKFMNKEG KFEEEPLISD VEGLLSLYDA AYLQIHGEHI LQEALIFTTH HLTRIEPQLD 

       190        200        210        220        230        240 
DHSPLKLKLN RALEFPFYRE IPIIYAHFYI SVYERDDSRD EVLLKMAKLS YNFLQNLYKK 

       250        260        270        280        290        300 
ELSQLSRWWN KLELIPNLPY IRDSVAGAYL WAVALYFEPQ YSDVRMAIAK LIQIAAAVDD 

       310        320        330        340        350        360 
TYDNYATIRE AQLLTEALER LNVHEIDTLP DYMKIVYRFV MSWSEDFERD ATIKEQMLAT 

       370        380        390        400        410        420 
PYFKAEMKKL GRAYNQELKW VMERQLPSFE EYMKNSEITS GVYIMFTVIS PYLNSATQKN 

       430        440        450        460        470        480 
IDWLLSQPRL ASSTAIVMRC CNDLGSNQRE SKGGEVMTSL DCYMKQHGAS KQETISKFKL 

       490        500        510        520        530        540 
IIEDEWKNLN EEWAATTCLP KVMVEIFRNY ARIAGFCYKN NGDAYTSPKI VQQCFDALFV 


NPLRI

« Hide

References

[1]"The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated with a limited number of sesquiterpene synthases."
Deguerry F., Pastore L., Wu S., Clark A., Chappell J., Schalk M.
Arch. Biochem. Biophys. 454:123-136(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY508726 mRNA. Translation: AAS86319.1.

3D structure databases

ProteinModelPortalQ49SP7.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-14858.
UniPathwayUPA00213.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48239. Terp_cyc_toroid. 1 hit.
SSF48576. Terpenoid_synth. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTPSCS_POGCB
AccessionPrimary (citable) accession number: Q49SP7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: September 13, 2005
Last modified: May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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