Q49SP4 (TPGD1_POGCB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Germacrene D synthase 1 EC=4.2.3.75 Alternative name(s): PatTpsB15
Organism	Pogostemon cablin (Patchouli) (Mentha cablin)
Taxonomic identifier	28511 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Lamiales › Lamiaceae › Lamioideae › Pogostemoneae › Pogostemon

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sesquiterpene synthase involved in germacrene D biosynthesis. Produces also at least 13 additional sesquiterpene products, including germacrene C and (+)-germacrene A, beta-ylangene, (E)-beta-farnesene and (E,E)-alpha-farnesene. Ref.1
Catalytic activity	(2E,6E)-farnesyl diphosphate = (-)-germacrene D + diphosphate. Ref.1
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Secondary metabolite biosynthesis; terpenoid biosynthesis.
Subcellular location	Cytoplasm › cytosol By similarity.
Domain	The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+ By similarity.
Sequence similarities	Belongs to the terpene synthase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	farnesyl diphosphate metabolic process Inferred from direct assay Ref.1. Source: UniProtKB sesquiterpene biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB terpenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: InterPro sesquiterpene synthase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	Germacrene D synthase 1		PRO_0000419751
Regions
	Motif	298 – 302	5	DDXXD motif
Sites
	Metal binding	298	1	Magnesium 1 By similarity
	Metal binding	298	1	Magnesium 2 By similarity
	Metal binding	302	1	Magnesium 1 By similarity
	Metal binding	302	1	Magnesium 2 By similarity
	Metal binding	443	1	Magnesium 3 By similarity
	Metal binding	451	1	Magnesium 3 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49SP4 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: C3B5D57EE67FBCC2	FASTA	545	64,197
	10 20 30 40 50 60 MDLNEITSSS RPLANYHPNV WGDRFLLHEP EFTCQAGEKQ LVEELKEEVR RELKEASNDY 70 80 90 100 110 120 LRQLKMVDAI QRLGIEYLFE EEIDEALRNL LAKFENYCKD NHDMYATALS FRLLRQHGYK 130 140 150 160 170 180 VSCEVFDKFK DGEDGFKVEE VMAVLELFEA THMRIHGEDV LDQAFVFTRN YLQSIHATLS 190 200 210 220 230 240 NPIAKQVHNA LNGYSCRRGM PRIEARKYIP IYEEYGCHHK ALLKLAKLDF NLLQSMHKRE 250 260 270 280 290 300 LTQLYRWWKD LEMPTKLPYI RDRLVETYFW DMGFYFEPQY ALARNILVKV QCLVSIFDDT 310 320 330 340 350 360 FDAYGAFKEL QLFKDAIDRW SISCLDELPE YMQIIYKLVL DVFEEIESHM IKQGTSYRLD 370 380 390 400 410 420 YAREAIKIVI GGYFDEAKWR EEEYKPRMEE YMKVATKSAA YLTLIIVSFV GMKNDIATPQ 430 440 450 460 470 480 AFQWVLSEPQ IITASLALAR LSNDLVGIEF EKERKYIATA VELYEEEHKV SKEEAVLELR 490 500 510 520 530 540 HETESAWKEI NEALLEPTTF ATPILDRILN SARVLEVFYD KTDRYTHVDL ELQNIIAQLY IHPIP

References

[1]

"The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated with a limited number of sesquiterpene synthases." Deguerry F., Pastore L., Wu S., Clark A., Chappell J., Schalk M. Arch. Biochem. Biophys. 454:123-136(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY508729 mRNA. Translation: AAS86322.1.
3D structure databases
ProteinModelPortal	Q49SP4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14835.
UniPathway	UPA00213.
Family and domain databases
Gene3D	1.10.600.10. 1 hit. 1.50.30.10. 1 hit.
InterPro	IPR001906. Terpene_synth_N. IPR005630. Terpene_synthase_metal-bd. IPR008930. Terpenoid_cyclase/PrenylTrfase. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF01397. Terpene_synth. 1 hit. PF03936. Terpene_synth_C. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 1 hit. SSF48576. Terpenoid_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPGD1_POGCB

Accession

Primary (citable) accession number: Q49SP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2012
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program