G-protein-coupled receptor kinase 7A precursor

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Q49HM9 (GRK7A_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 65. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
G-protein-coupled receptor kinase 7A
EC=2.7.11.14
EC=2.7.11.16

Alternative name(s):
G protein-coupled receptor kinase 7-1

Gene names

Name:	grk7a
Synonyms:	grk7-1
ORF Names:	dkeyp-13a3.1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO). Ref.1
Catalytic activity	ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate. Ref.2 ATP + [rhodopsin] = ADP + [rhodopsin] phosphate. Ref.2
Subcellular location	Membrane; Lipid-anchor By similarity.
Post-translational modification	Phosphorylation at Ser-33 is regulated by light and activated by cAMP By similarity.
Disruption phenotype	Impaired cone response recovery and delayed dark adaptation. Ref.1
Miscellaneous	Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.
Biophysicochemical properties	Kinetic parameters: K_M=4.4 µM for rhodopsin Ref.2 V_max=773 nmol/min/mg enzyme

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phototransduction, visible light Inferred from mutant phenotype Ref.1. Source: ZFIN termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro visual perception Inferred from mutant phenotype PubMed 21299656. Source: ZFIN
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: EC photoreceptor activity Inferred from mutant phenotype Ref.1. Source: ZFIN rhodopsin kinase activity Inferred from direct assay Ref.2. Source: ZFIN
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 546

546

G-protein-coupled receptor kinase 7A

PRO_0000412812

Propeptide

547 – 549

Removed in mature form By similarity

PRO_0000412813

Regions

Domain

53 – 171

119

RGS

Domain

186 – 449

264

Protein kinase

Domain

450 – 515

AGC-kinase C-terminal

Nucleotide binding

192 – 200

ATP By similarity

Sites

Active site

311

Proton acceptor By similarity

Binding site

215

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

546

Cysteine methyl ester Potential

Lipidation

546

S-geranylgeranyl cysteine Potential

Experimental info

Sequence conflict

T → I in AAX69081. Ref.1

Sequence conflict

385

F → I in AAX69081. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q49HM9 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 3F76C291F8BC9716
Show »

FASTA

549

62,232

        10         20         30         40         50         60 
MCDMGGLDNL VANTAYLKAQ GGDDKEMKKR RRSLSLPKPE QCASLRTSLD KDFESLCEKQ 

        70         80         90        100        110        120 
PIGKKLFRQY LSQGGPECTT AAEFLDDLNE WELSESAARD KARTNIINKF CKEGSKSSLT 

       130        140        150        160        170        180 
FLTGDVATKC KAVSDKDFEE VMGQVKTATK EFLKGKPFTE YQASPFFDKF LQWKEYEKQP 

       190        200        210        220        230        240 
ISEKYFYEFR TLGKGGFGEV CAVQVKNTGQ MYACKKLCKK RLKKKHGEKM ALLEKKILER 

       250        260        270        280        290        300 
VNSLFIVSLA YAYDTKTHLC LVMSLMNGGD LKYHIYNIGE KGIEMDRIIY YTAQIATGIL 

       310        320        330        340        350        360 
HLHDMDIVYR DMKPENVLLD SQGQCRLSDL GLAVEIAVGK TISQKAGTGA YMAPEILNET 

       370        380        390        400        410        420 
PYRTSVDWWA LGCSIYEMVA GYTPFKGPDA KKEKVEKEEV QRRILNEEPK FEHKNFDAAT 

       430        440        450        460        470        480 
IDIIKQFLKK KIDERLGCKN DDPRKHEWFK SINFARLEAG LIDPPWVPKP NVVYAKDTGD 

       490        500        510        520        530        540 
IAEFSEIKGI EFDAKDDKFF KEFSTGAVSI AWQQEMIDTG LFDELSDPNR KESSGGSDDD 


KKSGTCTLL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Knockdown of cone-specific kinase GRK7 in larval zebrafish leads to impaired cone response recovery and delayed dark adaptation." Rinner O., Makhankov Y.V., Biehlmaier O., Neuhauss S.C. Neuron 47:231-242(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE.
[2]	"GRK1 and GRK7: unique cellular distribution and widely different activities of opsin phosphorylation in the zebrafish rods and cones." Wada Y., Sugiyama J., Okano T., Fukada Y. J. Neurochem. 98:824-837(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[3]	The Danio rerio Sequencing Project at the Sanger Institute Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[4]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY900004 mRNA. Translation: AAX69081.1. AB212995 mRNA. Translation: BAE92858.1. CR377211 Genomic DNA. Translation: CAQ13370.1. BC163587 mRNA. Translation: AAI63587.1.
IPI	IPI00611992.
RefSeq	NP_001027011.2. NM_001031841.2.
UniGene	Dr.82672.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000091060.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000100287; ENSDARP00000091060; ENSDARG00000020602.
GeneID	566120.
KEGG	dre:566120.
Organism-specific databases
CTD	566120.
ZFIN	ZDB-GENE-050824-1. grk7a.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00550000074318.
HOGENOM	HOG000006742.
HOVERGEN	HBG004532.
KO	K00909.
OMA	ESKSHLC.
OrthoDB	EOG4TTGJ1.
Enzyme and pathway databases
BRENDA	2.7.11.14. 96826.
Gene expression databases
Bgee	Q49HM9.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20888040.

Entry information

Entry name

GRK7A_DANRE

Accession

Primary (citable) accession number: Q49HM9
Secondary accession number(s): Q1XHL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	September 21, 2011
Last modified:	April 3, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents