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Protein

Rhodopsin kinase 1

Gene

grk7a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).1 Publication

Catalytic activityi

ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.1 Publication

Kineticsi

  1. KM=4.4 µM for rhodopsin1 Publication
  1. Vmax=773 nmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei215 – 2151ATPPROSITE-ProRule annotation
Active sitei311 – 3111Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi192 – 2009ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • G-protein coupled receptor kinase activity Source: InterPro
  • photoreceptor activity Source: ZFIN
  • rhodopsin kinase activity Source: ZFIN

GO - Biological processi

  • phototransduction, visible light Source: ZFIN
  • visual perception Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.14. 928.
ReactomeiR-DRE-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhodopsin kinase 1 (EC:2.7.11.141 Publication)
Alternative name(s):
G protein-coupled receptor kinase 7-1
G-protein-coupled receptor kinase 7A
Gene namesi
Name:grk7a
Synonyms:grk7-1
ORF Names:dkeyp-13a3.1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 2

Organism-specific databases

ZFINiZDB-GENE-050824-1. grk7a.

Subcellular locationi

  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired cone response recovery and delayed dark adaptation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 546546Rhodopsin kinase 1PRO_0000412812Add
BLAST
Propeptidei547 – 5493Removed in mature formBy similarityPRO_0000412813

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331PhosphoserineBy similarity
Modified residuei546 – 5461Cysteine methyl esterSequence analysis
Lipidationi546 – 5461S-geranylgeranyl cysteineSequence analysis

Post-translational modificationi

Phosphorylation at Ser-33 is regulated by light and activated by cAMP.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ49HM9.

Expressioni

Gene expression databases

BgeeiENSDARG00000020602.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000091060.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 171119RGSPROSITE-ProRule annotationAdd
BLAST
Domaini186 – 449264Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini450 – 51566AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0986. Eukaryota.
ENOG410YRQZ. LUCA.
GeneTreeiENSGT00770000120493.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiQ49HM9.
KOiK00909.
OMAiESKSHLC.
OrthoDBiEOG091G062G.
TreeFamiTF313940.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q49HM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCDMGGLDNL VANTAYLKAQ GGDDKEMKKR RRSLSLPKPE QCASLRTSLD
60 70 80 90 100
KDFESLCEKQ PIGKKLFRQY LSQGGPECTT AAEFLDDLNE WELSESAARD
110 120 130 140 150
KARTNIINKF CKEGSKSSLT FLTGDVATKC KAVSDKDFEE VMGQVKTATK
160 170 180 190 200
EFLKGKPFTE YQASPFFDKF LQWKEYEKQP ISEKYFYEFR TLGKGGFGEV
210 220 230 240 250
CAVQVKNTGQ MYACKKLCKK RLKKKHGEKM ALLEKKILER VNSLFIVSLA
260 270 280 290 300
YAYDTKTHLC LVMSLMNGGD LKYHIYNIGE KGIEMDRIIY YTAQIATGIL
310 320 330 340 350
HLHDMDIVYR DMKPENVLLD SQGQCRLSDL GLAVEIAVGK TISQKAGTGA
360 370 380 390 400
YMAPEILNET PYRTSVDWWA LGCSIYEMVA GYTPFKGPDA KKEKVEKEEV
410 420 430 440 450
QRRILNEEPK FEHKNFDAAT IDIIKQFLKK KIDERLGCKN DDPRKHEWFK
460 470 480 490 500
SINFARLEAG LIDPPWVPKP NVVYAKDTGD IAEFSEIKGI EFDAKDDKFF
510 520 530 540
KEFSTGAVSI AWQQEMIDTG LFDELSDPNR KESSGGSDDD KKSGTCTLL
Length:549
Mass (Da):62,232
Last modified:September 21, 2011 - v2
Checksum:i3F76C291F8BC9716
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti80 – 801T → I in AAX69081 (PubMed:16039565).Curated
Sequence conflicti385 – 3851F → I in AAX69081 (PubMed:16039565).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY900004 mRNA. Translation: AAX69081.1.
AB212995 mRNA. Translation: BAE92858.1.
CR377211 Genomic DNA. Translation: CAQ13370.1.
BC163587 mRNA. Translation: AAI63587.1.
RefSeqiNP_001027011.2. NM_001031841.3.
UniGeneiDr.82672.

Genome annotation databases

EnsembliENSDART00000100287; ENSDARP00000091060; ENSDARG00000020602.
GeneIDi566120.
KEGGidre:566120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY900004 mRNA. Translation: AAX69081.1.
AB212995 mRNA. Translation: BAE92858.1.
CR377211 Genomic DNA. Translation: CAQ13370.1.
BC163587 mRNA. Translation: AAI63587.1.
RefSeqiNP_001027011.2. NM_001031841.3.
UniGeneiDr.82672.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000091060.

Proteomic databases

PaxDbiQ49HM9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000100287; ENSDARP00000091060; ENSDARG00000020602.
GeneIDi566120.
KEGGidre:566120.

Organism-specific databases

CTDi566120.
ZFINiZDB-GENE-050824-1. grk7a.

Phylogenomic databases

eggNOGiKOG0986. Eukaryota.
ENOG410YRQZ. LUCA.
GeneTreeiENSGT00770000120493.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiQ49HM9.
KOiK00909.
OMAiESKSHLC.
OrthoDBiEOG091G062G.
TreeFamiTF313940.

Enzyme and pathway databases

BRENDAi2.7.11.14. 928.
ReactomeiR-DRE-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

PROiQ49HM9.

Gene expression databases

BgeeiENSDARG00000020602.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRK7A_DANRE
AccessioniPrimary (citable) accession number: Q49HM9
Secondary accession number(s): Q1XHL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: September 7, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.