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Q49HM9

- GRK7A_DANRE

UniProt

Q49HM9 - GRK7A_DANRE

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Protein

G-protein-coupled receptor kinase 7A

Gene

grk7a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).1 Publication

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.1 Publication
ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.1 Publication

Kineticsi

  1. KM=4.4 µM for rhodopsin1 Publication

Vmax=773 nmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei215 – 2151ATPPROSITE-ProRule annotation
Active sitei311 – 3111Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi192 – 2009ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. G-protein coupled receptor kinase activity Source: UniProtKB-EC
  3. photoreceptor activity Source: ZFIN
  4. rhodopsin kinase activity Source: ZFIN

GO - Biological processi

  1. phototransduction, visible light Source: ZFIN
  2. termination of G-protein coupled receptor signaling pathway Source: InterPro
  3. visual perception Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.14. 96826.

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein-coupled receptor kinase 7A (EC:2.7.11.141 Publication, EC:2.7.11.16)
Alternative name(s):
G protein-coupled receptor kinase 7-1
Gene namesi
Name:grk7a
Synonyms:grk7-1
ORF Names:dkeyp-13a3.1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 2

Organism-specific databases

ZFINiZDB-GENE-050824-1. grk7a.

Subcellular locationi

Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

  1. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired cone response recovery and delayed dark adaptation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 546546G-protein-coupled receptor kinase 7APRO_0000412812Add
BLAST
Propeptidei547 – 5493Removed in mature formBy similarityPRO_0000412813

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331PhosphoserineBy similarity
Modified residuei546 – 5461Cysteine methyl esterSequence Analysis
Lipidationi546 – 5461S-geranylgeranyl cysteineSequence Analysis

Post-translational modificationi

Phosphorylation at Ser-33 is regulated by light and activated by cAMP.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Expressioni

Gene expression databases

BgeeiQ49HM9.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000091060.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 171119RGSPROSITE-ProRule annotationAdd
BLAST
Domaini186 – 449264Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini450 – 51566AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120493.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiQ49HM9.
KOiK00909.
OMAiAYAFESK.
OrthoDBiEOG7V1FQK.
TreeFamiTF313940.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q49HM9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCDMGGLDNL VANTAYLKAQ GGDDKEMKKR RRSLSLPKPE QCASLRTSLD
60 70 80 90 100
KDFESLCEKQ PIGKKLFRQY LSQGGPECTT AAEFLDDLNE WELSESAARD
110 120 130 140 150
KARTNIINKF CKEGSKSSLT FLTGDVATKC KAVSDKDFEE VMGQVKTATK
160 170 180 190 200
EFLKGKPFTE YQASPFFDKF LQWKEYEKQP ISEKYFYEFR TLGKGGFGEV
210 220 230 240 250
CAVQVKNTGQ MYACKKLCKK RLKKKHGEKM ALLEKKILER VNSLFIVSLA
260 270 280 290 300
YAYDTKTHLC LVMSLMNGGD LKYHIYNIGE KGIEMDRIIY YTAQIATGIL
310 320 330 340 350
HLHDMDIVYR DMKPENVLLD SQGQCRLSDL GLAVEIAVGK TISQKAGTGA
360 370 380 390 400
YMAPEILNET PYRTSVDWWA LGCSIYEMVA GYTPFKGPDA KKEKVEKEEV
410 420 430 440 450
QRRILNEEPK FEHKNFDAAT IDIIKQFLKK KIDERLGCKN DDPRKHEWFK
460 470 480 490 500
SINFARLEAG LIDPPWVPKP NVVYAKDTGD IAEFSEIKGI EFDAKDDKFF
510 520 530 540
KEFSTGAVSI AWQQEMIDTG LFDELSDPNR KESSGGSDDD KKSGTCTLL
Length:549
Mass (Da):62,232
Last modified:September 21, 2011 - v2
Checksum:i3F76C291F8BC9716
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti80 – 801T → I in AAX69081. (PubMed:16039565)Curated
Sequence conflicti385 – 3851F → I in AAX69081. (PubMed:16039565)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY900004 mRNA. Translation: AAX69081.1.
AB212995 mRNA. Translation: BAE92858.1.
CR377211 Genomic DNA. Translation: CAQ13370.1.
BC163587 mRNA. Translation: AAI63587.1.
RefSeqiNP_001027011.2. NM_001031841.3.
UniGeneiDr.82672.

Genome annotation databases

EnsembliENSDART00000100287; ENSDARP00000091060; ENSDARG00000020602.
GeneIDi566120.
KEGGidre:566120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY900004 mRNA. Translation: AAX69081.1 .
AB212995 mRNA. Translation: BAE92858.1 .
CR377211 Genomic DNA. Translation: CAQ13370.1 .
BC163587 mRNA. Translation: AAI63587.1 .
RefSeqi NP_001027011.2. NM_001031841.3.
UniGenei Dr.82672.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7955.ENSDARP00000091060.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000100287 ; ENSDARP00000091060 ; ENSDARG00000020602 .
GeneIDi 566120.
KEGGi dre:566120.

Organism-specific databases

CTDi 566120.
ZFINi ZDB-GENE-050824-1. grk7a.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00770000120493.
HOGENOMi HOG000006742.
HOVERGENi HBG004532.
InParanoidi Q49HM9.
KOi K00909.
OMAi AYAFESK.
OrthoDBi EOG7V1FQK.
TreeFami TF313940.

Enzyme and pathway databases

BRENDAi 2.7.11.14. 96826.

Miscellaneous databases

NextBioi 20888040.
PROi Q49HM9.

Gene expression databases

Bgeei Q49HM9.

Family and domain databases

InterProi IPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR00717. GPCRKINASE.
SMARTi SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Knockdown of cone-specific kinase GRK7 in larval zebrafish leads to impaired cone response recovery and delayed dark adaptation."
    Rinner O., Makhankov Y.V., Biehlmaier O., Neuhauss S.C.
    Neuron 47:231-242(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE.
  2. "GRK1 and GRK7: unique cellular distribution and widely different activities of opsin phosphorylation in the zebrafish rods and cones."
    Wada Y., Sugiyama J., Okano T., Fukada Y.
    J. Neurochem. 98:824-837(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  3. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  4. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiGRK7A_DANRE
AccessioniPrimary (citable) accession number: Q49HM9
Secondary accession number(s): Q1XHL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: November 26, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3