Reviewed, UniProtKB/Swiss-Prot Q49GP5 (P4K2B_DANRE)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 4-kinase type 2-beta
    EC=2.7.1.67
Alternative name(s):
    Phosphatidylinositol 4-kinase type II-beta

Gene names

Name:	pi4k2b
ORF Names:	zgc:158305

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3) By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Subcellular location	Cytoplasm By similarity. Membrane By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Phosphatidylinositol 4-kinase type 2-beta		PRO_0000285168
Regions
	Domain	150 – 456	307	PI3K/PI4K
	Compositional bias	102 – 105	4	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	Q49GP5-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 1E615C79533140B4	FASTA	501	56,199
	10 20 30 40 50 60 MMAECDPTDG EPGNGSGDST PETNFLSSEV PAVLFEKTRS APSLGLSLNP GAAVRISDST 70 80 90 100 110 120 ESVLTELEAD GSGEEALLLP GPAGSLSPRA GKEKRTRRNM LSSSSDNLAS PGNSSGEFNH 130 140 150 160 170 180 FPEDPEFGEI IQRAEQAIEN GVFPERISQG SSGSYFVKDP KGKIIGVFKP KSEEPYGHLN 190 200 210 220 230 240 PKWTKYFHKV CCPCCFGRGC LIPNQGYLSE AAASLVDQKL GLWIVPKTKV VHLASETFHY 250 260 270 280 290 300 NAIDRAKSRG KKYALEKVPK VGRRFHRVGL PPKVGSFQLF VEGYHEADFW LRKFEAEPLP 310 320 330 340 350 360 ENMRKQLQSQ FERLVVLDYV IRNTDRGNDN WLIKYEKPGD GELTEKESEW TDPKDSAIKI 370 380 390 400 410 420 AAIDNGLAFP FKHPDEWRAY PFHWAWLPQA KVAFSQETRD LVLSRISDMN FVQDLCEDLY 430 440 450 460 470 480 EMFRTDKGFD KTMFEKQMSV MRGQILNLTQ ALKDGKSPIQ LVQMPRVVVE RSRSGGQGRV 490 500 VQLGNAFTQT FHCKRPFFTS W

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phosphatidylinositol 4-kinases of Danio rerio." Ma H., Balla T. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY929291 mRNA. Translation: AAY16566.1. BC129195 mRNA. Translation: AAI29196.1.
IPI	IPI00510481.
RefSeq	NP_001038950.1.
UniGene	Dr.48536
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDART00000033018; ENSDARP00000038731; ENSDARG00000013881; Danio rerio. [Genome view]
GeneID	564750.
KEGG	dre:564750.
Organism-specific databases
ZFIN	ZDB-GENE-070112-990. zgc:158305.
Phylogenomic databases
HOVERGEN	Q49GP5.
Enzyme and pathway databases
BRENDA	2.7.1.67. 96826.
Gene expression databases
ArrayExpress	Q49GP5.
Bgee	Q49GP5.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. [Graphical view]
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. False negative. PS50290. PI3_4_KINASE_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P4K2B_DANRE

Accession

Primary (citable) accession number: Q49GP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	September 13, 2005
Last modified:	October 13, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project