Reviewed, UniProtKB/Swiss-Prot Q49GP3 (PI4KB_DANRE)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 4-kinase beta
      Short name=PtdIns 4-kinase beta
      Short name=PI4K-beta
      Short name=PI4Kbeta
    EC=2.7.1.67

Gene names

Name:	pi4kb
Synonyms:	pik4cb

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	835 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP) By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Subcellular location	Endomembrane system By similarity. Mitochondrion outer membrane; Peripheral membrane protein By similarity. Rough endoplasmic reticulum membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. Contains 1 PI3K/PI4K domain.
Sequence caution	The sequence ACH92118.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phosphoinositide phosphorylation Inferred from electronic annotation. Source: InterPro phosphoinositide-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular component	endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 835	835	Phosphatidylinositol 4-kinase beta		PRO_0000365169
Regions
	Domain	578 – 784	207	PI3K/PI4K
	Compositional bias	19 – 56	38	Ser-rich
	Compositional bias	277 – 286	10	Poly-Pro
Experimental info
	Sequence conflict	100	1	S → L in AAY16568. Ref.1
	Sequence conflict	370	1	R → G in AAI24585. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q49GP3-1 [UniParc]. Last modified March 3, 2009. Version 2. Checksum: DE044DB4C925B795	FASTA	835	94,059
	10 20 30 40 50 60 MGDTELELSP THLEELQKSP STSTTSSLSL PSSPSSGPHP LTSSSPSTSE GLPTSSPPLD 70 80 90 100 110 120 VISEGLGELS LVIDTEVAKK ACQEVLQKVK FLKGDGEVSS ASSEPILANG TAHPEANDGG 130 140 150 160 170 180 QPPKISEEEV EPIKSVRRRQ KNNSSKQSWL LRLFESKLFD ISMAISYLYN SKEPGVQAYI 190 200 210 220 230 240 GNRLFSFRNE DVDFYLPQLL NMYIHMDEDV GDAIKPYVVY RCRQSINFSL QCAWLLGAYS 250 260 270 280 290 300 SDMHISTQRH SRGTKLRKLI LSDELKPSSQ RIRREVPQPP PPYPPPLHHG PGMSEHSLSP 310 320 330 340 350 360 SKRTHQRSKS DATVSISLSS NLKRTASNPK VETSQDEPVR LTPQREFIKS LMGIGKRLAT 370 380 390 400 410 420 LPTKEQKTQR LISELSLLNH KLPARVWLPT AAFDHHVVRV PHTQAVVLNS KDKAPYLIYV 430 440 450 460 470 480 EVLECENFET SSVPVRIPET QIRSTRSVEN LPDCGITPDQ RASSFSTVPN YDNDDEAWSV 490 500 510 520 530 540 DDIGELQVEL PEIHTNSCDN ISQFSVDSIT SQESKEPIFI AAGDIRRRLS EQLAHTPTTF 550 560 570 580 590 600 RKDPEDPSAV ALKEPWQEKV RRIREGSPYG HLPNWRLLSV IVKCGDDLRQ ELLAYQVLKQ 610 620 630 640 650 660 LQIIWEQERV PLWIKPYKIL VISSDSGMIE PVVNAVSIHQ VKKQSQLLLL DYFRQEHGNF 670 680 690 700 710 720 NTEEFLTAQR NFVQSCAGYC LICYLLQVKD RHNGNILLDS EGHIIHIDFG FILSSSPRNL 730 740 750 760 770 780 GFETSAFKLT SEFVDVMGGL DGDMFNYYKM LMLQGLIAAR KHMEKVIQIV EIMQQGSQLP 790 800 810 820 830 CFHGSSTIRN LKERFHMNLT EEQLQVLVEQ MVDGSMRSIT TKLYDGFQYL TNGIM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phosphatidylinositol 4-kinases of Danio rerio." Ma H., Balla T. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Proteomic analysis of NCS-1 interacting proteins reveals novel signaling pathways required for inner ear development in zebrafish." Petko J., Kabbani N., Woll M., Decotiis D., Frey C., Hickey K., Craig M., Canfield V., Levenson R. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY929293 mRNA. Translation: AAY16568.1. FJ032032 mRNA. Translation: ACH92118.1. Sequence problems. BC124584 mRNA. Translation: AAI24585.1.
IPI	IPI00803559.
RefSeq	NP_001030149.2.
UniGene	Dr.48538
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDART00000058666; ENSDARP00000058665; ENSDARG00000040111; Danio rerio. [Genome view] ENSDART00000112165; ENSDARP00000099330; ENSDARG00000040111; Danio rerio. [Genome view]
GeneID	563201.
KEGG	dre:563201.
Organism-specific databases
CTD	563201.
ZFIN	ZDB-GENE-061013-96. pi4kb.
Phylogenomic databases
HOGENOM	Q49GP3.
HOVERGEN	Q49GP3.
OMA	DMVVEPA.
Gene expression databases
ArrayExpress	Q49GP3.
Bgee	Q49GP3.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR015433. PI_Kinase. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI4KB_DANRE

Accession

Primary (citable) accession number: Q49GP3
Secondary accession number(s): B5TXD8, Q08BS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	November 3, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project