Reviewed, UniProtKB/Swiss-Prot Q49AH0 (CDNF_HUMAN)

Names and origin

Protein names

Recommended name:
Cerebral dopamine neurotrophic factor

Alternative name(s):
Conserved dopamine neurotrophic factor
ARMET-like protein 1

Gene names

Name:	CDNF
Synonyms:	ARMETL1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra By similarity.
Subcellular location	Secreted Ref.4 Ref.5.
Tissue specificity	Widely expressed in neuronal and non-neuronal tissues. In the brain, highest levels in the optic nerve and corpus callosum. Ref.4
Induction	Not induced by endoplasmic reticulum (ER) stress. Ref.5
Sequence similarities	Belongs to the ARMET family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Growth factor
PTM	Disulfide bond
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q49AH0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q49AH0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Potential

Chain

25 – 187

163

Cerebral dopamine neurotrophic factor

PRO_0000281137

Amino acid modifications

Disulfide bond

37 ↔ 124

Ref.6

Disulfide bond

40 ↔ 113

Ref.6

Disulfide bond

71 ↔ 82

Ref.6

Natural variations

Alternative sequence

1 – 102

102

Missing in isoform 2.

VSP_023986

Secondary structure

1

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187

Helix Strand Turn

Details...

Helix

38 – 53

16

Helix

60 – 71

12

Helix

78 – 84

7

Helix

93 – 103

11

Turn

104 – 106

3

Helix

109 – 117

9

Helix

121 – 124

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: 87460391592A10CA	FASTA	187	20,964
	10 20 30 40 50 60 MWCASPVAVV AFCAGLLVSH PVLTQGQEAG GRPGADCEVC KEFLNRFYKS LIDRGVNFSL 70 80 90 100 110 120 DTIEKELISF CLDTKGKENR LCYYLGATKD AATKILSEVT RPMSVHMPAM KICEKLKKLD 130 140 150 160 170 180 SQICELKYEK TLDLASVDLR KMRVAELKQI LHSWGEECRA CAEKTDYVNL IQELAPKYAA THPKTEL
	Isoform 2. Checksum: 6DF6CCDF820C8838	FASTA	85	9,806
	10 20 30 40 50 60 MSVHMPAMKI CEKLKKLDSQ ICELKYEKTL DLASVDLRKM RVAELKQILH SWGEECRACA 70 80 EKTDYVNLIQ ELAPKYAATH PKTEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Spleen.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Testis.
[4]	"Novel neurotrophic factor CDNF protects and rescues midbrain dopamine neurons in vivo." Lindholm P., Voutilainen M.H., Lauren J., Peraenen J., Leppaenen V.-M., Andressoo J.-O., Lindahl M., Janhunen S., Kalkkinen N., Timmusk T., Tuominen R.K., Saarma M. Nature 448:73-77(2007) [PubMed: 17611540] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"Armet, a UPR-upregulated protein, inhibits cell proliferation and ER stress-induced cell death." Apostolou A., Shen Y., Liang Y., Luo J., Fang S. Exp. Cell Res. 314:2454-2467(2008) [PubMed: 18561914] [Abstract] Cited for: SUBCELLULAR LOCATION, INDUCTION.
[6]	"The structure of the conserved neurotrophic factors MANF and CDNF explains why they are bifunctional." Parkash V., Lindholm P., Peranen J., Kalkkinen N., Oksanen E., Saarma M., Leppanen V.-M., Goldman A. Protein Eng. Des. Sel. 22:233-241(2009) [PubMed: 19258449] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 32-133, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK301259 mRNA. Translation: BAG62825.1.
CH471072 Genomic DNA. Translation: EAW86264.1.
BC037872 mRNA. Translation: AAH37872.1.
BC133042 mRNA. Translation: AAI33043.1.
BC133044 mRNA. Translation: AAI33045.1.

IPI

IPI00646351.
IPI00647956.

RefSeq

NP_001025125.2.

UniGene

Hs.559067

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2W50	X-ray	1.60	A/B	32-133	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q49AH0.

PTM databases

PhosphoSite

Q49AH0.

Proteomic databases

PRIDE

Q49AH0.

Genome annotation databases

Ensembl

ENST00000465530; ENSP00000419395; ENSG00000185267; Homo sapiens. [Genome view]

GeneID

441549.

KEGG

hsa:441549.

UCSC

uc001inb.1. human.
uc001inc.1. human.

Organism-specific databases

CTD

441549.

GeneCards

GC10M014866.

HGNC

HGNC:24913. CDNF.

MIM

611233. gene.

PharmGKB

PA134870440.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG08973.

InParanoid

Q49AH0.

OMA

GADCEVC.

OrthoDB

EOG9CZF3T.

PhylomeDB

Q49AH0.

Gene expression databases

ArrayExpress

Q49AH0.

Bgee

Q49AH0.

CleanEx

HS_ARMETL1.

Genevestigator

Q49AH0.

Family and domain databases

InterPro

IPR019345. Armet_prot.
[Graphical view]

Pfam

PF10208. Armet. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

110303.

SOURCE

Search...

Entry information

Entry name

CDNF_HUMAN

Accession

Primary (citable) accession number: Q49AH0
Secondary accession number(s): A2RUU0, B4DVW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	January 19, 2010
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.