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Protein

Putative oxidoreductase GLYR1

Gene

GLYR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have oxidoreductase activity. Regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 and activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6. Recruited on chromatin, recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei362NADBy similarity1
Binding sitei505NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi168 – 180A.T hookAdd BLAST13
Nucleotide bindingi271 – 285NADBy similarityAdd BLAST15

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

DNA-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Putative oxidoreductase GLYR1 (EC:1.-.-.-)
Alternative name(s):
3-hydroxyisobutyrate dehydrogenase-like protein
Cytokine-like nuclear factor N-PAC
Glyoxylate reductase 1 homolog
Nuclear protein NP60
Nuclear protein of 60 kDa
Gene namesi
Name:GLYR1
Synonyms:HIBDL, NP60
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:24434. GLYR1.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165450093.

Polymorphism and mutation databases

BioMutaiGLYR1.
DMDMi269849681.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003121211 – 553Putative oxidoreductase GLYR1Add BLAST553

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei130PhosphoserineCombined sources1
Cross-linki135Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei167PhosphoserineCombined sources1
Cross-linki227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki237Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki269Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei540PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ49A26.
MaxQBiQ49A26.
PaxDbiQ49A26.
PeptideAtlasiQ49A26.
PRIDEiQ49A26.
TopDownProteomicsiQ49A26-2. [Q49A26-2]

PTM databases

iPTMnetiQ49A26.
PhosphoSitePlusiQ49A26.

Expressioni

Gene expression databases

BgeeiENSG00000140632.
ExpressionAtlasiQ49A26. baseline and differential.
GenevisibleiQ49A26. HS.

Organism-specific databases

HPAiCAB017022.
HPA048226.
HPA050136.

Interactioni

Subunit structurei

Interacts with MAPK14.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
C6orf165Q8IYR03EBI-2804292,EBI-749051
CCNCP248633EBI-2804292,EBI-395261
EIF1ADQ8N9N83EBI-2804292,EBI-750700
FHL2Q141923EBI-2804292,EBI-701903

GO - Molecular functioni

  • methylated histone binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124176. 75 interactors.
IntActiQ49A26. 37 interactors.
MINTiMINT-3063171.
STRINGi9606.ENSP00000322716.

Structurei

Secondary structure

1553
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni215 – 217Combined sources3
Helixi220 – 222Combined sources3
Beta strandi270 – 273Combined sources4
Helixi277 – 288Combined sources12
Beta strandi293 – 296Combined sources4
Helixi300 – 303Combined sources4
Helixi304 – 308Combined sources5
Helixi317 – 323Combined sources7
Beta strandi325 – 329Combined sources5
Helixi334 – 342Combined sources9
Helixi347 – 350Combined sources4
Beta strandi356 – 359Combined sources4
Helixi365 – 377Combined sources13
Beta strandi381 – 384Combined sources4
Beta strandi387 – 389Combined sources3
Helixi391 – 396Combined sources6
Beta strandi399 – 405Combined sources7
Helixi407 – 412Combined sources6
Helixi414 – 420Combined sources7
Beta strandi421 – 426Combined sources6
Helixi432 – 460Combined sources29
Helixi465 – 474Combined sources10
Helixi480 – 491Combined sources12
Beta strandi497 – 499Combined sources3
Helixi500 – 516Combined sources17
Helixi522 – 536Combined sources15
Helixi544 – 550Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2UYYX-ray2.50A/B/C/D261-553[»]
4GURX-ray2.51B152-268[»]
4GUSX-ray2.23B152-268[»]
4GUTX-ray2.00B152-268[»]
4GUUX-ray2.30B152-268[»]
4HSUX-ray1.99B152-268[»]
ProteinModelPortaliQ49A26.
SMRiQ49A26.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ49A26.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 66PWWPPROSITE-ProRule annotationAdd BLAST59

Domaini

The A.T hook DNA-binding domain is required for the interaction with MAPK14.
The PWWP domain probably mediates the binding to H3K36me3.

Sequence similaritiesi

Contains 1 A.T hook DNA-binding domain.Curated
Contains 1 PWWP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0409. Eukaryota.
KOG1904. Eukaryota.
COG2084. LUCA.
InParanoidiQ49A26.
OrthoDBiEOG091G0MNC.
PhylomeDBiQ49A26.
TreeFamiTF324195.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006115. 6PGDH_NADP-bd.
IPR016040. NAD(P)-bd_dom.
IPR029154. NADP-bd.
IPR000313. PWWP_dom.
[Graphical view]
PfamiPF14833. NAD_binding_11. 1 hit.
PF03446. NAD_binding_2. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q49A26-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVSLRLGD LVWGKLGRYP PWPGKIVNPP KDLKKPRGKK CFFVKFFGTE
60 70 80 90 100
DHAWIKVEQL KPYHAHKEEM IKINKGKRFQ QAVDAVEEFL RRAKGKDQTS
110 120 130 140 150
SHNSSDDKNR RNSSEERSRP NSGDEKRKLS LSEGKVKKNM GEGKKRVSSG
160 170 180 190 200
SSERGSKSPL KRAQEQSPRK RGRPPKDEKD LTIPESSTVK GMMAGPMAAF
210 220 230 240 250
KWQPTASEPV KDADPHFHHF LLSQTEKPAV CYQAITKKLK ICEEETGSTS
260 270 280 290 300
IQAADSTAVN GSITPTDKKI GFLGLGLMGS GIVSNLLKMG HTVTVWNRTA
310 320 330 340 350
EKCDLFIQEG ARLGRTPAEV VSTCDITFAC VSDPKAAKDL VLGPSGVLQG
360 370 380 390 400
IRPGKCYVDM STVDADTVTE LAQVIVSRGG RFLEAPVSGN QQLSNDGMLV
410 420 430 440 450
ILAAGDRGLY EDCSSCFQAM GKTSFFLGEV GNAAKMMLIV NMVQGSFMAT
460 470 480 490 500
IAEGLTLAHV TGQSQQTLLD ILNQGQLASI FLDQKCQNIL QGNFKPDFYL
510 520 530 540 550
KYIQKDLRLA IALGDAVNHP TPMAAAANEV YKRAKALDQS DNDMSAVYRA

YIH
Length:553
Mass (Da):60,556
Last modified:November 24, 2009 - v3
Checksum:i356598A73083203E
GO
Isoform 2 (identifier: Q49A26-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     228-244: Missing.

Note: No experimental confirmation available.
Show »
Length:536
Mass (Da):58,637
Checksum:i8A8C155A0BFA5CEB
GO
Isoform 5 (identifier: Q49A26-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     99-179: Missing.

Note: No experimental confirmation available.
Show »
Length:472
Mass (Da):51,508
Checksum:iA7B15666C92A58B3
GO
Isoform 3 (identifier: Q49A26-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     303-308: Missing.

Note: No experimental confirmation available.
Show »
Length:547
Mass (Da):59,837
Checksum:iD195E1EAEBB338C3
GO
Isoform 4 (identifier: Q49A26-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: Missing.

Note: No experimental confirmation available.
Show »
Length:484
Mass (Da):52,559
Checksum:iCDD318548A80FB06
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40K → E in AAH32855 (PubMed:15489334).Curated1
Sequence conflicti419A → T in AAH47223 (PubMed:15489334).Curated1
Sequence conflicti463Q → R in AAH47223 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_037403103N → D.Corresponds to variant rs34176249dbSNPEnsembl.1
Natural variantiVAR_037404459H → Q.6 PublicationsCorresponds to variant rs2085329dbSNPEnsembl.1
Natural variantiVAR_037405531Y → C.Corresponds to variant rs17703111dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0297061 – 69Missing in isoform 4. 1 PublicationAdd BLAST69
Alternative sequenceiVSP_03822299 – 179Missing in isoform 5. 1 PublicationAdd BLAST81
Alternative sequenceiVSP_029707228 – 244Missing in isoform 2. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_029708303 – 308Missing in isoform 3. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY352585 mRNA. Translation: AAQ57265.1.
AF244907 mRNA. Translation: AAQ14242.1.
AF326966 mRNA. Translation: AAK15524.1.
AK296842 mRNA. Translation: BAG59409.1.
AC020663 Genomic DNA. No translation available.
CH471112 Genomic DNA. Translation: EAW85252.1.
CH471112 Genomic DNA. Translation: EAW85257.1.
BC003693 mRNA. Translation: AAH03693.1.
BC032855 mRNA. Translation: AAH32855.1.
BC047223 mRNA. Translation: AAH47223.1.
BC064940 mRNA. Translation: AAH64940.1.
CCDSiCCDS10524.1. [Q49A26-1]
CCDS81945.1. [Q49A26-3]
RefSeqiNP_001295025.1. NM_001308096.1.
NP_115958.2. NM_032569.3.
XP_011521019.1. XM_011522717.1.
UniGeneiHs.387255.
Hs.731580.

Genome annotation databases

EnsembliENST00000321919; ENSP00000322716; ENSG00000140632.
ENST00000436648; ENSP00000390276; ENSG00000140632.
ENST00000591451; ENSP00000468328; ENSG00000140632.
GeneIDi84656.
KEGGihsa:84656.
UCSCiuc002cxx.5. human. [Q49A26-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY352585 mRNA. Translation: AAQ57265.1.
AF244907 mRNA. Translation: AAQ14242.1.
AF326966 mRNA. Translation: AAK15524.1.
AK296842 mRNA. Translation: BAG59409.1.
AC020663 Genomic DNA. No translation available.
CH471112 Genomic DNA. Translation: EAW85252.1.
CH471112 Genomic DNA. Translation: EAW85257.1.
BC003693 mRNA. Translation: AAH03693.1.
BC032855 mRNA. Translation: AAH32855.1.
BC047223 mRNA. Translation: AAH47223.1.
BC064940 mRNA. Translation: AAH64940.1.
CCDSiCCDS10524.1. [Q49A26-1]
CCDS81945.1. [Q49A26-3]
RefSeqiNP_001295025.1. NM_001308096.1.
NP_115958.2. NM_032569.3.
XP_011521019.1. XM_011522717.1.
UniGeneiHs.387255.
Hs.731580.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2UYYX-ray2.50A/B/C/D261-553[»]
4GURX-ray2.51B152-268[»]
4GUSX-ray2.23B152-268[»]
4GUTX-ray2.00B152-268[»]
4GUUX-ray2.30B152-268[»]
4HSUX-ray1.99B152-268[»]
ProteinModelPortaliQ49A26.
SMRiQ49A26.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124176. 75 interactors.
IntActiQ49A26. 37 interactors.
MINTiMINT-3063171.
STRINGi9606.ENSP00000322716.

PTM databases

iPTMnetiQ49A26.
PhosphoSitePlusiQ49A26.

Polymorphism and mutation databases

BioMutaiGLYR1.
DMDMi269849681.

Proteomic databases

EPDiQ49A26.
MaxQBiQ49A26.
PaxDbiQ49A26.
PeptideAtlasiQ49A26.
PRIDEiQ49A26.
TopDownProteomicsiQ49A26-2. [Q49A26-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000321919; ENSP00000322716; ENSG00000140632.
ENST00000436648; ENSP00000390276; ENSG00000140632.
ENST00000591451; ENSP00000468328; ENSG00000140632.
GeneIDi84656.
KEGGihsa:84656.
UCSCiuc002cxx.5. human. [Q49A26-1]

Organism-specific databases

CTDi84656.
GeneCardsiGLYR1.
HGNCiHGNC:24434. GLYR1.
HPAiCAB017022.
HPA048226.
HPA050136.
MIMi610660. gene.
neXtProtiNX_Q49A26.
PharmGKBiPA165450093.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0409. Eukaryota.
KOG1904. Eukaryota.
COG2084. LUCA.
InParanoidiQ49A26.
OrthoDBiEOG091G0MNC.
PhylomeDBiQ49A26.
TreeFamiTF324195.

Miscellaneous databases

ChiTaRSiGLYR1. human.
EvolutionaryTraceiQ49A26.
GenomeRNAii84656.
PROiQ49A26.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000140632.
ExpressionAtlasiQ49A26. baseline and differential.
GenevisibleiQ49A26. HS.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006115. 6PGDH_NADP-bd.
IPR016040. NAD(P)-bd_dom.
IPR029154. NADP-bd.
IPR000313. PWWP_dom.
[Graphical view]
PfamiPF14833. NAD_binding_11. 1 hit.
PF03446. NAD_binding_2. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLYR1_HUMAN
AccessioniPrimary (citable) accession number: Q49A26
Secondary accession number(s): B4DL47
, C9JJ40, C9JJ60, Q5U632, Q6P1Q2, Q6V3W7, Q9BTI1, Q9BXK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: November 24, 2009
Last modified: November 30, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The conserved NAD-binding sites and sequence similarity to plant dehydrogenases suggest that this protein may have oxidoreductase activity.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.