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Q49A26 (GLYR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative oxidoreductase GLYR1

EC=1.-.-.-
Alternative name(s):
3-hydroxyisobutyrate dehydrogenase-like protein
Cytokine-like nuclear factor N-PAC
Glyoxylate reductase 1 homolog
Nuclear protein NP60
Nuclear protein of 60 kDa
Gene names
Name:GLYR1
Synonyms:HIBDL, NP60
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May have oxidoreductase activity. Regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 and activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6. Recruited on chromatin, recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3). Ref.1 Ref.10

Subunit structure

Interacts with MAPK14. Ref.1

Subcellular location

Nucleus Ref.1.

Domain

The A.T hook DNA-binding domain is required for the interaction with MAPK14. Ref.1 Ref.10

The PWWP domain probably mediates the binding to H3K36me3. Ref.1 Ref.10

Miscellaneous

The conserved NAD-binding sites and sequence similarity to plant dehydrogenases suggest that this protein may have oxidoreductase activity.

Sequence similarities

Belongs to the 3-hydroxyisobutyrate dehydrogenase family. NP60 subfamily.

Contains 1 A.T hook DNA-binding domain.

Contains 1 PWWP domain.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q49A26-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q49A26-2)

The sequence of this isoform differs from the canonical sequence as follows:
     228-244: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q49A26-5)

The sequence of this isoform differs from the canonical sequence as follows:
     99-179: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q49A26-3)

The sequence of this isoform differs from the canonical sequence as follows:
     303-308: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q49A26-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 553553Putative oxidoreductase GLYR1
PRO_0000312121

Regions

Domain8 – 6659PWWP
DNA binding168 – 18013A.T hook
Nucleotide binding271 – 28515NAD By similarity

Sites

Binding site3621NAD By similarity
Binding site5051NAD By similarity

Amino acid modifications

Modified residue1301Phosphoserine Ref.11
Modified residue1671Phosphoserine Ref.8 Ref.13
Modified residue5401Phosphoserine Ref.11 Ref.13

Natural variations

Alternative sequence1 – 6969Missing in isoform 4.
VSP_029706
Alternative sequence99 – 17981Missing in isoform 5.
VSP_038222
Alternative sequence228 – 24417Missing in isoform 2.
VSP_029707
Alternative sequence303 – 3086Missing in isoform 3.
VSP_029708
Natural variant1031N → D.
Corresponds to variant rs34176249 [ dbSNP | Ensembl ].
VAR_037403
Natural variant4591H → Q. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.7
Corresponds to variant rs2085329 [ dbSNP | Ensembl ].
VAR_037404
Natural variant5311Y → C.
Corresponds to variant rs17703111 [ dbSNP | Ensembl ].
VAR_037405

Experimental info

Sequence conflict401K → E in AAH32855. Ref.7
Sequence conflict4191A → T in AAH47223. Ref.7
Sequence conflict4631Q → R in AAH47223. Ref.7

Secondary structure

.................................................... 553
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 24, 2009. Version 3.
Checksum: 356598A73083203E

FASTA55360,556
        10         20         30         40         50         60 
MAAVSLRLGD LVWGKLGRYP PWPGKIVNPP KDLKKPRGKK CFFVKFFGTE DHAWIKVEQL 

        70         80         90        100        110        120 
KPYHAHKEEM IKINKGKRFQ QAVDAVEEFL RRAKGKDQTS SHNSSDDKNR RNSSEERSRP 

       130        140        150        160        170        180 
NSGDEKRKLS LSEGKVKKNM GEGKKRVSSG SSERGSKSPL KRAQEQSPRK RGRPPKDEKD 

       190        200        210        220        230        240 
LTIPESSTVK GMMAGPMAAF KWQPTASEPV KDADPHFHHF LLSQTEKPAV CYQAITKKLK 

       250        260        270        280        290        300 
ICEEETGSTS IQAADSTAVN GSITPTDKKI GFLGLGLMGS GIVSNLLKMG HTVTVWNRTA 

       310        320        330        340        350        360 
EKCDLFIQEG ARLGRTPAEV VSTCDITFAC VSDPKAAKDL VLGPSGVLQG IRPGKCYVDM 

       370        380        390        400        410        420 
STVDADTVTE LAQVIVSRGG RFLEAPVSGN QQLSNDGMLV ILAAGDRGLY EDCSSCFQAM 

       430        440        450        460        470        480 
GKTSFFLGEV GNAAKMMLIV NMVQGSFMAT IAEGLTLAHV TGQSQQTLLD ILNQGQLASI 

       490        500        510        520        530        540 
FLDQKCQNIL QGNFKPDFYL KYIQKDLRLA IALGDAVNHP TPMAAAANEV YKRAKALDQS 

       550 
DNDMSAVYRA YIH 

« Hide

Isoform 2 [UniParc].

Checksum: 8A8C155A0BFA5CEB
Show »

FASTA53658,637
Isoform 5 [UniParc].

Checksum: A7B15666C92A58B3
Show »

FASTA47251,508
Isoform 3 [UniParc].

Checksum: D195E1EAEBB338C3
Show »

FASTA54759,837
Isoform 4 [UniParc].

Checksum: CDD318548A80FB06
Show »

FASTA48452,559

References

« Hide 'large scale' references
[1]"Nuclear protein NP60 regulates p38 MAPK activity."
Fu J., Yang Z., Wei J., Han J., Gu J.
J. Cell Sci. 119:115-123(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH MAPK14, VARIANT GLN-459.
[2]"Molecular characterization of a novel human PWWP domain containing protein with homology to 3-hydroxyisobutyrate dehydrogenase."
Watari Y., Tsujino T., Nonaka H., Shirai Y., Saito N., Yokoyama M.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT GLN-459.
[3]"A novel cytokine-like nuclear factor, N-PAC."
New L., Han J.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), VARIANT GLN-459.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5), VARIANT GLN-459.
Tissue: Tongue.
[5]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLN-459.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 14-553 (ISOFORM 2), VARIANT GLN-459.
Tissue: Brain, Lymph, Placenta and Testis.
[8]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers."
Vermeulen M., Eberl H.C., Matarese F., Marks H., Denissov S., Butter F., Lee K.K., Olsen J.V., Hyman A.A., Stunnenberg H.G., Mann M.
Cell 142:967-980(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN PWWP, FUNCTION.
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130 AND SER-540, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167 AND SER-540, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"The structure of the cytokine-like nuclear factor N-PAC."
Structural genomics consortium (SGC)
Submitted (FEB-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 261-553 IN COMPLEX WITH NAD ANALOG.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY352585 mRNA. Translation: AAQ57265.1.
AF244907 mRNA. Translation: AAQ14242.1.
AF326966 mRNA. Translation: AAK15524.1.
AK296842 mRNA. Translation: BAG59409.1.
AC020663 Genomic DNA. No translation available.
CH471112 Genomic DNA. Translation: EAW85252.1.
CH471112 Genomic DNA. Translation: EAW85257.1.
BC003693 mRNA. Translation: AAH03693.1.
BC032855 mRNA. Translation: AAH32855.1.
BC047223 mRNA. Translation: AAH47223.1.
BC064940 mRNA. Translation: AAH64940.1.
RefSeqNP_115958.2. NM_032569.3.
XP_005255697.2. XM_005255640.2.
UniGeneHs.387255.
Hs.731580.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2UYYX-ray2.50A/B/C/D261-553[»]
4GURX-ray2.51B152-268[»]
4GUSX-ray2.23B152-268[»]
4GUTX-ray2.00B152-268[»]
4GUUX-ray2.30B152-268[»]
4HSUX-ray1.99B152-268[»]
ProteinModelPortalQ49A26.
SMRQ49A26. Positions 7-87, 262-553.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124176. 9 interactions.
IntActQ49A26. 7 interactions.
MINTMINT-3063171.

PTM databases

PhosphoSiteQ49A26.

Polymorphism databases

DMDM269849681.

Proteomic databases

PaxDbQ49A26.
PRIDEQ49A26.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000321919; ENSP00000322716; ENSG00000140632. [Q49A26-1]
ENST00000381983; ENSP00000371413; ENSG00000140632. [Q49A26-2]
ENST00000436648; ENSP00000390276; ENSG00000140632. [Q49A26-5]
ENST00000591451; ENSP00000468328; ENSG00000140632. [Q49A26-3]
GeneID84656.
KEGGhsa:84656.
UCSCuc002cxx.4. human. [Q49A26-1]
uc002cxz.1. human. [Q49A26-2]
uc002cya.2. human. [Q49A26-3]
uc010uxv.1. human. [Q49A26-5]

Organism-specific databases

CTD84656.
GeneCardsGC16M004855.
HGNCHGNC:24434. GLYR1.
HPACAB017022.
HPA048226.
HPA050136.
MIM610660. gene.
neXtProtNX_Q49A26.
PharmGKBPA165450093.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2084.
InParanoidQ49A26.
OMATVAGFKW.
OrthoDBEOG7992RZ.
PhylomeDBQ49A26.
TreeFamTF324195.

Gene expression databases

ArrayExpressQ49A26.
BgeeQ49A26.
GenevestigatorQ49A26.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR006115. 6PGDH_NADP-bd.
IPR017956. AT_hook_DNA-bd_motif.
IPR013328. DH_multihelical.
IPR015815. HIBADH-type.
IPR016040. NAD(P)-bd_dom.
IPR000313. PWWP_dom.
[Graphical view]
PANTHERPTHR22981. PTHR22981. 1 hit.
PfamPF03446. NAD_binding_2. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
SMARTSM00384. AT_hook. 1 hit.
[Graphical view]
SUPFAMSSF48179. SSF48179. 1 hit.
PROSITEPS50812. PWWP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGLYR1. human.
EvolutionaryTraceQ49A26.
GenomeRNAi84656.
NextBio74618.
PROQ49A26.
SOURCESearch...

Entry information

Entry nameGLYR1_HUMAN
AccessionPrimary (citable) accession number: Q49A26
Secondary accession number(s): B4DL47 expand/collapse secondary AC list , C9JJ40, C9JJ60, Q5U632, Q6P1Q2, Q6V3W7, Q9BTI1, Q9BXK2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: November 24, 2009
Last modified: April 16, 2014
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM