ID   SYMM_MOUSE              Reviewed;         586 AA.
AC   Q499X9; Q3T9W7; Q8BWR9; Q8BXY1;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2006, sequence version 2.
DT   16-JUN-2009, entry version 40.
DE   RecName: Full=Methionyl-tRNA synthetase, mitochondrial;
DE            EC=6.1.1.10;
DE   AltName: Full=Mitochondrial methionine--tRNA ligase;
DE            Short=MtMetRS;
DE   AltName: Full=Methionine--tRNA ligase 2;
DE   Flags: Precursor;
GN   Name=Mars2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J, and NOD; TISSUE=Cerebellum, Liver, and Spleen;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N-3; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- CATALYTIC ACTIVITY: ATP + L-methionine + tRNA(Met) = AMP +
CC       diphosphate + L-methionyl-tRNA(Met).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix (By similarity).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; AK042888; BAC31393.1; ALT_INIT; mRNA.
DR   EMBL; AK050196; BAC34119.1; -; mRNA.
DR   EMBL; AK172240; BAE42903.1; -; mRNA.
DR   EMBL; BC099669; AAH99669.1; -; mRNA.
DR   IPI; IPI00226952; -.
DR   RefSeq; NP_780648.1; -.
DR   UniGene; Mm.19223; -.
DR   Ensembl; ENSMUSG00000046994; Mus musculus.
DR   GeneID; 212679; -.
DR   KEGG; mmu:212679; -.
DR   NMPDR; fig|10090.3.peg.356; -.
DR   MGI; MGI:2444136; Mars2.
DR   HOGENOM; Q499X9; -.
DR   HOVERGEN; Q499X9; -.
DR   OMA; Q499X9; KMSKYAD.
DR   BRENDA; 6.1.1.10; 244.
DR   NextBio; 373664; -.
DR   ArrayExpress; Q499X9; -.
DR   Bgee; Q499X9; -.
DR   CleanEx; MM_MARS2; -.
DR   GermOnline; ENSMUSG00000046994; Mus musculus.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:EC.
DR   GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro.
DR   InterPro; IPR015413; aa-tRNA-synt_I.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002304; Met-tRNA-synth_Ia.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR014758; tRNA-synt_met_N.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR01041; TRNASYNTHMET.
DR   TIGRFAMs; TIGR00398; metG; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; FALSE_NEG.
PE   2: Evidence at transcript level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Mitochondrion;
KW   Nucleotide-binding; Protein biosynthesis; Transit peptide.
FT   TRANSIT       1     46       Mitochondrion (Potential).
FT   CHAIN        47    586       Methionyl-tRNA synthetase, mitochondrial.
FT                                /FTId=PRO_0000045494.
FT   MOTIF        45     55       "HIGH" region.
FT   MOTIF       340    344       "KMSKS" region.
FT   BINDING     343    343       ATP (By similarity).
FT   CONFLICT    306    306       L -> F (in Ref. 2; AAH99669).
SQ   SEQUENCE   586 AA;  65805 MW;  639695ECEE842EFB CRC64;
     MLRQCARWVL TRTRFGRGCR RYGSCSPSAS GDAGEARAYF TTPIFYVNAA PHIGHLYSAL
     LADALCRHRR LRVPGSASTR FSTGTDEHGL KIQQAAATAG LAPIELCDRV SAQFLQLFRE
     ADISSTDFIR TTEARHRVAV QHFWGVLEAR GLLYKGIYEG WYCASDECFL PEAKVTRQVG
     PSGDPCPVSL ESGHPVSWTK EENYIFKLSQ FREPLQRWLG NNPQAITPEP FHQAVLQWLE
     EELPDLSVSR RSSHLHWGIP VPGDDSQTIY VWLDALVNYL TVVGYPDADF KSWWPATSHI
     IGKDILKFHA IYWPALLLGA GLRPPHRIYV HSHWTVSGQK MSKSLGNVVD PRTCLDRYTV
     DGFRYFLLRQ GVPNWDCDYY DEKVVKLLDS ELADALGGLL NRCTAYRINP SGTYPSFCAA
     CFPSEPGLTG PSVRVQAEDY ALVTAVATLP KLVAGYYNDF QIYKALEAVS SCVRQTNGFV
     QRHAPWKLNW ESPEDAPWLG TVLHVALECL RVFGTLLQPV TPNLADKLLS RLGVSTTERG
     LGELYFLPRF YGHPCPFEGR KLGPDTGLLF PRLDQSRTRL VKAHRT
//