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Protein

Gamma-interferon-inducible lysosomal thiol reductase

Gene

Ifi30

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation (By similarity).By similarity

Miscellaneous

Both precursor form and mature form have thiol reductase activity.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processImmunity

Enzyme and pathway databases

ReactomeiR-RNO-2132295 MHC class II antigen presentation

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-interferon-inducible lysosomal thiol reductase (EC:1.8.-.-By similarity)
Alternative name(s):
Interferon gamma inducible protein 30
Gene namesi
Name:Ifi30
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi1310758 Ifi30

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000040622725 – 54Removed in mature formBy similarityAdd BLAST30
ChainiPRO_000040622855 – 230Gamma-interferon-inducible lysosomal thiol reductaseAdd BLAST176
PropeptideiPRO_0000406229231 – 248Removed in mature formBy similarityAdd BLAST18

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi69 ↔ 72Redox-activeBy similarity
Glycosylationi92N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi105N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated. Sugar chains contain mannose-6-phosphate (By similarity).By similarity
Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ499T2
PRIDEiQ499T2

PTM databases

PhosphoSitePlusiQ499T2

Expressioni

Gene expression databases

BgeeiENSRNOG00000019387
GenevisibleiQ499T2 RN

Interactioni

Subunit structurei

Dimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026225

Structurei

3D structure databases

SMRiQ499T2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GILT family.Curated

Keywords - Domaini

Redox-active center, Signal

Phylogenomic databases

eggNOGiKOG3160 Eukaryota
ENOG4111IDT LUCA
GeneTreeiENSGT00390000010450
HOGENOMiHOG000238423
HOVERGENiHBG005837
InParanoidiQ499T2
KOiK08059
OMAiVSGRWEF
OrthoDBiEOG091G0IMC
PhylomeDBiQ499T2
TreeFamiTF315141

Family and domain databases

InterProiView protein in InterPro
IPR004911 Interferon-induced_GILT
PANTHERiPTHR13234 PTHR13234, 1 hit
PfamiView protein in Pfam
PF03227 GILT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q499T2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCSPLVPFL SLLLLLFLPE VPRAATASLP QGSSEGAATC KAHDLCLFGP
60 70 80 90 100
RRLLSAPPVN VSLYYESLCG ACRYFLVRNL FPTWLMVMEI MNITLVPYGN
110 120 130 140 150
AQERNVSGTW EFTCQHGELE CKLNKVEACL LDKLEKEAAF LTIVCMEEME
160 170 180 190 200
DMEKKLGPCL QLYVPEVSPE SIMECATGKR GTELMHENAQ LTDALQPPHE
210 220 230 240
YVPWVLVNEK PLTDPSQLLS SVCELYQGTE KPDICSSMAD APREVCYK
Length:248
Mass (Da):27,644
Last modified:September 13, 2005 - v1
Checksum:i12A82B9AF6E2E3B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474031 Genomic DNA Translation: EDL90735.1
BC099774 mRNA Translation: AAH99774.1
BC088256 mRNA Translation: AAH88256.1
RefSeqiNP_001025197.1, NM_001030026.1
UniGeneiRn.3370

Genome annotation databases

EnsembliENSRNOT00000026225; ENSRNOP00000026225; ENSRNOG00000019387
GeneIDi290644
KEGGirno:290644

Similar proteinsi

Entry informationi

Entry nameiGILT_RAT
AccessioniPrimary (citable) accession number: Q499T2
Secondary accession number(s): Q5I0J7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: September 13, 2005
Last modified: May 23, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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