Gamma-interferon-inducible lysosomal thiol reductase precursor

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Q499T2 (GILT_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 43. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Gamma-interferon-inducible lysosomal thiol reductase
EC=1.8.-.-

Alternative name(s):
Interferon gamma inducible protein 30

Gene names

Name:

Ifi30

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation By similarity.
Subunit structure	Dimer; disulfide-linked By similarity.
Subcellular location	Secreted By similarity. Lysosome By similarity.
Post-translational modification	N-glycosylated. Sugar chains contain mannose-6-phosphate By similarity. Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases By similarity.
Miscellaneous	Both precursor form and mature form have thiol reductase activity By similarity.
Sequence similarities	Belongs to the GILT family.

Ontologies

Keywords
Biological process	Immunity
Cellular component	Lysosome Secreted
Domain	Redox-active center Signal
Molecular function	Oxidoreductase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class I Inferred from sequence or structural similarity. Source: UniProtKB antigen processing and presentation of exogenous peptide antigen via MHC class II Inferred from electronic annotation. Source: Compara negative regulation of fibroblast proliferation Inferred from electronic annotation. Source: Compara protein stabilization Inferred from electronic annotation. Source: Compara
Cellular_component	cell junction Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	oxidoreductase activity, acting on a sulfur group of donors Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

Potential

Propeptide

25 – 54

Removed in mature form By similarity

PRO_0000406227

Chain

55 – 230

176

Gamma-interferon-inducible lysosomal thiol reductase

PRO_0000406228

Propeptide

231 – 248

Removed in mature form By similarity

PRO_0000406229

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

105

N-linked (GlcNAc...) Potential

Disulfide bond

69 ↔ 72

Redox-active By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q499T2 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 12A82B9AF6E2E3B4
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FASTA

248

27,644

        10         20         30         40         50         60 
MSCSPLVPFL SLLLLLFLPE VPRAATASLP QGSSEGAATC KAHDLCLFGP RRLLSAPPVN 

        70         80         90        100        110        120 
VSLYYESLCG ACRYFLVRNL FPTWLMVMEI MNITLVPYGN AQERNVSGTW EFTCQHGELE 

       130        140        150        160        170        180 
CKLNKVEACL LDKLEKEAAF LTIVCMEEME DMEKKLGPCL QLYVPEVSPE SIMECATGKR 

       190        200        210        220        230        240 
GTELMHENAQ LTDALQPPHE YVPWVLVNEK PLTDPSQLLS SVCELYQGTE KPDICSSMAD 


APREVCYK

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References

[1]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH474031 Genomic DNA. Translation: EDL90735.1. BC099774 mRNA. Translation: AAH99774.1. BC088256 mRNA. Translation: AAH88256.1.
IPI	IPI00196145.
RefSeq	NP_001025197.1. NM_001030026.1.
UniGene	Rn.3370.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000026225.
PTM databases
PhosphoSite	Q499T2.
Proteomic databases
PaxDb	Q499T2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026225; ENSRNOP00000026225; ENSRNOG00000019387.
GeneID	290644.
KEGG	rno:290644.
Organism-specific databases
CTD	10437.
RGD	1310758. Ifi30.
Phylogenomic databases
eggNOG	NOG315223.
GeneTree	ENSGT00390000010450.
HOGENOM	HOG000238423.
HOVERGEN	HBG005837.
InParanoid	Q5I0J7.
KO	K08059.
OMA	VNVSLYY.
OrthoDB	EOG4HHP3H.
Gene expression databases
Genevestigator	Q499T2.
Family and domain databases
InterPro	IPR004911. Interferon-induced_GILT. [Graphical view]
PANTHER	PTHR13234. PTHR13234. 1 hit.
Pfam	PF03227. GILT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	631392.

Entry information

Entry name

GILT_RAT

Accession

Primary (citable) accession number: Q499T2
Secondary accession number(s): Q5I0J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 5, 2011
Last sequence update:	September 13, 2005
Last modified:	April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents