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Q499S5

- MMP11_RAT

UniProt

Q499S5 - MMP11_RAT

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Protein

Stromelysin-3

Gene

Mmp11

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

May play an important role in the progression of epithelial malignancies.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+Note: Binds 1 Ca(2+) ion per subunit.
  • Zn2+Note: Binds 2 Zn(2+) ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi84 – 841Zinc 2; in inhibited formBy similarity
Metal bindingi168 – 1681Zinc 1By similarity
Metal bindingi170 – 1701Zinc 1By similarity
Metal bindingi175 – 1751CalciumBy similarity
Metal bindingi176 – 1761Calcium; via carbonyl oxygenBy similarity
Metal bindingi178 – 1781Calcium; via carbonyl oxygenBy similarity
Metal bindingi180 – 1801Calcium; via carbonyl oxygenBy similarity
Metal bindingi183 – 1831Zinc 1By similarity
Metal bindingi196 – 1961Zinc 1By similarity
Metal bindingi218 – 2181Zinc 2; catalyticBy similarity
Active sitei219 – 2191PROSITE-ProRule annotation
Metal bindingi222 – 2221Zinc 2; catalyticBy similarity
Metal bindingi228 – 2281Zinc 2; catalyticBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. metalloendopeptidase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. basement membrane organization Source: Ensembl
  2. collagen catabolic process Source: UniProtKB-KW
  3. collagen fibril organization Source: Ensembl
  4. multicellular organismal development Source: InterPro
  5. negative regulation of fat cell differentiation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Collagen degradation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198590. Activation of Matrix Metalloproteinases.
REACT_199177. Collagen degradation.

Protein family/group databases

MEROPSiM10.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Stromelysin-3 (EC:3.4.24.-)
Short name:
SL-3
Short name:
ST3
Alternative name(s):
Matrix metalloproteinase-11
Short name:
MMP-11
Gene namesi
Name:Mmp11
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 20

Organism-specific databases

RGDi3099. Mmp11.

Subcellular locationi

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence AnalysisAdd
BLAST
Propeptidei36 – 10166Activation peptideBy similarityPRO_0000042649Add
BLAST
Chaini102 – 491390Stromelysin-3PRO_0000042650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi297 ↔ 483By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Zymogen

Proteomic databases

PaxDbiQ499S5.
PRIDEiQ499S5.

PTM databases

PhosphoSiteiQ499S5.

Expressioni

Tissue specificityi

Highly expressed in ovary and uterus.1 Publication

Inductioni

In skin fibroblasts of superficial dermis upon skin lesion with highest level between days 5-10.1 Publication

Gene expression databases

GenevestigatoriQ499S5.

Interactioni

Protein-protein interaction databases

BioGridi247514. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ499S5.
SMRiQ499S5. Positions 102-264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati298 – 34245Hemopexin 1Add
BLAST
Repeati343 – 38543Hemopexin 2Add
BLAST
Repeati387 – 43549Hemopexin 3Add
BLAST
Repeati436 – 48348Hemopexin 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi82 – 898Cysteine switchBy similarity

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG236137.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiQ499S5.
KOiK07993.
OMAiVDTNEIA.
OrthoDBiEOG7XPZ57.
PhylomeDBiQ499S5.

Family and domain databases

Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR028705. Stromelysin-3.
[Graphical view]
PANTHERiPTHR10201:SF20. PTHR10201:SF20. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF50923. SSF50923. 1 hit.
PROSITEiPS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q499S5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARAACLLRA ISRALLLPLP LLLLLLLLLP PQLMARARPP ENHRHRPVKR
60 70 80 90 100
VPQLLPAALP NSLPSVPASH WVPGPASSSR PLRCGVPDPP DVLNARNRQK
110 120 130 140 150
RFVLSGGRWE KTDLTYRILR FPWQLVREQV RQTVAEALRV WSEVTPLTFT
160 170 180 190 200
EVHEGRADIM IDFTRYWHGD NLPFDGPGGI LAHAFFPKTH REGDVHFDYD
210 220 230 240 250
ETWTIGDKGT DLLQVAAHEF GHVLGLQHTT AAKALMSPFY TFRYPLSLSP
260 270 280 290 300
DDRRGIQHLY GRPQLTPTSP TPTLSSQAGT DTNEIALQEP EVPPEVCETS
310 320 330 340 350
FDAVSTIRGE LFFFKAGFVW RLRSGQLQPG YPALASRHWQ GLPSPVDAAF
360 370 380 390 400
EDAQGQIWFF QGAQYWVYDG EKPVLGPAPL SKLGLQGSPV HAALVWGPEK
410 420 430 440 450
NKIYFFRGGD YWRFHPRTQR VDNPVPRRTT DWRGVPSEID AAFQDAEGYA
460 470 480 490
YFLRGHLYWK FDPVKVKVLE SFPRPIGPDF FDCAEPANTF R
Length:491
Mass (Da):55,511
Last modified:September 13, 2005 - v1
Checksum:iB0C5B191A623FD41
GO

Sequence cautioni

The sequence AAC53061.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH99781.2 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46034 mRNA. Translation: AAC53061.1. Different initiation.
BC099781 mRNA. Translation: AAH99781.2. Different initiation.
RefSeqiNP_037112.2. NM_012980.2.
XP_008772050.1. XM_008773828.1.
UniGeneiRn.11123.

Genome annotation databases

EnsembliENSRNOT00000031400; ENSRNOP00000035334; ENSRNOG00000028344.
GeneIDi103694874.
25481.
KEGGirno:25481.
UCSCiRGD:3099. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46034 mRNA. Translation: AAC53061.1 . Different initiation.
BC099781 mRNA. Translation: AAH99781.2 . Different initiation.
RefSeqi NP_037112.2. NM_012980.2.
XP_008772050.1. XM_008773828.1.
UniGenei Rn.11123.

3D structure databases

ProteinModelPortali Q499S5.
SMRi Q499S5. Positions 102-264.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247514. 2 interactions.

Protein family/group databases

MEROPSi M10.007.

PTM databases

PhosphoSitei Q499S5.

Proteomic databases

PaxDbi Q499S5.
PRIDEi Q499S5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000031400 ; ENSRNOP00000035334 ; ENSRNOG00000028344 .
GeneIDi 103694874.
25481.
KEGGi rno:25481.
UCSCi RGD:3099. rat.

Organism-specific databases

CTDi 4320.
RGDi 3099. Mmp11.

Phylogenomic databases

eggNOGi NOG236137.
GeneTreei ENSGT00760000118870.
HOGENOMi HOG000217927.
HOVERGENi HBG052484.
InParanoidi Q499S5.
KOi K07993.
OMAi VDTNEIA.
OrthoDBi EOG7XPZ57.
PhylomeDBi Q499S5.

Enzyme and pathway databases

Reactomei REACT_198590. Activation of Matrix Metalloproteinases.
REACT_199177. Collagen degradation.

Miscellaneous databases

NextBioi 606819.
PROi Q499S5.

Gene expression databases

Genevestigatori Q499S5.

Family and domain databases

Gene3Di 2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProi IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR028705. Stromelysin-3.
[Graphical view ]
PANTHERi PTHR10201:SF20. PTHR10201:SF20. 1 hit.
Pfami PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
[Graphical view ]
PIRSFi PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSi PR00138. MATRIXIN.
SMARTi SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF50923. SSF50923. 1 hit.
PROSITEi PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rat stromelysin 3: cDNA cloning from healing skin wound, activation by furin and expression in rat tissues."
    Okada A., Saez S., Misumi Y., Basset P.
    Gene 185:187-193(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION, PROTEOLYTIC PROCESSING.
    Strain: Wistar.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiMMP11_RAT
AccessioniPrimary (citable) accession number: Q499S5
Secondary accession number(s): P97568
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: September 13, 2005
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3