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Protein

UBX domain-containing protein 1

Gene

Ubxn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Ubiquitin-binding protein that interacts with the BRCA1-BARD1 heterodimer, and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1, leads to inhibit the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
UBX domain-containing protein 1
Alternative name(s):
SAPK substrate protein 1
Gene namesi
Name:Ubxn1
Synonyms:Saks1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1309471. Ubxn1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: Ensembl
  • dendrite Source: RGD
  • neuronal cell body Source: RGD
  • nucleus Source: RGD
  • proteasome complex Source: Ensembl
  • VCP-NPL4-UFD1 AAA ATPase complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 297296UBX domain-containing protein 1PRO_0000248998Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei199 – 1991PhosphoserineBy similarity
Modified residuei200 – 2001Phosphoserine; by MAPK12By similarity
Modified residuei207 – 2071PhosphothreonineBy similarity
Modified residuei229 – 2291PhosphothreonineBy similarity
Modified residuei270 – 2701PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ499N6.
PRIDEiQ499N6.

PTM databases

iPTMnetiQ499N6.
PhosphoSiteiQ499N6.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019666.
GenevisibleiQ499N6. RN.

Interactioni

Subunit structurei

Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B. Interacts with HOMER2 (By similarity). Interacts directly with VCP. Interacts with BRCA1 and BARD1; interaction takes place when BRCA1 is not autoubiquitinated bur is strongly enhanced in the presence of autoubiquitinated BRCA1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026651.

Structurei

3D structure databases

ProteinModelPortaliQ499N6.
SMRiQ499N6. Positions 2-57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 4241UBAPROSITE-ProRule annotationAdd
BLAST
Domaini209 – 29183UBXPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 297255Interaction with BRCA1By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili86 – 17287Sequence analysisAdd
BLAST

Domaini

The UBA domain specifically recognizes and binds 'Lys-6'-linked polyubiquitin chains.By similarity

Sequence similaritiesi

Contains 1 UBA domain.PROSITE-ProRule annotation
Contains 1 UBX domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2689. Eukaryota.
ENOG4111HH6. LUCA.
GeneTreeiENSGT00530000063500.
HOGENOMiHOG000188321.
HOVERGENiHBG082310.
InParanoidiQ499N6.
OMAiHTEVEYH.
OrthoDBiEOG091G0N23.
PhylomeDBiQ499N6.
TreeFamiTF313944.

Family and domain databases

InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q499N6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELTALESL IEMGFPRGRA EKALALTGNQ GIEAAMDWLM EHEDDPDVDE
60 70 80 90 100
PLETPLSHIL GREPTPSEQV GPEGSGSAAG ESKPVLTEEE RQEQTKRMLE
110 120 130 140 150
LVAQKQRERE EREEREALER EKQRRRQGQE LSAARQKLQE DEIRRAAEER
160 170 180 190 200
RREKAEELAA RQRVREKIER DKAERAQKYG GTVGSRSSPP ATDPGPVPSS
210 220 230 240 250
PRQEPPTKRE YDQCRIQVRL PDGTSLTQTF RAREQLAAVR LYVELHRGEE
260 270 280 290
PGQDQDPVQL LSGFPRRAFS EADMERPLQE LGLVPSAVLI VAKKCPS
Length:297
Mass (Da):33,582
Last modified:September 5, 2006 - v2
Checksum:iABF2A4F385293DCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03002578 Genomic DNA. No translation available.
UniGeneiRn.198539.

Genome annotation databases

EnsembliENSRNOT00000026651; ENSRNOP00000026651; ENSRNOG00000019666.
UCSCiRGD:1309471. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03002578 Genomic DNA. No translation available.
UniGeneiRn.198539.

3D structure databases

ProteinModelPortaliQ499N6.
SMRiQ499N6. Positions 2-57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026651.

PTM databases

iPTMnetiQ499N6.
PhosphoSiteiQ499N6.

Proteomic databases

PaxDbiQ499N6.
PRIDEiQ499N6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026651; ENSRNOP00000026651; ENSRNOG00000019666.
UCSCiRGD:1309471. rat.

Organism-specific databases

RGDi1309471. Ubxn1.

Phylogenomic databases

eggNOGiKOG2689. Eukaryota.
ENOG4111HH6. LUCA.
GeneTreeiENSGT00530000063500.
HOGENOMiHOG000188321.
HOVERGENiHBG082310.
InParanoidiQ499N6.
OMAiHTEVEYH.
OrthoDBiEOG091G0N23.
PhylomeDBiQ499N6.
TreeFamiTF313944.

Miscellaneous databases

PROiQ499N6.

Gene expression databases

BgeeiENSRNOG00000019666.
GenevisibleiQ499N6. RN.

Family and domain databases

InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBXN1_RAT
AccessioniPrimary (citable) accession number: Q499N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: September 7, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.